FlyBase Allele Report: Dmel\tut[4]

General Information

Symbol

Dmel\tut⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0302045

Feature type

allele

Associated gene

Dmel\tut

Associated Insertion(s)

TI{TI}tut⁴

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

phiC31 integrase

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Chen et al., 2014)

Mutagen

phiC31 integrase

(Chen et al., 2014)

Progenitor genotype

tut³

(Chen et al., 2014)

Associated Insertion(s)

TI{TI}tut⁴

Cytology

Description

The tut genomic region has been introduced back into the attP site present in tut³. As a result of this manipulation, a 91bp fragment (attR site and vector sequence) is present upstream of the tut start codon, and a 68bp fragment (loxP and vector sequence) is present downstream of the tut stop codon. The presence of these fragments affects tut function.

(Chen et al., 2014)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | recessive

(Chen et al., 2014)

neoplasia | adult stage | male

(Shan et al., 2017)

Phenotype Manifest In

spermatogonium

(Shan et al., 2017, Chen et al., 2014)

testis

(Shan et al., 2017, Chen et al., 2014)

Detailed Description

Statement

Reference

tut⁴ homozygotes display relatively mild tumorigenic phenotype (due to spermatogonial overgrowth) in adult testes with large tumors (more than 1/6 of overall testis length) being very rare.

(Shan et al., 2017)

Homozygous testes contain mostly spermatocytes, mixed with a few spermatogonial tumours.

(Chen et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

tut⁴ has neoplasia | adult stage | male phenotype, enhanceable by Pcf11^{UAS.C.Tag:HA,Tag:FLAG}/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has neoplasia | adult stage | male phenotype, enhanceable by Pcf11^UAS.B/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has neoplasia | adult stage | male phenotype, enhanceable by Pcf11^UAS.D/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has neoplasia | adult stage | male phenotype, enhanceable by Wdr33^{UAS.A.Tag:HA,Tag:FLAG}/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has neoplasia | adult stage | male phenotype, enhanceable by Scer\GAL4^VP16.bam/Sym^UAS.A

(Shan et al., 2017)

tut⁴ has neoplasia | adult stage | male phenotype, enhanceable by Scer\GAL4^VP16.bam/IntS11^{UAS.A.Tag:HA,Tag:FLAG}

(Shan et al., 2017)

tut⁴ has neoplasia | adult stage | male phenotype, enhanceable by Scer\GAL4^VP16.bam/Cpsf73^UAS.A

(Shan et al., 2017)

tut⁴ has neoplasia | adult stage | male phenotype, enhanceable by Scer\GAL4^VP16.bam/IntS9^{UAS.A.Tag:HA,Tag:FLAG}

(Shan et al., 2017)

tut⁴ has neoplasia | adult stage | male phenotype, enhanceable by Cpsf5^{UAS.B.Tag:HA,Tag:FLAG}/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has neoplasia | adult stage | male phenotype, enhanceable by CG4612^{UAS.A.Tag:HA,Tag:FLAG}/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has neoplasia | adult stage | male phenotype, enhanceable by cbc^{UAS.A.Tag:HA,Tag:FLAG}/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has neoplasia | adult stage | male phenotype, enhanceable by CstF50^{UAS.A.Tag:HA,Tag:FLAG}/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has neoplasia | adult stage | male phenotype, enhanceable by Scer\GAL4^VP16.bam/Cpsf100^UAS.A

(Shan et al., 2017)

tut⁴ has neoplasia | adult stage | male phenotype, enhanceable by CstF64^UAS.A/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has neoplasia | adult stage | male phenotype, enhanceable by Not1^MI07631/Not1[+]

(Shan et al., 2017)

tut⁴ has neoplasia | adult stage | male phenotype, enhanceable by pAbp[+]/pAbp^k10109

(Shan et al., 2017)

tut⁴ has neoplasia | adult stage | male phenotype, enhanceable by pAbp^EY11561/pAbp^EY11561

(Shan et al., 2017)

tut⁴ has increased cell number | recessive phenotype, enhanceable by bam[+]/bam^Δ86

(Chen et al., 2014)

tut⁴ has increased cell number | recessive phenotype, enhanceable by bgcn[+]/bgcn^QS2

(Chen et al., 2014)

tut⁴ has increased cell number | recessive phenotype, enhanceable by bgcn[+]/bgcn²⁰⁰⁹³

(Chen et al., 2014)

NOT Enhanced by

Statement

Reference

tut⁴ has neoplasia | adult stage | male phenotype, non-enhanceable by Scer\GAL4^VP16.bam/Pabp2^{UAS.A.Tag:HA,Tag:FLAG}

(Shan et al., 2017)

tut⁴ has neoplasia | adult stage | male phenotype, non-enhanceable by Scer\GAL4^VP16.bam/su(f)^{UAS.E.Tag:HA,Tag:FLAG}

(Shan et al., 2017)

tut⁴ has neoplasia | adult stage | male phenotype, non-enhanceable by hrg^{UAS.A.Tag:HA,Tag:FLAG}/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has neoplasia | adult stage | male phenotype, non-enhanceable by Not3^KG10496/Not3^KG10496

(Shan et al., 2017)

tut⁴ has neoplasia | adult stage | male phenotype, non-enhanceable by PAN2^f00130/PAN2[+]

(Shan et al., 2017)

Enhancer of

Statement

Reference

tut⁴ is an enhancer of increased cell number | dominant phenotype of bam^Δ86

(Chen et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

tut⁴ has testis phenotype, enhanceable by Pcf11^{UAS.C.Tag:HA,Tag:FLAG}/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, enhanceable by Pcf11^{UAS.C.Tag:HA,Tag:FLAG}/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has testis phenotype, enhanceable by Pcf11^UAS.B/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, enhanceable by Pcf11^UAS.B/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has testis phenotype, enhanceable by Pcf11^UAS.D/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, enhanceable by Pcf11^UAS.D/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has testis phenotype, enhanceable by Wdr33^{UAS.A.Tag:HA,Tag:FLAG}/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, enhanceable by Wdr33^{UAS.A.Tag:HA,Tag:FLAG}/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has testis phenotype, enhanceable by Scer\GAL4^VP16.bam/Sym^UAS.A

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, enhanceable by Scer\GAL4^VP16.bam/Sym^UAS.A

(Shan et al., 2017)

tut⁴ has testis phenotype, enhanceable by Scer\GAL4^VP16.bam/IntS11^{UAS.A.Tag:HA,Tag:FLAG}

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, enhanceable by Scer\GAL4^VP16.bam/IntS11^{UAS.A.Tag:HA,Tag:FLAG}

(Shan et al., 2017)

tut⁴ has testis phenotype, enhanceable by Scer\GAL4^VP16.bam/Cpsf73^UAS.A

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, enhanceable by Scer\GAL4^VP16.bam/Cpsf73^UAS.A

(Shan et al., 2017)

tut⁴ has testis phenotype, enhanceable by Scer\GAL4^VP16.bam/IntS9^{UAS.A.Tag:HA,Tag:FLAG}

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, enhanceable by Scer\GAL4^VP16.bam/IntS9^{UAS.A.Tag:HA,Tag:FLAG}

(Shan et al., 2017)

tut⁴ has testis phenotype, enhanceable by Cpsf5^{UAS.B.Tag:HA,Tag:FLAG}/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, enhanceable by Cpsf5^{UAS.B.Tag:HA,Tag:FLAG}/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has testis phenotype, enhanceable by CG4612^{UAS.A.Tag:HA,Tag:FLAG}/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, enhanceable by CG4612^{UAS.A.Tag:HA,Tag:FLAG}/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has testis phenotype, enhanceable by cbc^{UAS.A.Tag:HA,Tag:FLAG}/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, enhanceable by cbc^{UAS.A.Tag:HA,Tag:FLAG}/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has testis phenotype, enhanceable by CstF50^{UAS.A.Tag:HA,Tag:FLAG}/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, enhanceable by CstF50^{UAS.A.Tag:HA,Tag:FLAG}/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has testis phenotype, enhanceable by Scer\GAL4^VP16.bam/Cpsf100^UAS.A

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, enhanceable by Scer\GAL4^VP16.bam/Cpsf100^UAS.A

(Shan et al., 2017)

tut⁴ has testis phenotype, enhanceable by CstF64^UAS.A/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, enhanceable by CstF64^UAS.A/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has testis phenotype, enhanceable by Not1^MI07631/Not1[+]

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, enhanceable by Not1^MI07631/Not1[+]

(Shan et al., 2017)

tut⁴ has testis phenotype, enhanceable by pAbp[+]/pAbp^k10109

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, enhanceable by pAbp[+]/pAbp^k10109

(Shan et al., 2017)

tut⁴ has testis phenotype, enhanceable by pAbp^EY11561/pAbp^EY11561

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, enhanceable by pAbp^EY11561/pAbp^EY11561

(Shan et al., 2017)

tut⁴ has testis phenotype, enhanceable by bam[+]/bam^Δ86

(Chen et al., 2014)

tut⁴ has spermatogonium phenotype, enhanceable by bam[+]/bam^Δ86

(Chen et al., 2014)

tut⁴ has testis phenotype, enhanceable by bgcn[+]/bgcn^QS2

(Chen et al., 2014)

tut⁴ has spermatogonium phenotype, enhanceable by bgcn[+]/bgcn^QS2

(Chen et al., 2014)

tut⁴ has testis phenotype, enhanceable by bgcn[+]/bgcn²⁰⁰⁹³

(Chen et al., 2014)

tut⁴ has spermatogonium phenotype, enhanceable by bgcn[+]/bgcn²⁰⁰⁹³

(Chen et al., 2014)

NOT Enhanced by

Statement

Reference

tut⁴ has testis phenotype, non-enhanceable by Scer\GAL4^VP16.bam/Pabp2^{UAS.A.Tag:HA,Tag:FLAG}

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, non-enhanceable by Scer\GAL4^VP16.bam/Pabp2^{UAS.A.Tag:HA,Tag:FLAG}

(Shan et al., 2017)

tut⁴ has testis phenotype, non-enhanceable by Scer\GAL4^VP16.bam/su(f)^{UAS.E.Tag:HA,Tag:FLAG}

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, non-enhanceable by Scer\GAL4^VP16.bam/su(f)^{UAS.E.Tag:HA,Tag:FLAG}

(Shan et al., 2017)

tut⁴ has testis phenotype, non-enhanceable by hrg^{UAS.A.Tag:HA,Tag:FLAG}/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, non-enhanceable by hrg^{UAS.A.Tag:HA,Tag:FLAG}/Scer\GAL4^VP16.bam

(Shan et al., 2017)

tut⁴ has testis phenotype, non-enhanceable by Not3^KG10496/Not3^KG10496

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, non-enhanceable by Not3^KG10496/Not3^KG10496

(Shan et al., 2017)

tut⁴ has testis phenotype, non-enhanceable by PAN2^f00130/PAN2[+]

(Shan et al., 2017)

tut⁴ has spermatogonium phenotype, non-enhanceable by PAN2^f00130/PAN2[+]

(Shan et al., 2017)

Enhancer of

Statement

Reference

tut⁴ is an enhancer of testis phenotype of bam^Δ86

(Chen et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The severity of the tut⁴ mutant tumorigenic phenotype is also strongly enhanced by combination with a single copy of either Not1^MI07631 or pAbp^k10109 or by pAbp^EY11561 homozygosity, no enhancement but rather an amelioration of the tumor phenotype is observed upon combination with Not3^KG10496 in homozygous or PAN2^f00130 in heterozygous state. None of these genetic perturbations cause a significant tumor phenotype if combined with just one copy of tut⁴.

(Shan et al., 2017)

Introducing bam^Δ86/+ into the tut⁴ background blocks germline development at spermatogonial stage.

Introducing bgcn²⁰⁰⁹³/+ or bgcn^QS2/+ into the tut⁴ background results in the majority of germ cells continuing to divide but unable to differentiate beyond spermatogonial stage.

(Chen et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (9)

Bloomington

76365