FlyBase Allele Report: Dmel\Tak1[Ct.UASp.Tag:HA]

General Information

Symbol

Dmel\Tak1^{Ct.UASp.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0301280

Feature type

allele

Associated gene

Dmel\Tak1

Associated Insertion(s)

Carried in Construct

P{UASp-Tak1.Ct-HA}

Key Links

Transgenic product class

inframe_deletion

(Stronach et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Stronach et al., 2014)

Mutagen

in vitro construct

(Stronach et al., 2014)

Progenitor genotype

Carried in construct

P{UASp-Tak1.Ct-HA}

Cytology

Description

UASp regulatory sequences drive expression of amino acids 272-678 of Tak1, with the 5'UTR and starting methionine codon upstream. The construct is tagged at the C-terminal end with two copies of Tag:HA.

(Stronach et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Tak1

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage, with Scer\GAL4^GMR.PU

(Das et al., 2023)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, Tak1^{Ct.UASp.Tag:HA} has lethal - all die during embryonic stage phenotype, suppressible by spoon^KG02745

(Das et al., 2023)

Enhancer of

Statement

Reference

Scer\GAL4^arm.PS/Tak1^{Ct.UASp.Tag:HA} is an enhancer of partially lethal - majority die phenotype of slpr^BS06

(Stronach et al., 2014)

Suppressor of

Statement

Reference

Scer\GAL4^GMR.PF/Tak1^{Ct.UASp.Tag:HA} is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, egr^GS9830

(Stronach et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^GMR.PF/Tak1^{Ct.UASp.Tag:HA} is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, egr^GS9830

(Stronach et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Tak1^{Ct.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS further enhances the lethality associated with slpr^BS06 mutant males.

Expression of Tak1^{Ct.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS does not rescue the dorsal closure phenotype seen in slpr⁹²¹ mutant males. Some embryos exhibit head defects.

Expression of Tak1^{Ct.Scer\UAS.P\T.T:Ivir\HA1} suppresses the reduction in eye size seen when egr^GS9830 is expressed under the control of Scer\GAL4^GMR.PF.

(Stronach et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Scer\GAL4^da.G32/Tak1^{Ct.UASp.Tag:HA} fails to rescue Tak1[+]/Tak1²

(Stronach et al., 2014)

Comments

Expression of Tak1^{Ct.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 does not rescue the increased susceptibility to E.coli challenge seen in Tak1²/Y males. Expression of Tak1^{Ct.Scer\UAS.P\T.T:Ivir\HA1} under the control of either Scer\GAL4^da.G32 or Scer\GAL4^r4 does not increase susceptibility of Tak1²/+ females to E.coli.