FB2024_03 , released June 25, 2024
Allele: Dmel\SH3PX110A
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General Information
Symbol
Dmel\SH3PX110A
Species
D. melanogaster
Name
FlyBase ID
FBal0318727
Feature type
allele
Associated gene
Dmel\SH3PX1
Associated Insertion(s)
TI{TI}SH3PX110A
Carried in Construct
Key Links
Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Ukken et al., 2016)
Mutagen
CRISPR/Cas9
(Ukken et al., 2016)
gene targeting by homologous recombination
(Ukken et al., 2016)
Progenitor genotype
Associated Insertion(s)
TI{TI}SH3PX110A
Cytology
Description

The entire coding region has been deleted and replaced with the following sequence 1: a single attP site, 2: a loxP cassette containing the Disc\RFPDsRed2.3xP3 marker.

(Ukken et al., 2016)
Allele components
Component
Use(s)
Also carries
attP
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      SH3PX110A/SH3PX110A or SH3PX110A/Df(3L)BSC392 are viable with impaired fertility. SH3PX110A/Df(3L)BSC392 third instar larvae do not have a significantly different number of total boutons, satellite boutons or active zones per bouton at the neuromuscular junction (NMJ; counted at NMJ 4 and 6/7), compared to wild type. Mutants also have similar active zone density and active zone length compared to wild type; synaptic vesicle numbers are also similar to wild type, but SH3PX110A/Df(3L)BSC392 mutants show a small but significant increase in vesicle diameter at synapses and active zone T-bars shape is frequently malformed.

      SH3PX110A/Df(3L)BSC392 or SH3PX110A/SH3PX1C1 third instar larvae do not have significantly different miniature excitatory junction potential (mEJP) amplitude, but do have significant reduction in evoked EJP amplitude and quantal content (number of vesicles released per action potential), at the NMJ compared to controls.

      (Ukken et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Statement
      Reference

      SH3PX1C1/SH3PX110A is a non-suppressor of abnormal neurophysiology | third instar larval stage phenotype of nwk2

      (Ukken et al., 2016)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      SH3PX110A/SH3PX1C1 nwk2/nwk2 double mutants do not have significantly different quantal content deficits or further reduced evoked EJP amplitude compared to nwk2/nwk2 mutants at the third instar larval neuromuscular junction (though there is a trend towards reduced quantal content and EJP amplitude).

      (Ukken et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      SH3PX1C1/SH3PX110A is rescued by Scer\GAL4elav-C155/SH3PX1UAS.Tag:FLAG

      (Ukken et al., 2016)
      Not rescued by

      SH3PX1C1/SH3PX110A is not rescued by Scer\GAL4how-24B/SH3PX1UAS.Tag:FLAG

      (Ukken et al., 2016)
      Comments

      Neuronal (Scer\GAL4elav-C155) but not muscle (Scer\GAL4how-24B) driven expression of SH3PX1Scer\UAS.T:Zzzz\FLAG rescues quantal content and EJP amplitude to wild type levels at the neuromuscular junction of SH3PX110A/SH3PX1C1 third instar larvae.

      (Ukken et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      SH3PX110A
      (Ukken et al., 2016)
      Sh3px110A
      (Tsapras et al., 2022)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)