FB2024_03 , released June 25, 2024
Allele: Dmel\oskS244A.S248A
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General Information
Symbol
Dmel\oskS244A.S248A
Species
D. melanogaster
Name
FlyBase ID
FBal0298352
Feature type
allele
Associated gene
Dmel\osk
Associated Insertion(s)
Carried in Construct
P{osk.S244A.S248A}
Key Links
Transgenic product class
missense_variant
(Morais-de-Sá et al., 2013)
polypeptide_post_translational_processing_variant
(Morais-de-Sá et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Morais-de-Sá et al., 2013)
polypeptide_post_translational_processing_variant
(Morais-de-Sá et al., 2013)
Mutagen
phiC31 integrase
(Morais-de-Sá et al., 2013)
in vitro construct
(Morais-de-Sá et al., 2013)
Progenitor genotype
Carried in construct
P{osk.S244A.S248A}
Cytology
Description

Amino acid replacement: S244A.

(Morais-de-Sá et al., 2013)

Amino acid replacement: S248A.

(Morais-de-Sá et al., 2013)

An osk genomic fragment containing a mutated osk coding sequence where the two phosphorylation sites are mutated, under the control of the endogenous promoter.

(Morais-de-Sá et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
osk
Encoded product / tool
osk
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      One copy of non-phosphorylatable oskS244A.S248A rescues abdomen formation in oskA87/Df(3R)p-XT103 but also produces a significant number of embryos with head defects. Approximately 20% of the embryos laid by females with two copies of oskS244A.S248A in a oskA87/Df(3R)p-XT103 background develop head defects and 10% exhibit symmetric bicaudals.

      Expression of oskS244A.S248A in a wild-type background produces three times as many embryos with head defects as in controls, with 19% bicaudal embryos.

      (Morais-de-Sá et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The presence of one copy of oskS244A.S248A exacerbates abdominal and head defects and increases the number of bicaudal embryos derived from mago1/Df(3R)p-XT103 mothers.

      (Morais-de-Sá et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      oskS244A.S248A partially rescues oskA87/Df(3R)p-XT103

      (Morais-de-Sá et al., 2013)
      Comments

      One copy of non-phosphorylatable oskS244A.S248A rescues abdomen formation in oskA87/Df(3R)p-XT103 but also produces a significant number of embryos with head defects. Approximately 20% of the embryos laid by females with two copies of oskS244A.S248A in a oskA87/Df(3R)p-XT103 background develop head defects and 10% exhibit symmetric bicaudals.

      (Morais-de-Sá et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      oskS244A.S248A
      Name Synonyms
      Secondary FlyBase IDs
        References (1)