FlyBase Allele Report: Zzzz\CGG[100.5'UTR.UAS.GFP]

General Information

Symbol

Zzzz\CGG^{100.5'UTR.UAS.GFP}

Species

a. artificial

Name

FlyBase ID

FBal0286134

Feature type

allele

Associated gene

Zzzz\CGG

Associated Insertion(s)

Carried in Construct

P{UAS-CGG100-5'UTR-GFP}

Transgenic product class

trinucleotide_repeat_expansion

(Todd et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

trinucleotide_repeat_expansion

(Todd et al., 2013)

Mutagen

in vitro construct

(Todd et al., 2013)

Progenitor genotype

Carried in construct

P{UAS-CGG100-5'UTR-GFP}

Cytology

Description

UASt regulatory sequences drive expression of 100 CGG repeats (derived from the 5'UTR of Hsap\FMR1) followed by the Avic\GFP coding region in the GGC glycine-encoding (+1) frame.

(Todd et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Zzzz\CGG

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of fragile X-associated tremor/ataxia syndrome

CEA

(Oh et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of fragile X-associated tremor/ataxia syndrome

is exacerbated by Prosβ6^1.B.UAS

(Oh et al., 2015)

is exacerbated by Prosβ2^1.UAS

(Oh et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal, with Scer\GAL4^Act5C.PU

(Todd et al., 2013)

visible, with Scer\GAL4^GMR.PF

(Todd et al., 2013)

visible | adult stage, with Scer\GAL4^GMR.long

(Oh et al., 2015)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.long

(Oh et al., 2015)

eye, with Scer\GAL4^GMR.PF

(Todd et al., 2013)

ommatidium, with Scer\GAL4^GMR.long

(Oh et al., 2015)

Detailed Description

Statement

Reference

Expression of Zzzz\CGG^{100.5'UTR.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.long leads to moderate rough eye phenotype and formation of inclusions in the ommatidia in adult flies maintained at 28[o]C.

(Oh et al., 2015)

Flies expressing Zzzz\CGG^{100.5'UTR.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PF have a moderate rough eye phenotype.

(Todd et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.long, Zzzz\CGG^{100.5'UTR.UAS.GFP} has visible | adult stage phenotype, enhanceable by Prosβ6^1.B.UAS, Scer\GAL4^GMR.long

(Oh et al., 2015)

Scer\GAL4^GMR.long, Zzzz\CGG^{100.5'UTR.UAS.GFP} has visible | adult stage phenotype, enhanceable by Prosβ2^1.UAS, Scer\GAL4^GMR.long

(Oh et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.long, Zzzz\CGG^{100.5'UTR.UAS.GFP} has visible | adult stage phenotype, suppressible by Hsc70-4^UAS.cEa, Scer\GAL4^GMR.long

(Oh et al., 2015)

Enhancer of

Statement

Reference

Zzzz\CGG^{100.5'UTR.UAS.GFP}, Scer\GAL4^GMR.long is an enhancer of visible | adult stage phenotype of Prosβ2^1.UAS, Scer\GAL4^GMR.long

(Oh et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.long, Zzzz\CGG^{100.5'UTR.UAS.GFP} has eye phenotype, enhanceable by Prosβ6^1.B.UAS, Scer\GAL4^GMR.long

(Oh et al., 2015)

Scer\GAL4^GMR.long, Zzzz\CGG^{100.5'UTR.UAS.GFP} has eye phenotype, enhanceable by Prosβ2^1.UAS, Scer\GAL4^GMR.long

(Oh et al., 2015)

Scer\GAL4^GMR.long, Zzzz\CGG^{100.5'UTR.UAS.GFP} has ommatidium phenotype, enhanceable by Prosβ2^1.UAS, Scer\GAL4^GMR.long

(Oh et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.long, Zzzz\CGG^{100.5'UTR.UAS.GFP} has eye phenotype, suppressible by Hsc70-4^UAS.cEa, Scer\GAL4^GMR.long

(Oh et al., 2015)

Scer\GAL4^GMR.long, Zzzz\CGG^{100.5'UTR.UAS.GFP} has ommatidium phenotype, suppressible by Hsc70-4^UAS.cEa, Scer\GAL4^GMR.long

(Oh et al., 2015)

Enhancer of

Statement

Reference

Zzzz\CGG^{100.5'UTR.UAS.GFP}, Scer\GAL4^GMR.long is an enhancer of eye phenotype of Prosβ2^1.UAS, Scer\GAL4^GMR.long

(Oh et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The eye degeneration phenotype as well as formation of inclusions in the ommatidia observed in adult flies expressing Zzzz\CGG^{100.5'UTR.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.long (and maintained at 28[o]C) is significantly enhanced by co-expression of Prosβ2^1.Scer\UAS (which on its own also induces rough eye phenotype) and suppressed by co-expression of Hsc70-4^Scer\UAS.cEa, the eye degeneration is most severe when two copies of the Prosβ2^1.Scer\UAS transgene are used. The Zzzz\CGG^{100.5'UTR.Scer\UAS.T:Avic\GFP}-induced rough eye phenotype can also be enhanced by co-expression of Prosβ6^1.B.Scer\UAS.

(Oh et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Zzzz\CGG^{100.5'UTR.Scer\UAS.T:Avic\GFP}

Zzzz\CGG^{100.5'UTR.UAS.GFP}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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