FB2024_03 , released June 25, 2024
Allele: Dmel\Adf1S64A.S184A.UAS.Tag:FLAG
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General Information
Symbol
Dmel\Adf1S64A.S184A.UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0288416
Feature type
allele
Associated gene
Dmel\Adf1
Associated Insertion(s)
Carried in Construct
P{UAS-Adf1.S64A.S184A}
Key Links
Transgenic product class
missense_variant
(Timmerman et al., 2013)
polypeptide_post_translational_processing_variant
(Timmerman et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Timmerman et al., 2013)
polypeptide_post_translational_processing_variant
(Timmerman et al., 2013)
Mutagen
in vitro construct
(Timmerman et al., 2013)
Progenitor genotype
Carried in construct
P{UAS-Adf1.S64A.S184A}
Cytology
Description

UAS regulatory sequences drive expression of a phospho-null form of Adf1 containing the amino acid replacements S64A and S184A. The protein is tagged with Tag:FLAG.

(Timmerman et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Adf1
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Adf1S64A.S184A.Scer\UAS.T:Zzzz\FLAG in the RP2 motor neurons (using the Scer\GAL4eve.RRK driver to drive expression of Scer\FLP1Scer\UAS.cUa which then induces clones of cells expressing Scer\GAL4Act.PU) results in a dramatic decrease in dendritic complexity, number of dendritic branches and total dendrite length compared to controls.

      The amplitude of the excitatory junctional potential (WWJP) and the miniature EJP are normal in animals expressing Adf1S64A.S184A.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4futsch-C380. mEJP frequency and quantal content are also unaffected. 50% of the animals fail to display motor neuron firing following stimulation of contralateral sensory afferents at 10Hz for 1.5 seconds. This defect is still seen if Scer\GAL80Cha.PK is also present.

      Larvae expressing Adf1S64A.S184A.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4futsch-C380 show reduced locomotion compared to controls.

      Weak expression of Adf1S64A.S184A.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4futsch-C380 results in adults which show prominent defects in the Buridan assay. These defects are still seen if expression is limited to the adult stage using Scer\GAL80ts.αTub84B.

      (Timmerman et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Fas2dsRNA.Scer\UAS.A partially rescues the reduction in dendritic complexity, number of dendritic branches and total dendrite length seen in RP2 motor neurons expressing Adf1S64A.S184A.Scer\UAS.T:Zzzz\FLAG (using the Scer\GAL4eve.RRK driver to drive expression of Scer\FLP1Scer\UAS.cUa which then induces clones of cells expressing Scer\GAL4Act.PU).

      Co-expression of stauJF01764 partially rescues the dendritic growth defects seen in RP2 motor neurons expressing Adf1S64A.S184A.Scer\UAS.T:Zzzz\FLAG (using the Scer\GAL4eve.RRK driver to drive expression of Scer\FLP1Scer\UAS.cUa which then induces clones of cells expressing Scer\GAL4Act.PU).

      (Timmerman et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Adf-1[S64/184A]
      (Timmerman et al., 2013)
      Adf1S64A.S184A.Scer\UAS.T:Zzzz\FLAG
      Adf1S64A.S184A.UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (1)