FlyBase Allele Report: Dmel\Kat60[UAS.cMa]

General Information

Symbol

Dmel\Kat60^UAS.cMa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Mao

FlyBase ID

FBal0295262

Feature type

allele

Associated gene

Dmel\Kat60

Associated Insertion(s)

Carried in Construct

M{UAS-Kat60.M}

P{UAS-Kat60.M}

Also Known As

UAS-kat60, UAS-Kat-60

Key Links

Transgenic product class

cDNA

(Mao et al., 2014)

wild_type

(Mao et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Mao et al., 2014)

wild_type

(Mao et al., 2014)

Mutagen

in vitro construct

(Mao et al., 2014)

Progenitor genotype

Carried in construct

M{UAS-Kat60.M}

P{UAS-Kat60.M}

Cytology

Description

UASt regulatory sequences drive expression of a full-length Kat60 cDNA.

(Mao et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Kat60

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dendritic tree, with Scer\GAL4^ppk.PG

(Mao et al., 2014)

embryonic/larval sensory neuron | larval stage, with Scer\GAL4^ppk.PU

(Bu et al., 2021)

epithelial cell of wing disc | P-stage, with Scer\GAL4^ap-md544

(Tran et al., 2024)

larval multidendritic class IV neuron, with Scer\GAL4^ppk.PG

(Mao et al., 2014)

microtubule, with Scer\GAL4^C57

(Mao et al., 2014)

microtubule | P-stage, with Scer\GAL4^ap-md544

(Tran et al., 2024)

NMJ bouton | third instar larval stage, with Scer\GAL4^C57

(Mao et al., 2014)

NMJ bouton | third instar larval stage, with Scer\GAL4^elav.PU

(Mao et al., 2014)

wing, with Scer\GAL4^ap-md544

(Tran et al., 2024)

Detailed Description

Statement

Reference

Expression of Kat60^Scer\UAS.cMa under the control of either Scer\GAL4^elav.PU or Scer\GAL4^C57 results in a significant increase in the number of satellite boutons at the neuromuscular junction in third instar larvae compared to controls (expression driven by Scer\GAL4^elav.PU results in a greater increase).

Expression of Kat60^Scer\UAS.cMa under the control of Scer\GAL4^ppk.PG results in altered dendritic elaboration in the class IV dendritic arborization neurons: the total dendritic length, the number of dendritic termini and the total dendrite field area are significantly reduced compared to wild type.

Expression of Kat60^Scer\UAS.cMa under the control of Scer\GAL4^C57 results in a dramatic decrease in perinuclear microtubule density in larval muscle cells, with sparser and shorter microtubule fibres compared to wild type.

(Mao et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Kat60^UAS.cMa, Scer\GAL4^elav.PU has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by HDAC6^UAS.cDa, Scer\GAL4^elav.PU

(Mao et al., 2014)

Kat60^UAS.cMa, Scer\GAL4^C57 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by HDAC6^UAS.cDa, Scer\GAL4^C57

(Mao et al., 2014)

Kat60^UAS.cMa, Scer\GAL4^ppk.PG has abnormal neuroanatomy phenotype, suppressible by HDAC6^UAS.cDa, Scer\GAL4^ppk.PG

(Mao et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Kat60^UAS.cMa, Scer\GAL4^elav.PU has NMJ bouton | third instar larval stage phenotype, suppressible by HDAC6^UAS.cDa, Scer\GAL4^elav.PU

(Mao et al., 2014)

Kat60^UAS.cMa, Scer\GAL4^C57 has NMJ bouton | third instar larval stage phenotype, suppressible by HDAC6^UAS.cDa, Scer\GAL4^C57

(Mao et al., 2014)

Kat60^UAS.cMa, Scer\GAL4^ppk.PG has larval multidendritic class IV neuron phenotype, suppressible by HDAC6^UAS.cDa, Scer\GAL4^ppk.PG

(Mao et al., 2014)

Kat60^UAS.cMa, Scer\GAL4^ppk.PG has dendritic tree phenotype, suppressible by HDAC6^UAS.cDa, Scer\GAL4^ppk.PG

(Mao et al., 2014)

Kat60^UAS.cMa, Scer\GAL4^C57 has microtubule phenotype, suppressible by HDAC6^UAS.cDa, Scer\GAL4^C57

(Mao et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Tat^KO has no effect on Scer\GAL4^ppk.PG>Kat60^UAS.cMa-induced dendrite arbor remodeling in larval C4da neurons.

(Yan et al., 2018)

Co-expression of HDAC6^Scer\UAS.cDa completely rescues the increase in satellite boutons at the neuromuscular junction seen in third instar larvae expressing Kat60^Scer\UAS.cMa under the control of either Scer\GAL4^elav.PU or Scer\GAL4^C57.

Co-expression of HDAC6^Scer\UAS.cDa does not rescue the defects in dendritic elaboration in the class IV dendritic arborization neurons which are caused by expression of Kat60^Scer\UAS.cMa under the control of Scer\GAL4^ppk.PG.

Co-expression of HDAC6^Scer\UAS.cDa rescues the decrease in perinuclear microtubule density which is seen in larval muscle cells expressing Kat60^Scer\UAS.cMa under the control of Scer\GAL4^C57.

The decrease in perinuclear microtubule density which is seen in larval muscle cells expressing Kat60^Scer\UAS.cMa under the control of Scer\GAL4^C57 is not altered by the presence of HDAC6^KO. The microtubule fibres are significantly shorter in the double mutant cells.

(Mao et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Kat60^UAS.cMa rescues Kat60^17A

(Mao et al., 2014)

Kat60^UAS.cMa/Scer\GAL4^da.PU rescues Kat60^17A

(Mao et al., 2014)

Kat60^UAS.cMa/Scer\GAL4^elav.PU rescues Kat60^17A

(Mao et al., 2014)

Comments

The M{UAS-Kat60.M}ZH-51D insertion line effectively rescues the lethality of Kat60^17A animals, even in the absence of a Scer\GAL4 driver. The anatomical and electrophysiological defects at the neuromuscular junction which are seen in Kat60^17A third instar larvae are rescued by two copies of Kat60^Scer\UAS.cMa.

(Mao et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

64117

w^*; M{UAS-Kat60.M}ZH-51D/CyO

64115

y¹ w^*; wg^Sp-1/CyO; P{UAS-Kat60.M}1-1

Notes on Origin

Discoverer