FB2024_03 , released June 25, 2024
Allele: Dmel\oskM1R.INV121-182.Tag:HA
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General Information
Symbol
Dmel\oskM1R.INV121-182.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0304913
Feature type
allele
Associated gene
Dmel\osk
Associated Insertion(s)
Carried in Construct
P{osk.M1R.INV121-182.HA}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Kanke and Macdonald, 2015)
Progenitor genotype
Carried in construct
P{osk.M1R.INV121-182.HA}
Cytology
Description

Expresses an osk transgene from a genomic fragment that includes the 5' extended UTR. The start codon for translation of isoform long-osk, M1, is mutated (as in oskM1R). Because long-osk is not produced by the transgene, mutations that lie between the start codons for long-osk and short-osk will only alter the mRNA sequence, and not the protein coding sequence. The transgene includes a 3xHA epitope tag, inserted after amino acid residue T140. The short-osk start codon is M139. Carries inversion 121-182 in the extended 5' UTR of osk.

(Kanke and Macdonald, 2015)
Allele components
Component
Use(s)
Regulatory region(s)
osk
Encoded product / tool
osk
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Segmentation defects are observed in embryos from oskA87/Df(3R)osk mothers expressing oskM1R.INV121-182.T:Ivir\HA1.

      (Kanke and Macdonald, 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      oskM1R.INV121-182.Tag:HA fails to rescue oskA87/Df(3R)osk

      (Kanke and Macdonald, 2015)
      Comments

      Segmentation defects are observed in embryos from oskA87/Df(3R)osk mothers expressing oskM1R.INV121-182.T:Ivir\HA1.

      (Kanke and Macdonald, 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      oskM1R.INV121-182.T:Ivir\HA1
      oskM1R.INV121-182.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (1)