R566W
Analogous mutation in human TCF4 implicated in Pitt-Hopkins syndrome; mutation carried on in vitro construct; site of nucleotide substitution in fly gene inferred by FlyBase curator based on reported amino acid change.
Expression of daR566W.Scer\UAS under the control of Scer\GAL4pnr.PU reduces the number and size of thoracic bristles and causes malformation of the whole adult thorax.
Expression of daR566W.Scer\UAS under the control of two copies of Scer\GAL4GMR12B08 results in a rough eye phenotype and loss of eye pigmentation that is stronger in all females compared with males.
Constitutive expression of daR566W.Scer\UAS under the control of Scer\GAL4da.G32 leads to embryo lethality. This expression limited to the adult stages (using Scer\GAL80ts.αTub84B) results in lethality in 3-4 days.
Scer\GAL4da.G32/daR566W.UAS fails to rescue da10
Expression of daR566W.Scer\UAS under the control of Scer\GAL4da.G32 is unable to rescue the neuronal phenotypes seen in homozygous da10 mutant embryos.