FlyBase Allele Report: Dmel\path[A.UAS.GFP]

General Information

Symbol

Dmel\path^A.UAS.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0305311

Feature type

allele

Associated gene

Dmel\path

Associated Insertion(s)

Carried in Construct

P{UAS-path.A.GFP}

Key Links

Transgenic product class

wild_type

(Lin et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Lin et al., 2015)

Mutagen

in vitro construct

(Lin et al., 2015)

Progenitor genotype

Carried in construct

P{UAS-path.A.GFP}

Cytology

Description

UAS regulates expression of the long 'A' (471 aa) isoform of path tagged with GFP.

(Lin et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

path

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4²²¹

(Lin et al., 2015)

Phenotype Manifest In

larval multidendritic class I neuron, with Scer\GAL4²²¹

(Lin et al., 2015)

Detailed Description

Statement

Reference

Overexpression of path^{A.Scer\UAS.T:Avic\GFP} in C1da neurons of otherwise wild-type larvae using Scer\GAL4²²¹ promotes a modest but significant increase in C1da dendrite growth. In contrast, path^{A.Scer\UAS.T:Avic\GFP} overexpression using Scer\GAL4^ppk.PG has no effect on the total dendrite length of C4da neurons.

(Lin et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Rheb^UAS.cPa, Scer\GAL4^ppk.PG, path^A.UAS.GFP has abnormal neuroanatomy phenotype

(Lin et al., 2015)

Phenotype Manifest In

Other

Statement

Reference

Rheb^UAS.cPa, Scer\GAL4^ppk.PG, path^A.UAS.GFP has larval multidendritic class IV neuron phenotype

(Lin et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^ppk.PG-mediated co-expression of path^{A.Scer\UAS.T:Avic\GFP} and Rheb^Scer\UAS.cPa promote dendritic growth, whereas expression of either alone has no such effect.

(Lin et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Act5C.PI/path^A.UAS.GFP rescues path^dg50

(Lin et al., 2015)

path^A.UAS.GFP/Scer\GAL4^ppk.PG rescues path^dg50

(Lin et al., 2015)

Scer\GAL4^elav-C155/path^A.UAS.GFP rescues path^dg50/Df(3L)BSC773

(Lin et al., 2015)

path^A.UAS.GFP/Scer\GAL4^ppk.PG rescues path^dg50/Df(3L)BSC773

(Lin et al., 2015)

Scer\GAL4^21-7/path^A.UAS.GFP rescues path^dg50/Df(3L)BSC773

(Lin et al., 2015)

Partially rescues

path^A.UAS.GFP/Scer\GAL4^hs.PB partially rescues path^dg50/Df(3L)BSC773

(Lin et al., 2015)

Fails to rescue

Scer\GAL4^Mhc.PK/path^A.UAS.GFP fails to rescue path^dg50/Df(3L)BSC773

(Lin et al., 2015)

path^A.UAS.GFP/Scer\GAL4^OK72 fails to rescue path^dg50/Df(3L)BSC773

(Lin et al., 2015)

path^A.UAS.GFP/Scer\GAL4^A58 fails to rescue path^dg50/Df(3L)BSC773

(Lin et al., 2015)

Comments

Scer\GAL4^Act5C.PI-mediated expression of path^{A.Scer\UAS.T:Avic\GFP} rescues the dendrite defects in path^dg50 mutants.

A heat-shock induced pulse of expression of path^{A.Scer\UAS.T:Avic\GFP} (using Scer\GAL4^hs.PB) at 24, 48, 72 or 96 hours AEL is sufficient to significantly rescue dendrite growth defects of path^dg50/Df(3L)BSC773 animals.

path^{A.Scer\UAS.T:Avic\GFP} expression in path^dg50 MARCM clones rescues their C4da dendrite growth defects.

(Lin et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

path^{A.Scer\UAS.T:Avic\GFP}

path^A.UAS.GFP

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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