FB2024_03 , released June 25, 2024
Allele: Dmel\TrpγG4
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General Information
Symbol
Dmel\TrpγG4
Species
D. melanogaster
Name
FlyBase ID
FBal0318080
Feature type
allele
Associated gene
Dmel\Trpγ
Associated Insertion(s)
TI{GAL4}TrpγG4
Carried in Construct
Also Known As
trpγ-GAL4
Key Links
Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Akitake et al., 2015)
Mutagen
ends-out gene targeting
(Akitake et al., 2015)
Progenitor genotype
Associated Insertion(s)
TI{GAL4}TrpγG4
Cytology
Description

547bp of the Trpγ locus extending 3' from the first start codon have been deleted (this removes the first two ATGs present in Trpγ) and have been replaced by sequence encoding a GAL4 driver followed by a w+mW.hs marker.

(Akitake et al., 2015)
Allele components
Component
Use(s)
Inserted element
TI{GAL4}
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  autism spectrum disorder
      CEA with Trpγ1
      (Palacios-Muñoz et al., 2022)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Mutant flies show a small but significant defect in negative geotaxis.

      Mutant flies require four times as long as wild-type flies to complete the righting reflect.

      Mutant flies show significantly reduced speed and precision of movements during walking compared to controls.

      Homozygous, TrpγG4/Df(2L)ED1109 and TrpγG4/Trpγ1 flies initiate crossing of gaps at a frequency similar to controls, but their ability to successfully traverse larger gaps is impaired compared to wild type. The defect in homozygous is due to an inability to perform all the normal fine postural adjustments while attempting to cross the gap.

      Homozygous and TrpγG4/Trpγ1 tibial extensor muscles show a significant reduction in the action potential frequency in response to tibia deflections compared to controls.

      (Akitake et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      TrpγG4 is rescued by TrpγUAS.cAa/Scer\GAL4Trpγ-G4

      (Akitake et al., 2015)

      Trpγ1/TrpγG4 is rescued by TrpγUAS.cAa/Scer\GAL4Trpγ-G4

      (Akitake et al., 2015)
      Comments

      Expression of TrpγScer\UAS.cAa under the control of Scer\GAL4Trpγ-G4 rescues the increase in time required to complete the righting reflex which is seen in TrpγG4 flies.

      Expression of TrpγScer\UAS.cAa under the control of Scer\GAL4Trpγ-G4 in TrpγG4/Trpγ1 flies rescues the action potential frequency of tibial extensor muscles in response to tibia deflections to wild type and also rescues their ability to successfully traverse large gaps of 3.5mm to wild-type levels.

      (Akitake et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      TrpγG4
      trpγG4
      (Dhakal et al., 2022, Palacios-Muñoz et al., 2022, Akitake et al., 2015, Wong et al., 2014)
      trpγGal4
      (Tian et al., 2022)
      Name Synonyms
      Secondary FlyBase IDs
        References (6)