FlyBase Allele Report: Dmel\Clk[Tag:V5.WT]

General Information

Symbol

Dmel\Clk^Tag:V5.WT

Species

D. melanogaster

Name

FlyBase ID

FBal0297401

Feature type

allele

Associated gene

Dmel\Clk

Associated Insertion(s)

Carried in Construct

P{Clk.WT.V5}

Also Known As

p{dClk-WT}, ClkWT

Key Links

Genomic Maps

JBrowse

Transgenic product class

genomic_DNA

(Mahesh et al., 2014)

wild_type

(Mahesh et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Mahesh et al., 2014)

wild_type

(Mahesh et al., 2014)

Mutagen

in vitro construct

(Mahesh et al., 2014)

Progenitor genotype

Carried in construct

P{Clk.WT.V5}

Cytology

Description

A genomic fragment containing 15.5kb beginning ~8kb upstream of the Clk translation start and ending ~2.5kb downstream of the Clk stop codon. The construct is tagged at the C-terminal end with Tag:V5.

(Mahesh et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

Clk

Encoded product / tool

Clk

Tagged with

Tag:V5

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

rescue_region

3L:7,761,751..7,778,098 [-]

Comment:

Approximate extent of Clk rescue fragment inferred from primer sequences.

(Mahesh et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm | adult stage, with Clk^out

(Lee et al., 2016)

abnormal locomotor rhythm

(Lerner et al., 2015, Lee et al., 2014)

abnormal temperature response

(Lee et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

Clk^out homozygotes bearing Clk^Tag:V5.WT that are kept under constant darkness and subject to 12h:12h 24[o] C:29 [o]C temperature cycles exhibit bimodal peaks of activity with an anticipatory activity increase before temperature transitions; upon transfer to constant 24[o]C, these individuals maintain some rhythmicity and manifest a 23.1h period.

(Lee et al., 2016)

A slightly shortened circadian period and little effect on the overall rhythm strength is observed in flies with one extra copy of Clk^T:SV5\V5.WT in addition to the endogenous Clk.

(Lerner et al., 2015)

Expression of Clk^T:SV5\V5.WT results in a shortening of the circadian period compared to wild type controls . Flies also exhibit defects in behavioural rhythms seen in wild type flies in response to daily temperature cycles (in the absence of light).

(Lee et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Clk^out/Clk^Tag:V5.WT is a non-enhancer of abnormal locomotor rhythm phenotype of nmo^P1/Df(3L)Exel6279

(Mahesh et al., 2014)

NOT Suppressor of

Statement

Reference

Clk^out/Clk^Tag:V5.WT is a non-suppressor of abnormal locomotor rhythm phenotype of nmo^P1/Df(3L)Exel6279

(Mahesh et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Clk^T:SV5\V5.WT (in a Clk^out mutant background) does not alter the locomotor rhythm defects seen in nmo^P1/Df(3L)Exel6279.

(Mahesh et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Clk^Tag:V5.WT rescues Clk^out

(Lee et al., 2016)

Clk^Tag:V5.WT rescues Clk^out

(Lee et al., 2014)

Clk^Tag:V5.WT rescues Clk^out

(Mahesh et al., 2014)

Comments

Clk^Tag:V5.WT rescues the circadian rhythm defects observed in Clk^out homozygous adults.

(Lee et al., 2016)

Expression of Clk^T:SV5\V5.WT rescues the behavioral rhythm defects seen in Clk^out following following exposure to entrainment conditions (12 hours light: 12 hours dark followed by several days in constant darkness). A similar rescue is seen at 18, 25 and 29[o]C. The failure of Clk^out mutant flies to entrain to daily temperature cycles in the absence of light is also rescued in these flies.

(Lee et al., 2014)

Expression of Clk^T:SV5\V5.WT rescues the locomotor activity rhythm defects in >90% of Clk^out flies with periods of ~24.2 hours.

(Mahesh et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Clk^T:SV5\V5.WT

Clk^Tag:V5.WT

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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