FB2024_03 , released June 25, 2024
Allele: Dmel\rheaΔhead.GFP
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General Information
Symbol
Dmel\rheaΔhead.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0304191
Feature type
allele
Associated gene
Dmel\rhea
Associated Insertion(s)
Carried in Construct
P{GFP-talin.Δhead}
Key Links
Transgenic product class
inframe_deletion
(Klapholz et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Klapholz et al., 2015)
Mutagen
in vitro construct
(Klapholz et al., 2015)
Progenitor genotype
Carried in construct
P{GFP-talin.Δhead}
Cytology
Description

Fragment encoding a deleted form of rhea which lacks amino acids 1-450 and is tagged at the N-terminal end with GFP. It comprises 16284bp 5' of the rhea ATG start codon, the GFP tag, a 4 Ser linker, and rhea gene sequences extending from amino acid residue 451 through to 925bp 3' of the stop codon.

(Klapholz et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
rhea
Encoded product / tool
rhea
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Df(3L)79a embryos carrying rheaΔhead.T:Avic\GFP show complete muscle detachment (quantified as greater than 40% shortening of the dorsal muscles). Germband retraction is not affected in the mutant embryos.

      (Klapholz et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The expression of rheaΔhead.T:Avic\GFP in Df(3L)79a homozygous follicle cell clones leads to a strong reduction in the number of ectopic VincCO.Scer\UAS.P\T.T:Equa\eqFP578-TagRFP-positive cytoplasmic protein aggregates observed upon Scer\GAL4Act5C.PU-driven expression in those clones.

      (Maartens et al., 2016)

      More than 25% of Df(3L)79a, rheaΔhead.T:Avic\GFP Df(1)Vinc2 double mutant embryos fail to undergo germband retraction, a stronger phenotype than seen in Df(3L)79a, rheaΔhead.T:Avic\GFP single mutant embryos.

      (Klapholz et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      rheaΔhead.GFP
      rheaΔhead.T:Avic\GFP
      talinΔHead
      (Maartens et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)