FlyBase Allele Report: Dmel\cyc[Δ.UAS.Tag:MYC]

General Information

Symbol

Dmel\cyc^{Δ.UAS.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0159371

Feature type

allele

Associated gene

Dmel\cyc

Associated Insertion(s)

Carried in Construct

P{UAS-cyc.Δ}

Also Known As

UAS-cycΔ, UAS-cyc^DN

Key Links

Transgenic product class

dominant_negative_variant

(Tanoue et al., 2004)

inframe_deletion

(Tanoue et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Tanoue et al., 2004)

inframe_deletion

(Tanoue et al., 2004)

Mutagen

in vitro construct

(Tanoue et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-cyc.Δ}

Cytology

Description

UASt regulatory sequences drive expression of a mutant form of cyc which lacks the basic region (codons 31-45) and is tagged with Tag:MYC. Expected to produce a dominant-negative form of cyc.

(Tanoue et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

cyc

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm, with Scer\GAL4^Or22a.8197

(Tanoue et al., 2004)

abnormal circadian rhythm, with Scer\GAL4^{Orco.2.642.Hsim\VP22}

(Tanoue et al., 2004)

abnormal circadian rhythm, with Scer\GAL4^tim.PE

(Tanoue et al., 2004)

abnormal eclosion rhythm, with Scer\GAL4^P2.4.Pdf

(Selcho et al., 2017)

abnormal eclosion rhythm, with Scer\GAL4^phtm.PO

(Selcho et al., 2017)

abnormal eclosion rhythm, with Scer\GAL4^tim.PE

(Selcho et al., 2017)

abnormal eclosion rhythm, with Scer\GAL4^tim.PE, Scer\GAL80^phtm.1.2

(Selcho et al., 2017)

abnormal locomotor rhythm, with Scer\GAL4^P2.4.Pdf

(Tanoue et al., 2004)

abnormal locomotor rhythm, with Scer\GAL4^tim.PE

(Sehadova et al., 2009, Tanoue et al., 2004)

abnormal locomotor rhythm | adult stage, with Scer\GAL4^Clk.-856

(Bulthuis et al., 2019)

abnormal locomotor rhythm | adult stage, with Scer\GAL4^Clk.-856, Scer\GAL80^Pdf.PS

(Bulthuis et al., 2019)

abnormal locomotor rhythm | adult stage, with Scer\GAL4^Dvir\Pdf.1.9, Scer\GAL4^GMR78G02, Scer\GAL80^Pdf.PS

(Bulthuis et al., 2019)

abnormal locomotor rhythm | adult stage, with Scer\GAL4^P2.4.Pdf

(Bulthuis et al., 2019)

abnormal locomotor rhythm | adult stage, with Scer\GAL4^tim.UAS

(Cavey et al., 2016)

abnormal mating rhythm | male, with Scer\GAL4^Desat1.PB

(Krupp et al., 2013)

abnormal sleep, with Scer\GAL4^P2.4.Pdf

(Liu et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

Flies expressing cyc^{Δ.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^P2.4.Pdf show a significant increase in sleep latency compared to controls.

(Liu et al., 2014)

Males expressing cyc^{Δ.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^desat1.PB show a significant increase in the levels of (z)-7-Tricosene and (z)-7-Pentacosene compared to wild type. These males show a temporal difference in remating behaviour compared to wild type, without affecting the total number of matings.

(Krupp et al., 2013)

Flies expressing cyc^{Δ.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^tim.PE show abnormal temperature entrainment of behavioural rhythms.

Flies expressing cyc^{Δ.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^nan.PK normal temperature entrainment of behavioural rhythms.

(Sehadova et al., 2009)

Flies expressing cyc^{Δ.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^tim.PE are arrhythmic with respect to locomotor activity when assayed for at least 5 days in constant darkness after being entrained to 12 hour light:12 hour dark cycles for 3 days, in contrast to controls which show robust locomotor activity rhythms under these conditions. Only 21% of flies expressing cyc^{Δ.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^P2.4.Pdf are arrhythmic with respect to locomotor activity when assayed for at least 5 days in constant darkness after being entrained to 12 hour light:12 hour dark cycles for 3 days, in contrast to controls which show robust locomotor activity rhythms under these conditions. The diurnal changes in the mean electroantennogram (EAG) response to ethyl acetate which are seen in wild-type flies maintained in 12 hour light:12 hour dark cycles are abolished in flies expressing cyc^{Δ.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^tim.PE or Scer\GAL4^{Or83b.2.642.T:Hsim\VP22}. The diurnal changes in the mean electroantennogram (EAG) response to ethyl acetate which are seen in wild-type flies maintained in 12 hour light:12 hour dark cycles are abolished in flies expressing cyc^{Δ.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Or22a.8197. However, if the EAG recordings are taken from a more lateral position on the antenna (which is outside the region of Scer\GAL4^Or22a.8197 expression), then the EAG responses are rhythmic, albeit at a lower amplitude.

(Tanoue et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

600602

w^*; P{UAS-cyc.Δ}1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

cyc^{Δ.Scer\UAS.T:Hsap\MYC}

cyc^{Δ.UAS.Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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