FlyBase Allele Report: Dmel\mir-9a[sponge.UAS.mCherry]

General Information

Symbol

Dmel\mir-9a^{sponge.UAS.mCherry}

Species

D. melanogaster

Name

FlyBase ID

FBal0299691

Feature type

allele

Associated gene

Dmel\mir-9a

Associated Insertion(s)

Carried in Construct

P{UAS-mCherry.mir9a.sponge}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Loya et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-mCherry.mir9a.sponge}

Cytology

Description

UAS regulatory sequences drive expression of a mir-9a "sponge" sequence, which consists of ten repetitive mir-9a complementary sequences with mismatches at positions 9-12 separated by a variable four-nucleotide linker. The sponge sequence is inserted into 3'UTR sequence downstream of the mCherry coding region, to enhance stability.

(Loya et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mir-9a

Tagged with

mCherry

red fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates tauopathy

modeled by Hsap\MAPT^UAS.wt

(Subramanian et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased body size, with Scer\GAL4^Tub.PU, Scer\GAL80^ts.Ilp2

(Suh et al., 2015)

increased body size, with Scer\GAL4^Ilp2.PR

(Suh et al., 2015)

increased cell number, with Scer\GAL4^Ilp2.PR

(Suh et al., 2015)

increased cell size, with Scer\GAL4^Ilp2.PR

(Suh et al., 2015)

visible, with Scer\GAL4^Ilp2.PR

(Suh et al., 2015)

Phenotype Manifest In

wing, with Scer\GAL4^Ilp2.PR

(Suh et al., 2015)

Detailed Description

Statement

Reference

Expression of mir-9a^{sponge.Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^Hk-UH3 (during myofibril assembly) has no detrimental effect on flight ability of the emerging adults which also display no morphological abnormalities in their dorsal longitudinal muscles.

(Katti et al., 2017)

Expression of mir-9a^{sponge.Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^Ilp2.PR results in increases in body length, wing length and pupal volume. Haemolymph glucose and trehalose levels are reduced. Expression of mir-9a^{sponge.Scer\UAS.T:Disc\RFP-mCherry} in insulin-producing cells under the control of Scer\GAL4^Ilp2.PR increases wing cell size and number.

Expression of mir-9a^{sponge.Scer\UAS.T:Disc\RFP-mCherry} outside of the insulin-producing cells (under the control of Scer\GAL4^tub.PU and Scer\GAL80^ts.Ilp2 at the permissive temperature) results in a reduction in body length compared with controls but Ilp2 expression is unaffected. A significant reduction in body length is also seen when mir-9a^{sponge.Scer\UAS.T:Disc\RFP-mCherry} is expressed everywhere (at the restrictive temperature) but Ilp2 expression is increased.

(Suh et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Ilp2.PR, mir-9a^{sponge.UAS.mCherry} has visible phenotype, suppressible by sNPF-R[+]/sNPF-R^MI00427

(Suh et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

One copy of sNPF-R^MI00427 significantly suppresses the increase in body length seen when mir-9a^{sponge.Scer\UAS.T:Disc\RFP-mCherry} is expressed under the control of Scer\GAL4^Ilp2.PR.

(Suh et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

mir-9a^{sponge.UAS.mCherry}/Scer\GAL4^Hk-UH3 rescues mir-9a^UAS.pLUC

(Katti et al., 2017)

Comments

The inability to fly along with severe structural defects in their indirect flight muscles (broken muscle fibers, disorganized sarcomere) characteristic for flies expressing mir-9a^{Scer\UAS.T:Ppyr\LUC} under the control of Scer\GAL4^Hk-UH3 is rescued by co-expression of mir-9a^{sponge.Scer\UAS.T:Disc\RFP-mCherry}.

(Katti et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Disc\RFP^{mCherry.Scer\UAS.mir-9aSP}

mir-9a^{sponge.Scer\UAS.T:Disc\RFP-mCherry}

mir-9a^{sponge.Scer\UAS.T:Disc\RFP}

mir-9a^{sponge.UAS.mCherry}

Name Synonyms

Secondary FlyBase IDs

FBal0266094

References (5)

Report Sections

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