FB2024_03 , released June 25, 2024
Allele: Tn\neoRUAS.GFP
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General Information
Symbol
Tn\neoRUAS.GFP
Species
b. transposon
Name
FlyBase ID
FBal0291617
Feature type
allele
Associated gene
Tn\neoR
Associated Insertion(s)
Carried in Construct
P{UAS-neo.GFP}
Transgenic product class
wild_type
(Stern et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Stern et al., 2012)
Mutagen
in vitro construct
(Stern et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-neo.GFP}
Cytology
Description

UAS regulatory sequences drive expression of Tn\neoR which is tagged with GFP.

(Stern et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Tn\neoR
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Tn\neoRScer\UAS.T:Avic\GFP under the control of one of Scer\GAL4h.PU, Scer\GAL4drm.7.1, Scer\GAL4hs.PU, Scer\GAL4wg.PU, Scer\GAL4ptc.PU, Scer\GAL4elav.PU, Scer\GAL4dpp.PU or Scer\GAL4ftz.ng allows significantly higher adult survival in the presence of a concentration of G418 which is lethal to wild-type controls. Expression of Tn\neoRScer\UAS.T:Avic\GFP under the control of Scer\GAL4ey.PU does not result in survival under these conditions.

      Animals expressing Tn\neoRScer\UAS.T:Avic\GFP under the control of Scer\GAL4drm.7.1 and challenged with G418 develop into significantly smaller adults than control unchallenged animals.

      Animals expressing Tn\neoRScer\UAS.T:Avic\GFP under the control of Scer\GAL4hs.PU and challenged with G418 sometimes develop into adults with wing abnormalities.

      Expression of Tn\neoRScer\UAS.T:Avic\GFP under the control of Scer\GAL4Act5C.PU results in 100% adult survival in the presence of a concentration of G418 which is lethal to wild-type controls.

      Expression of Tn\neoRScer\UAS.T:Avic\GFP under the control ofScer\GAL4h.PU results in 100% adult survival in the presence of a concentration of G418 which is lethal to wild-type controls. The challenged larvae show a developmental delay of approximately 2-3 days under these conditions.

      The developmental delay which is seen in animals expressing Tn\neoRScer\UAS.T:Avic\GFP under the control ofScer\GAL4h.PU and exposed to G418 during their development is retained in several generations of progeny of these animals grown for multiple generations in a G418-free environment, before reverting to the normal phenotype. A heritable delay in development is also seen if expression is driven using Scer\GAL4drm.7.1.

      (Stern et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      The ability of animals expressing Tn\neoRScer\UAS.T:Avic\GFP under the control of Scer\GAL4h.PU to survive in the presence of a concentration of G418 which is lethal to wild-type controls is partially suppressed if they also express Pchs.PF (using heat shock).

      The ability of animals expressing Tn\neoRScer\UAS.T:Avic\GFP under the control of Scer\GAL4h.PU to survive in the presence of a concentration of G418 which is lethal to wild-type controls is partially suppressed if they are also heterozygous for bsk1.

      Co-expression of phoVDRC.cUa enhances the survival of animals expressing Tn\neoRScer\UAS.T:Avic\GFP under the control of Scer\GAL4drm.7.1 in the presence of a concentration of G418 which is lethal to wild-type controls.

      (Stern et al., 2012)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Tn\neoRScer\UAS.T:Avic\GFP
      Tn\neoRUAS.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)