FB2024_03 , released June 25, 2024
Allele: Dmel\foxoUAS.cKc
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General Information
Symbol
Dmel\foxoUAS.cKc
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Koyama
FlyBase ID
FBal0305269
Feature type
allele
Associated gene
Dmel\foxo
Associated Insertion(s)
Carried in Construct
PBac{UAS-foxo.Kc}
Key Links
Transgenic product class
wild_type
(Koyama et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Koyama et al., 2014)
Mutagen
in vitro construct
(Koyama et al., 2014)
Progenitor genotype
Carried in construct
PBac{UAS-foxo.Kc}
Cytology
Description

UASt regulatory sequences drive expression of wild type foxo.

(Koyama et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
foxo
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of foxoScer\UAS.cKc under the control of Scer\GAL4phm.PO results in a 10 hour delay in the 'critical weight' ecdysone peak compared to controls in response to starvation. Body size is increased when critical weight takes place.

      Expression of foxoScer\UAS.cKc under the control of Scer\GAL4phm.PO in starved larvae results in pharate adults with a increased body size compared to controls.

      Expression of foxoScer\UAS.cKc under the control of Scer\GAL4C-765 and Scer\GAL4Bx-MS1096 in the wing, and Scer\GAL4ey.PU and Scer\GAL4GMR.PU in the eye results in reduced organ size compared to controls.

      When foxoScer\UAS.cKc is expressed under the control of Scer\GAL4phm.PO in foxoΔ94/Df(3R)Exel8159 background most larvae do not survive to the third instar larval stage.

      Expression of foxoScer\UAS.cKc in a foxoΔ94/Df(3R)Exel8159 mutant background in the oenocytes under the control of Scer\GAL4Desat1.PB, or in the corpora allata under the control of Scer\GAL4Aug21 does not affect the duration of the third instar larval stage (L3) or final body size in starved larvae. Expression in the ring gland, oenocytes and the corpora allata under the control of Scer\GAL4P0206 delays metamorphosis and increases body size at the 'critical weight' ecdysone peak compared to parental controls. Feeding ecdysone to Scer\GAL4P0206>foxoScer\UAS.cKc foxoΔ94/Df(3R)Exel8159 starved early third instar larvae eliminates this delay in metamorphosis.

      Prothoracic gland size is reduced in larvae expressing foxoScer\UAS.cKc under the control of Scer\GAL4P0206.

      (Koyama et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      Scer\GAL4phtm.PO, foxoUAS.cKc has abnormal developmental rate | nutrition conditional phenotype, enhanceable by Scer\GAL4phtm.PO

      (Koyama et al., 2014)

      Scer\GAL4phtm.PO, foxoUAS.cKc has increased body size | pharate adult stage | nutrition conditional phenotype, enhanceable by uspUAS.cUa/Scer\GAL4phtm.PO

      (Koyama et al., 2014)
      Enhancer of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of uspScer\UAS.cUa enhances the delay in 'critical weight' ecdysone peak in response to starvation seen when foxoScer\UAS.cKc is expressed under the control of Scer\GAL4phm.PO. The increase in larval body size at critical weight is also enhanced.

      Co-expression of uspScer\UAS.cUa and foxoScer\UAS.cKc under the control of Scer\GAL4phm.PO enhances the increase in pharate adult body size seen following larval starvation compared with either transgene alone.

      (Koyama et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      foxoScer\UAS.cKc
      foxoUAS.cKc
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Koyama
      Secondary FlyBase IDs
        References (1)