FlyBase Allele Report: Scer\GAL4[Clk.-856]

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Allele: Scer\GAL4^Clk.-856

General Information

Symbol

Scer\GAL4^Clk.-856

Species

S. cerevisiae

Name

FlyBase ID

FBal0264191

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{Clk-GAL4.-856}

Also Known As

Clk856-gal4, Clk⁸⁵⁶-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Gummadova et al., 2009)

Progenitor genotype

Carried in construct

P{Clk-GAL4.-856}

Cytology

Description

A Clk fragment from -856 to +1485 (amino acid residues 1-18) is placed upstream of sequence encoding a GAL4 driver.

(Gummadova et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

LN period neuron

(Gummadova et al., 2009)

adult DN period neuron

(Gummadova et al., 2009)

adult LP neuron | faint

(Gummadova et al., 2009)

adult s-LNv neuron

(Fujiwara et al., 2018)

(Fujiwara et al., 2018)

Additional Information

Statement

Reference

Drives expression in adult pacemaker neurons, including adult DN period neurons, LN period neurons, LP neurons, s-LNv neurons and l-Lnv neurons.

(FlyBase Curators, 2017)

Marker for

Reflects expression of

(Gummadova et al., 2009)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm | adult stage, with alka^JF02149

(Frenkel et al., 2017)

abnormal circadian rhythm | adult stage, with Grd^GD2702

(Frenkel et al., 2017)

abnormal locomotor behavior | adult stage, with Dop1R1^HM04077

(Fernandez-Chiappe et al., 2020)

abnormal locomotor behavior | adult stage, with Dop1R2^JF02043

(Fernandez-Chiappe et al., 2020)

abnormal locomotor rhythm | adult stage, with CCHa1^KK112676

(Fujiwara et al., 2018)

abnormal locomotor rhythm | adult stage, with cyc^{Δ.UAS.Tag:MYC}

(Bulthuis et al., 2019)

abnormal locomotor rhythm | adult stage, with fray^GL00704

(Eick et al., 2022)

abnormal locomotor rhythm | adult stage, with fray^HMC04151

(Eick et al., 2022)

abnormal locomotor rhythm | adult stage, with fray^HMJ02228

(Eick et al., 2022)

abnormal locomotor rhythm | adult stage, with fray^HMS01794

(Eick et al., 2022)

abnormal locomotor rhythm | adult stage, with kcc^GD3565

(Eick et al., 2022)

abnormal locomotor rhythm | adult stage, with kcc^HMS01058

(Eick et al., 2022)

abnormal locomotor rhythm | adult stage, with kcc^KK107965

(Eick et al., 2022)

abnormal locomotor rhythm | adult stage, with kcc^UAS.B.cEa

(Eick et al., 2022)

abnormal locomotor rhythm | adult stage, with kcc^UAS.D.cEa

(Eick et al., 2022)

abnormal locomotor rhythm | adult stage, with NKCC^{UAS.4xgRNA.pCFD6}, Spyo\Cas9^UAS.P2

(Eick et al., 2022)

abnormal locomotor rhythm | adult stage, with Scer\GAL80^Pdf.PS, cyc^{Δ.UAS.Tag:MYC}

(Bulthuis et al., 2019)

abnormal locomotor rhythm | adult stage, with Spyo\Cas9^UAS.P2, kcc^{UAS.3xgRNA.pCFD6}

(Eick et al., 2022)

abnormal locomotor rhythm | adult stage, with Spyo\Cas9^UAS.P2, qsm^{UAS.4xgRNA.pCFD6}

(Eick et al., 2022)

abnormal locomotor rhythm | adult stage, with Wnk^GD12160

(Eick et al., 2022)

abnormal locomotor rhythm | adult stage, with Wnk^KK102654

(Eick et al., 2022)

abnormal sleep | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.20xUAS.FRT.Venus}, Ecol\lexA^GMR12G04, Scer\FLP1^L.8xlexAop

(Guo et al., 2018)

abnormal sleep | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.20xUAS.FRT.Venus}, Ecol\lexA^GMR18H11, Scer\FLP1^L.8xlexAop

(Guo et al., 2018)

abnormal sleep | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.20xUAS.FRT.Venus}, Ecol\lexA^GMR18H11, Scer\FLP1^L.8xlexAop, Scer\GAL80^cry.PS

(Guo et al., 2018)

abnormal sleep | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.20xUAS.FRT.Venus}, Ecol\lexA^GMR19C05, Scer\FLP1^L.8xlexAop

(Guo et al., 2018)

abnormal sleep | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.20xUAS.FRT.Venus}, Ecol\lexA^GMR43D05, Scer\FLP1^L.8xlexAop

(Guo et al., 2018)

abnormal sleep | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.20xUAS.FRT.Venus}, Ecol\lexA^GMR65D05, Scer\FLP1^L.8xlexAop

(Guo et al., 2018)

abnormal sleep | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.20xUAS.FRT.Venus}, Ecol\lexA^GMR66B05, Scer\FLP1^L.8xlexAop

(Guo et al., 2018)

abnormal sleep | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.20xUAS.FRT.Venus}, Ecol\lexA^GMR67F03, Scer\FLP1^L.8xlexAop

(Guo et al., 2018)

abnormal sleep | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.20xUAS.FRT.Venus}, Ecol\lexA^GMR77H08, Scer\FLP1^L.8xlexAop

(Guo et al., 2018)

Phenotype Manifest In

Detailed Description

Statement

Reference

Elimination of molecular clock function (using cyc^{Δ.UAS.Tag:MYC}) in Scer\GAL4^Clk.2341 cells in adults significantly reduces the strength of the circadian rhythm of sleep-wake cycles, compared to controls; this is also the case when expression is restricted to a smaller subset of cells using Scer\GAL80^Pdf.PS.

Inhibition (using Hsap\KCNJ2^UAS.EGFP) of Scer\GAL4^Clk.2341 cells significantly reduces the strength of the circadian rhythm of sleep-wake cycles, compared to controls; this is also the case when inhibition is restricted to a smaller subset of cells using Scer\GAL80^Pdf.PS and when inhibition of this subset is restricted to the adult stage using a Gal80[ts] transgene.

(Bulthuis et al., 2019)

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.20xUAS.FRT.Venus} with Scer\FLP1^L.8xlexAop and 627 nm (red) light) of cells expressing Scer\GAL4^Clk.2341 with Ecol\lexA^GMR19C05 or Ecol\lexA^GMR43D05 in adults significantly reduces sleep, compared to controls; activation of cells expressing Scer\GAL4^Clk.2341 with Ecol\lexA^GMR73B06, Ecol\lexA^GMR64A11, Ecol\lexA^GMR81E08, Ecol\lexA^GMR55C09, Ecol\lexA^GMR19A11, Ecol\lexA^GMR70E02 or Ecol\lexA^GMR70G01 in adults does not significantly affect sleep, compared to controls; activation of cells expressing Scer\GAL4^Clk.2341 with Ecol\lexA^GMR66B05, Ecol\lexA^GMR77H08, Ecol\lexA^GMR12G04, Ecol\lexA^GMR67F03, Ecol\lexA^GMR65D05 or Ecol\lexA^GMR18H11 in adults significantly increases sleep, compared to controls; activation of a subset of Scer\GAL4^Clk.2341 and Ecol\lexA^GMR16C05-expressing cells (restricted using Scer\GAL80^cry.PS) in adults does not significantly affect sleep, compared to controls; activation of a subset of Scer\GAL4^Clk.2341 and Ecol\lexA^GMR18H11-expressing cells (restricted using Scer\GAL80^cry.PS) in adults significantly reduces sleep, compared to controls.

(Guo et al., 2018)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Clk.-856, alka^JF02149 has abnormal circadian rhythm | adult stage phenotype, enhanceable by SecCl^JF02437/Scer\GAL4^Clk.-856/Grd^GD2702

(Frenkel et al., 2017)

Enhancer of

Statement

Reference

SecCl^JF02437, Scer\GAL4^Clk.-856, Grd^GD2702 is an enhancer of abnormal circadian rhythm | adult stage phenotype of Scer\GAL4^Clk.-856, alka^JF02149

(Frenkel et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

93198

w^*; P{Clk-GAL4.-856}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^Clk.-856

Scer\GAL4^Clk.2341

Name Synonyms

Secondary FlyBase IDs

References (39)

Report Sections