The R21B06 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
Comment: when combined with P{R13F02-p65.AD} (combination referred to as 'MB364B')
Comment: when combined with P{R13F02-p65.AD} (combination referred to as 'MB364B')
Comment: when combined with P{R13F02-p65.AD} (combination referred to as 'MB364B')
Comment: when combined with P{R13F02-p65.AD} (combination referred to as 'MB364B')
Comment: when combined with P{R13F02-p65.AD} (combination referred to as 'MB364B')
Comment: when combined with P{R13F02-p65.AD} (combination referred to as 'MB364B')
Comment: when combined with P{6xCRE-p65.AD}
when combined with P{elav-GAL4.AD}
Blocking synaptic transmission (using shits1.IVS.p10.20xScer\UAS at 33[o]C) from neurons targeted by the split-GAL4 combination of Scer\GAL4DBD.R21B06 and Hsap\RELAAD.R13F02 significantly impairs visual (aversive color discrimination conditioning) memory performance, compared to controls.
Hsap\RELAAD.VT036268, Hsap\TARDBPG298S.UAS, Scer\GAL4DBD.R21B06 has decreased efficacy of memory | adult stage phenotype, suppressible by dlpUAS.cBa/Hsap\RELAAD.VT036268, Scer\GAL4DBD.R21B06
Scer\GAL4DBD.R21B06, dlpUAS.cBa/Hsap\RELAAD.VT036268 is a suppressor of decreased efficacy of memory | adult stage phenotype of Hsap\RELAAD.VT036268, Hsap\TARDBPG298S.UAS, Scer\GAL4DBD.R21B06
Split-GAL4 transgene, constructed as described in FBrf0212052.