FB2024_03 , released June 25, 2024
Allele: Dmel\TaoRNAi.UAS.cHa
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General Information
Symbol
Dmel\TaoRNAi.UAS.cHa
Species
D. melanogaster
Name
FlyBase ID
FBal0300861
Feature type
allele
Associated gene
Dmel\Tao
Associated Insertion(s)
Carried in Construct
P{UAS-Tao.RNAi.H}
Key Links
Transgenic product class
RNAi_reagent
(Huang et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Huang et al., 2014)
Mutagen
in vitro construct
(Huang et al., 2014)
Progenitor genotype
Carried in construct
P{UAS-Tao.RNAi.H}
Cytology
Description

UAS regulatory sequences drive expression of an artificial microRNA (shRNA) targeting Tao.

(Huang et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Tao
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Adult flies expressing TaodsRNA.shRNA.Scer\UAS in the intestinal stem cells (ISCs) and enteroblasts (EBs) of the adult midgut for 7 days under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B contain an increased number of esg-positive cells in the midgut. These cells are often clustered, with aberrant morphology. Their cell size is increased, with many similar in size to enterocytes (ECs). Mitotic activity (PH3 staining) is significantly increased compared to controls. There is an increased in the number of Dl-positive ISC-like cells, EBs and enteroendocrine cells (ees).

      Intestinal stem cell clones expressing TaodsRNA.shRNA.Scer\UAS under the control of Scer\GAL4Act.PU are significantly larger than controls.

      (Huang et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of ykiHMS00041 suppresses the increase in esg-positive cells and mitosis seen when TaodsRNA.shRNA.Scer\UAS is expressed in adult midguts under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B.

      ykiB5 suppresses the increase in intestinal stem cell clone size seen when TaodsRNA.shRNA.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

      (Huang et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      TaoRNAi.UAS.cHa
      TaodsRNA.shRNA.Scer\UAS
      TaodsRNA.shRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)