FB2024_03 , released June 25, 2024
Allele: Dmel\pkAct5C.pk.EGFP
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General Information
Symbol
Dmel\pkAct5C.pk.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0287885
Feature type
allele
Associated gene
Dmel\pk
Associated Insertion(s)
Carried in Construct
P{Act5C(-FRT)pk.pk.EGFP}
Key Links
Transgenic product class
wild_type
(Strutt et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Strutt et al., 2013)
Mutagen
FLPase
(Strutt et al., 2013)
Progenitor genotype
pkAct5C.pk.FRT.EGFP
(Strutt et al., 2013)
Carried in construct
P{Act5C(-FRT)pk.pk.EGFP}
Cytology
Description

The FRT cassette has been excised, allowing the Act5C promoter to drive expression of the pk isoform of pk tagged with EGFP.

(Strutt et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
Act5C
Encoded product / tool
pk
Tagged with
EGFP
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of pkAct5C.pk.T:Avic\GFP-EGFP produces eye planar polarity phenotypes. The ommatidia in the eye adopt random chirality.

      (Strutt et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      pkAct5C.pk.EGFP rescues pk1

      (Strutt et al., 2013)
      Partially rescues

      pkAct5C.pk.EGFP partially rescues pkpk-sple-13

      (Strutt et al., 2013)
      Comments

      Expression of pkAct5C.pk.T:Avic\GFP-EGFP fully rescues the wing planar polarity phenotype seen in pkpk-sple-13 mutant wings.

      Expression of pkAct5C.pk.T:Avic\GFP-EGFP partially rescues the eye planar polarity phenotype seen in pkpk-sple-13 mutant flies. The misrotation is rescued, but the chirality defects are still present.

      Expression of pkAct5C.pk.T:Avic\GFP-EGFP fully rescues the wing planar polarity phenotype seen in pk1 mutant wings.

      (Strutt et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      pkAct5C.pk.EGFP
      pkAct5C.pk.T:Avic\GFP-EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (3)