FB2024_03 , released June 25, 2024
Allele: Ecol\lexAGMR18H11
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General Information
Symbol
Ecol\lexAGMR18H11
Species
E. coli
Name
FlyBase ID
FBal0289019
Feature type
allele
Associated gene
Ecol\lexA
Associated Insertion(s)
Carried in Construct
P{GMR18H11-lexA}
Also Known As
R18H11-lexA
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Pfeiffer et al., 2013.10.9)
Progenitor genotype
Carried in construct
P{GMR18H11-lexA}
Cytology
Description

A 3354 base pair fragment from the flanking non-coding or intronic region of pdfr is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.

(Pfeiffer et al., 2013.10.9)
Allele components
Component
Use(s)
Regulatory region(s)
GMR18H11
Encoded product / tool
lexA::p65
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      DN1 neuron | subset
      (Sun et al., 2022, Guo et al., 2016)
      DN1p neuron
      (Jin et al., 2021)
      Additional Information
      Statement
      Reference

      EcollexAGMR18H11 drives expression in a subset of DN1 neurons in the adult dorsal brain; this subset partially overlaps the DN1 neurons labeled by ScerGAL4Clk.4.1M. These neurons coexpress ScerGAL4VGlut-MI04979-TG4.2.

      (Guo et al., 2016)
       
      Marker for
      DN1 neuron
      (Sun et al., 2022)
      Reflects expression of
      Pdfr
      (Guo et al., 2016)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.20xUAS.FRT.Venus with Scer\FLP1L.8xlexAop and 627 nm (red) light) of cells expressing both Scer\GAL4Clk.2341 and Ecol\lexAGMR18H11 in adults significantly increases sleep, compared to controls; when expression is restricted to a subset of Scer\GAL4Clk.2341 and Ecol\lexAGMR18H11 cells (using Scer\GAL80cry.PS) sleep is significantly reduced, compared to controls.

      Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.13XLexAop2.Venus with 627 nm (red) light) of Ecol\lexAGMR18H11 cells in adults significantly increases sleep, and significantly reduces evening, but not morning, activity, despite periodic mechanical stimulation, compared to controls; activation does not significantly affect the initial startle response following mechanical stimulation, but total movement after stimulation is significantly reduced, compared to controls.

      (Guo et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Although alleles from the Janelia Farm lexA driver collection incorporate the same enhancer fragments used to create the Janelia Farm GAL4 driver alleles (GMR_Brain_exp_1), significant differences are frequently observed in the expression pattern of a given enhancer fragment inserted in the P{CaryP}attP40 or other docking site compared to the same fragment inserted in the P{CaryP}attP2 docking site. Generally the Ecol\lexA patterns are subsets of the Scer\GAL4 patterns.

      (Pfeiffer et al., 2013.10.9)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Ecol\lexAGMR18H11
      Name Synonyms
      Secondary FlyBase IDs
        References (9)