FB2024_03 , released June 25, 2024
Allele: Dmel\Nhe2UAS.E358I.Tag:HA
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General Information
Symbol
Dmel\Nhe2UAS.E358I.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0304402
Feature type
allele
Associated gene
Dmel\Nhe2
Associated Insertion(s)
Carried in Construct
P{UAS-Nhe2-HA.E358I}
Also Known As
UAS-DNhe2E358I
Key Links
Transgenic product class
missense_variant
(Grillo-Hill et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Grillo-Hill et al., 2015)
Mutagen
in vitro construct
(Grillo-Hill et al., 2015)
Progenitor genotype
Carried in construct
P{UAS-Nhe2-HA.E358I}
Cytology
Description

UAS regulatory sequences drive expression of a mutant Nhe2 cDNA (E358I) tagged at the C-terminal end with Tag:HA.

(Grillo-Hill et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Nhe2
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of Nhe2UAS.E358I.Tag:HA under the control of Scer\GAL4109-30 leads to a significant decrease in intracellular pH in pre-follicle and follicle cells within the germarium, as compared to controls.

      (Ulmschneider et al., 2016)

      Externally, the retinae of flies expressing Nhe2Scer\UAS.E358I.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU show subtle defects in patterning, including rare bristle placement defects. Internally, no ommatidial defects are evident.

      The intracellular pH (pHi) in pupal eyes expressing Nhe2Scer\UAS.cGa under the control of Scer\GAL4GMR.PU is similar to controls.

      (Grillo-Hill et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The co-expression of Nhe2UAS.E358I.Tag:HA does not suppress the basal stalk multilayering observed upon the expression of ptcJF03223 under the control of Scer\GAL4109-30.

      (Ulmschneider et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Nhe2Scer\UAS.E358I.T:Ivir\HA1
      Nhe2UAS.E358I.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (3)