FB2024_03 , released June 25, 2024
Allele: Dmel\Rpn11RNAi.UAS
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General Information
Symbol
Dmel\Rpn11RNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0294983
Feature type
allele
Associated gene
Dmel\Rpn11
Associated Insertion(s)
Carried in Construct
P{UAS-Rpn11.RNAi}
Key Links
Transgenic product class
RNAi_reagent
(Tonoki et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Tonoki et al., 2009)
Mutagen
in vitro construct
(Tonoki et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-Rpn11.RNAi}
Cytology
Description

UAS regulatory sequences drive expression of an inverted repeat fragment of Rpn11 coding sequence.

(Tonoki et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Rpn11
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  neurodegenerative disease
      modeled by Hsap\ATXN3tr.Q78.UAS.Tag:HA
      (Tonoki et al., 2009)
      modeled by Hsap\HTTGMR.Q120
      (Tonoki et al., 2009)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Rpn11dsRNA.Scer\UAS under the control of Scer\GAL4da.G32 in adult flies (using the temperature sensitive Scer\GAL80ts.αTub84B allele to limit expression) results in a severe reduction in mean lifespan.

      Expression of Rpn11dsRNA.Scer\UAS under the control of Scer\GAL4GMR.PF results in a progressive neurodegeneration phenotype in the eye.

      (Tonoki et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of Rpn11dsRNA.Scer\UAS enhances the progressive eye degeneration phenotype caused by expression of Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF.

      Co-expression of Rpn11dsRNA.Scer\UAS (under the control of Scer\GAL4GMR.PF) enhances the age-related rhabdomeric loss seen in flies expressing Hsap\HTTGMR.Q120.

      Polyglutamine aggregation is significantly enhanced when Rpn11dsRNA.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF in Hsap\HTTGMR.Q120 flies.

      (Tonoki et al., 2009)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Rpn11RNAi.UAS
      Rpn11dsRNA.Scer\UAS
      Rpn11dsRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)