FlyBase Allele Report: Dmel\nmo[P1]

Flies homozygous for nmo^P1 show ommatidia arrested at around 45[o] of rotation. The rate of rotation appears to be lower in these mutants compared to wild-type.

nmo^P1 mutant flies exhibit wings that are smaller than wild-type and display a more rounded morphology and a held-out phenotype. Eyes are externally rough and narrower than wild-type. Tangential sections of those eyes reveal a high number of under-rotated ommatidia with a mean orientation angle of 59.3[o] with respect to the equator.

nmo^P1 mutant eye discs display ommatidial rotation that is slower than in wild-type and that stop rotating prematurely. Apical shape change in the interommatidial cells, along with changes in cell area, are less evident in nmo^P1 discs than in controls. The cone cell precursors also display abnormal behavior, with these cells being significantly more static in mutants than in controls.

(Muñoz-Soriano et al., 2013)

Mutant ommatidia show reduced rotation of ommatidia compared to controls.

(Mirkovic et al., 2011)

nmo^P1/Df(3L)Exel6279 adults exhibit shorter circadian period of locomotor activity rhythms under constant darkness compared to controls.

(Yu et al., 2011)

Most neuromuscular junctions (NMJs) at muscles 6 and 7 lack branches containing more than five boutons in nmo^P1/Df(3L)Exel6279 third instar larvae. There is a strong reduction in the number of type I synaptic boutons at muscles 6 and 7 and in the number of boutons at muscle 4 NMJs in the mutant larvae compared to wild type.

The number of boutons per muscle surface area is reduced at the neuromuscular junction in nmo^P1/Df(3L)Exel6279, nmo^P1/Df(3L)pbl-X1 and nmo^DB24/nmo^P1 third instar larvae.

(Merino et al., 2009)

Mutant ommatidial precursors rotate at a slower rate than wild-type ommatidial precursors in the developing eye. Initiation of ommatidial rotation and the first approximately 7[o] of rotation occurs normally in the mutant eye disc, but the average angle of rotation is significantly lower in the mutant eye discs than in wild-type from ommatidial row 5 onwards. The mutant ommatidia stall at 45[o] of rotation in the mutant eye disc, although in adults the average angle of rotation is 61[o] +/- 23 in mutant eyes.

(Fiehler and Wolff, 2008)

No ectopic wing margin bristles are seen in nmo^P1 mutant wings.

(Wu et al., 2008)

Ommatidia are arrested at approximately 45^o to the equator.

(Brown and Freeman, 2003)

In larval eye discs of nmo^j147-1/nmo^P1, ommatidial rotation is arrested at about 45^o, instead of 60^o-65^o as in wild-type.

(Chou and Chien, 2002)

nmo^P1 heterozygous flies (genetic background = Canton-S) have a small but significant decrease in geotaxis score compared to wild-type (Canton-S) flies.

(Toma et al., 2002)

nmo^P1/nmo^adk2 flies have a smaller eye than normal in which the ommatidia are square instead of the normal hexagonal shape and are generally flanked by 4 interommatidial bristles (instead of the usual 3 bristles at alternating corners). Slight extra vein material near the posterior crossvein is seen in a small percentage of nmo^P1 flies. Flies do not show any noticeable polarity defects in the wing.

(Verheyen et al., 2001)

Eyes of nmo mutant flies are slightly narrowed anterior-posterior, and the facets are square rather than hexagonal. Pigment cell lattice is abnormal, especially at the corners, which have disarranged bristle and secondary pigment cells. Occasionally ommatidia are fused three to ten per eye. Most ommatidia have normal numbers of photoreceptor cells, although some lack a few. Viability is reduced to 10% of wild type and the angle of wings is 15^o to 30^o. In mutant eye discs the 5 cell precluster completes its first 45^o rotation, but does not continue to the full 90^o rotation. This also applies to the cone cells and primary pigment cells.

(Choi and Benzer, 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

nmo^P1/Df(3L)Exel6279 has abnormal locomotor rhythm phenotype, enhanceable by Clk^S859A.Tag:V5/Clk^out

(Mahesh et al., 2014)

nmo^P1 has abnormal planar polarity phenotype, enhanceable by Vang^stbm-6

(Mirkovic et al., 2011)

nmo^P1 has abnormal planar polarity phenotype, enhanceable by pk^sple-1

(Mirkovic et al., 2011)

NOT Enhanced by

Statement

Reference

nmo^P1/Df(3L)Exel6279 has abnormal locomotor rhythm phenotype, non-enhanceable by Clk^out/Clk^Tag:V5.WT

(Mahesh et al., 2014)

nmo^P1 has abnormal cell polarity phenotype, non-enhanceable by Krn^UAS.cUa/Scer\GAL4^sev.EP

(Brown and Freeman, 2003)

nmo^P1 has abnormal cell polarity phenotype, non-enhanceable by ru¹

(Brown and Freeman, 2003)

NOT suppressed by

Statement

Reference

nmo^P1/Df(3L)Exel6279 has abnormal locomotor rhythm phenotype, non-suppressible by Clk^out/Clk^Tag:V5.WT

(Mahesh et al., 2014)

nmo^P1 has abnormal cell polarity phenotype, non-suppressible by ru¹

(Brown and Freeman, 2003)

nmo^P1 has abnormal cell polarity phenotype, non-suppressible by Krn^UAS.cUa/Scer\GAL4^sev.EP

(Brown and Freeman, 2003)

nmo^j147-1/nmo^P1 has abnormal planar polarity phenotype, non-suppressible by hh^bar3/hh^bar3

(Chou and Chien, 2002)

Enhancer of

Statement

Reference

nmo^P1/nmo[+] is an enhancer of visible phenotype of Pkn^k06808

(Sass and Ostrow, 2014)

nmo^P1/nmo[+] is an enhancer of visible phenotype of Scer\GAL4^dpp.blk1, tkv^{Q253D.UAS.cNb}

(Zeng et al., 2007)

NOT Enhancer of

Statement

Reference

nmo^P1 is a non-enhancer of abnormal planar polarity | adult stage phenotype of Frl^DN.UAS, Scer\GAL4^sev.EP

(Dollar et al., 2016)

nmo^P1/nmo[+] is a non-enhancer of abnormal cell polarity phenotype of Scer\GAL4^hs.2sev, shg^{dCR3h.UAS.sgGFP}

(Mirkovic and Mlodzik, 2006)

Suppressor of

Statement

Reference

nmo^P1 is a suppressor of abnormal cell polarity phenotype of aos^rlt

(Gaengel and Mlodzik, 2003)

nmo^P1/nmo[+] is a suppressor of visible phenotype of rpr^GMR.PH

(Mirkovic et al., 2002)

NOT Suppressor of

Statement

Reference

nmo^P1/nmo[+] is a non-suppressor of abnormal cell polarity phenotype of Scer\GAL4^hs.2sev, shg^{dCR3h.UAS.sgGFP}

(Mirkovic and Mlodzik, 2006)

Other

Statement

Reference

Mad^k00237/Mad[+], nmo^P1 has abnormal neuroanatomy | dominant | third instar larval stage phenotype

(Merino et al., 2009)

Mad[+]/Mad⁶, nmo^P1 has abnormal neuroanatomy | dominant | third instar larval stage phenotype

(Merino et al., 2009)

dgo³⁰⁸, nmo^P1 has partially lethal - majority die phenotype

(Wu et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

nmo^P1 has ommatidium phenotype, enhanceable by Vang^stbm-6

(Mirkovic et al., 2011)

nmo^P1 has ommatidium phenotype, enhanceable by pk^sple-1

(Mirkovic et al., 2011)

NOT Enhanced by

Statement

Reference

nmo^P1 has ommatidium phenotype, non-enhanceable by Krn^UAS.cUa/Scer\GAL4^sev.EP

(Brown and Freeman, 2003)

nmo^P1 has ommatidium phenotype, non-enhanceable by ru¹

(Brown and Freeman, 2003)

nmo^P1 has eye phenotype, non-enhanceable by a⁷/Df(2R)a^EX1

(Liu and Lengyel, 2000)

NOT suppressed by

Statement

Reference

nmo^P1 has ommatidium phenotype, non-suppressible by Krn^UAS.cUa/Scer\GAL4^sev.EP

(Brown and Freeman, 2003)

nmo^P1 has ommatidium phenotype, non-suppressible by ru¹

(Brown and Freeman, 2003)

nmo^j147-1/nmo^P1 has ommatidium phenotype, non-suppressible by hh^bar3/hh^bar3

(Chou and Chien, 2002)

nmo^P1 has eye phenotype, non-suppressible by a⁷/Df(2R)a^EX1

(Liu and Lengyel, 2000)

Enhancer of

Statement

Reference

nmo^P1/nmo[+] is an enhancer of anterior wing margin phenotype of Pkn^k06808

(Sass and Ostrow, 2014)

nmo^P1/nmo[+] is an enhancer of wing vein | ectopic phenotype of Pkn^k06808

(Sass and Ostrow, 2014)

nmo^P1 is an enhancer of eye phenotype of Scer\GAL4^hs.2sev, miple1^UAS.Tag:HA

(Muñoz-Soriano et al., 2013)

nmo^P1 is an enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, miple1^UAS.Tag:HA

(Muñoz-Soriano et al., 2013)

nmo^P1 is an enhancer of eye phenotype of Scer\GAL4^GMR.PU, miple1^UAS.Tag:HA

(Muñoz-Soriano et al., 2013)

nmo^P1 is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PU, miple1^UAS.Tag:HA

(Muñoz-Soriano et al., 2013)

nmo^P1 is an enhancer of wing margin bristle | ectopic phenotype of dgo³⁰⁸

(Wu et al., 2008)

nmo^P1/nmo[+] is an enhancer of wing blade phenotype of Scer\GAL4^dpp.blk1, tkv^{Q253D.UAS.cNb}

(Zeng et al., 2007)

NOT Enhancer of

Statement

Reference

nmo^P1 is a non-enhancer of ommatidium phenotype of Frl^DN.UAS, Scer\GAL4^sev.EP

(Dollar et al., 2016)

nmo^P1/nmo[+] is a non-enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, shg^{dCR3h.UAS.sgGFP}

(Mirkovic and Mlodzik, 2006)

nmo^P1/nmo^P1 is a non-enhancer of eye phenotype of a⁷/Df(2R)a^EX1

(Liu and Lengyel, 2000)

Suppressor of

Statement

Reference

nmo^P1 is a suppressor of ommatidium phenotype of aos^rlt

(Gaengel and Mlodzik, 2003)

nmo^P1/nmo[+] is a suppressor of eye phenotype of rpr^GMR.PH

(Mirkovic et al., 2002)

NOT Suppressor of

Statement

Reference

nmo^P1/nmo[+] is a non-suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, shg^{dCR3h.UAS.sgGFP}

(Mirkovic and Mlodzik, 2006)

nmo^P1/nmo^P1 is a non-suppressor of eye phenotype of a⁷/Df(2R)a^EX1

(Liu and Lengyel, 2000)

Other

Statement

Reference

Mad^k00237/Mad[+], nmo^P1 has NMJ bouton | third instar larval stage phenotype

(Merino et al., 2009)

Mad[+]/Mad⁶, nmo^P1 has NMJ bouton | third instar larval stage phenotype

(Merino et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Presence of nmo^P1 does not significantly alter eye phenotypes seen in flies with Frl^DN.Scer\UAS driven by Scer\GAL4^sev.EP.

(Dollar et al., 2016)

Expression of Clk^T:SV5\V5.WT (in a Clk^out mutant background) does not alter the locomotor rhythm defects seen in nmo^P1/Df(3L)Exel6279.

Expression of Clk^{S859A.T:SV5\V5} (in a Clk^out mutant background) enhances the locomotor activity rhythm defects seen in nmo^P1/Df(3L)Exel6279 mutants.

(Mahesh et al., 2014)

Anterior wing margin and wing crossvein defects associated with Pkn^k06808 are enhanced by nmo^P1/+.

(Sass and Ostrow, 2014)

A nmo^P1 mutant background enhances the ommatidial rotation phenotype seen in flies expressing miple1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hs.2sev.

Dosage reduction of nmo, through a nmo^P1 mutant background is able to enhance the eye roughness seen in Scer\GAL4^GMR.PU>miple1^{Scer\UAS.T:Ivir\HA1} mutants.

(Muñoz-Soriano et al., 2013)

Many ommatidia do not rotate at all and remain oriented parallel to the equator in Vang^stbm-6 nmo^P1 and in pk^sple-1 nmo^P1 double mutants.

(Mirkovic et al., 2011)

Mad^k00237/+ ; nmo^P1/+ and Mad⁶/+ ; nmo^P1/+ double heterozygous third instar larvae show a significant reduction in the number of boutons per muscle surface area at the neuromuscular junction, in contrast to any of the single heterozygotes.

(Merino et al., 2009)

nmo^P1 enhances the ectopic wing margin bristle phenotype seen in dgo³⁰⁸ mutants. The majority of double mutant display extra margin bristles. Although some dgo³⁰⁸ nmo^P1 flies survive to adulthood, many die at larval or pupal stages.

(Wu et al., 2008)

The penetrance of the bifurcated wing phenotype caused by expression of tkv^{Q253D.Scer\UAS.cNb} under the control of Scer\GAL4^dpp.blk1 is enhanced by nmo^P1/+ from 20.8% to 86.3%.

(Zeng et al., 2007)

A nmo^P1/+ background does not affect the Scer\GAL4^hs.2sev>shg^{dCR3h.Scer\UAS.T:Avic\GFP-rs} ommatidial phenotype.

(Mirkovic and Mlodzik, 2006)

Krn^Scer\UAS.cUa, (driven by Scer\GAL4^sev.EP) or ru¹ has no effect on the nmo^P1 ommatidial phenotype.

(Brown and Freeman, 2003)

S^48-5/nmo^P1 mutants show 17.38%+-5.39 misrotated ommatidia compared to 9.55%+-1.43 seen in S^48-5 mutants alone.

(Gaengel and Mlodzik, 2003)

The arrested rotation phenotype of ommatidia in nmo^j147-1/nmo^P1 discs, is unaffected by hh^bar3/hh^bar3.

(Chou and Chien, 2002)

Heterozygosity for nmo^P1 partially suppresses the small eye size of rpr^GMR.PH animals.

(Mirkovic et al., 2002)

Has no effect on the eye phenotype produced by activated arm constructs. (either arm^S44Y.GMR or arm^S56F.GMR).

(Freeman and Bienz, 2001)

The addition of nmo^P1/nmo^P1 has no effect on a⁷/Df(2R)a^EX1 flies.

(Liu and Lengyel, 2000)

nmo^P1, fz³⁷ double mutant eyes display a large number of ommatidia that do not rotate at all compared to either nmo^P1 or fz³⁷ mutants alone.

(Zheng et al., 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

nmo^P1/nmo^DB is rescued by nmo^II.tub.GFP

(Mirkovic et al., 2011)

nmo^P1/Df(3L)Exel6279 is rescued by Scer\GAL4^tim.PU/nmo^UAS.cUa.GFP

(Yu et al., 2011)

nmo^P1/Df(3L)Exel6279 is rescued by Scer\GAL4^unspecified/nmo^UAS.cUa.GFP

(Merino et al., 2009)

Partially rescued by

nmo^P1 is partially rescued by nmo^UAS.cUa/Scer\GAL4^ct.PB

(Muñoz-Soriano et al., 2013)

nmo^P1/nmo^DB is partially rescued by nmo^UAS.cUa/Scer\GAL4^hs.2sev

(Mirkovic et al., 2011)

nmo^P1 is partially rescued by nmo^I.UAS.EGFP/Scer\GAL4^hs.2sev

(Fiehler and Wolff, 2008)

nmo^P1 is partially rescued by nmo^II.UAS.EGFP/Scer\GAL4^hs.2sev

(Fiehler and Wolff, 2008)

Not rescued by

nmo^P1/Df(3L)Exel6279 is not rescued by Scer\GAL4^G14/nmo^UAS.cUa.GFP

(Merino et al., 2009)

Comments

Expression of nmo^Scer\UAS.cUa under the control of Scer\GAL4^ct.PB in a nmo^P1 homozygous background rescues the reduction in ommatidial rotation angle, with the percentage of ommatidia rotating over 50[o] significantly higher than in nmo^P1 mutants.

(Muñoz-Soriano et al., 2013)

The reduced number of boutons per muscle surface area seen at the neuromuscular junction in nmo^P1/Df(3L)Exel6279 is rescued by neuronal expression of nmo^{Scer\UAS.cUa.T:Avic\GFP} under the control of Scer\GAL4^unspecified but not by expression of nmo^{Scer\UAS.cUa.T:Avic\GFP} under the control of Scer\GAL4^G14.

(Merino et al., 2009)

Expression of nmo^{II.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^hs.2sev partially rescues the nmo^P1 ommatidial rotation defect (in all 3 insertion lines tested).

Expression of nmo^{I.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^hs.2sev results in inconsistent rescue of the nmo^P1 ommatidial rotation defect (partial rescue is seen in 2 insertion lines, while one line almost completely rescues the defect).

(Fiehler and Wolff, 2008)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

27897

y¹ w^*; P{lacW}nmo^P1/TM3, Sb¹

Notes on Origin

Discoverer

Comments

Δ2-3 induced reversion of the mutant phenotype is accompanied by loss of the P-element from nmo^P1. nmo mutants are epistatic to argos^rlt with respect to ommatidial precluster orientation.

(Choi and Benzer, 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

(Weber et al., 2012)

nemo^P1

(Gaengel and Mlodzik, 2003, Freeman and Bienz, 2001)

nmo-P1

(Gaengel and Mlodzik, 2003)

nmo^P1

(Krzystek et al., 2023, White et al., 2020, Dollar et al., 2016, Muñoz-Soriano et al., 2016, Mahesh et al., 2014, Muñoz-Soriano et al., 2013, Yu et al., 2011, Fiehler and Wolff, 2008)

nmo^P

(Thuveson et al., 2019, Collu et al., 2018, Sass and Ostrow, 2014, Mirkovic et al., 2011, Mirkovic et al., 2011, Braid et al., 2010, Merino et al., 2009, Wu et al., 2008, Zeng et al., 2007, Mirkovic and Mlodzik, 2006, Zeng and Verheyen, 2004, Mirkovic et al., 2002, Verheyen et al., 2001)

Name Synonyms

Secondary FlyBase IDs

References (31)

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