FB2024_02 , released April 23, 2024
Allele: Dmel\RhoGAP68FUAS.mCherry
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General Information
Symbol
Dmel\RhoGAP68FUAS.mCherry
Species
D. melanogaster
Name
FlyBase ID
FBal0304144
Feature type
allele
Associated gene
Dmel\RhoGAP68F
Associated Insertion(s)
Carried in Construct
P{UAS-mCherry-RhoGAP68F}
Key Links
Transgenic product class
wild_type
(de Madrid et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(de Madrid et al., 2015)
Mutagen
in vitro construct - regulatory fusion
(de Madrid et al., 2015)
in vitro construct - coding region fusion
(de Madrid et al., 2015)
Progenitor genotype
Carried in construct
P{UAS-mCherry-RhoGAP68F}
Cytology
Description

UASt regulatory sequences drive expression of RhoGAP68F that is tagged with mCherry.

(de Madrid et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
RhoGAP68F
Tagged with
mCherry
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Strong expression of RhoGAP68FScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Dll-md23 results in pupal lethality and necrosis of the tarsus.

      Moderate expression of RhoGAP68FScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Dll-md23 results in tarsal morphology defects, with a low incidence of broken tarsi. At the prepupal stage, 12% of legs show tears in the epithelium, proximal to the nascent tarsal joints. The tears form along the lateral membrane domain, below the adherens junctions and septate junctions and above the basal surface of the epithelium.

      Moderate expression of RhoGAP68FScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4ptc-559.1 alters the morphology and distribution of Rab4-positive endosomes in the leg disc epithelium. They show decreased accumulation in the apical region, increased accumulation in the medial region, enlargement and increased clustering.

      (de Madrid et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Rab7Scer\UAS.T:Avic\GFP dramatically increases the incidence of tears in the leg epithelium in animals with moderate expression of RhoGAP68FScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Dll-md23 (penetrance increases from 12% to 74%).

      (de Madrid et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      RhoGAP68FScer\UAS.T:Disc\RFP-mCherry
      RhoGAP68FUAS.mCherry
      Name Synonyms
      Secondary FlyBase IDs
        References (1)