FlyBase Allele Report: Dmel\SH3PX1[C1]

General Information

Symbol

Dmel\SH3PX1^C1

Species

D. melanogaster

Name

FlyBase ID

FBal0318728

Feature type

allele

Associated gene

Dmel\SH3PX1

Associated Insertion(s)

TI{TI}SH3PX1^C1

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Ukken et al., 2016)

Mutagen

CRISPR/Cas9

(Ukken et al., 2016)

gene targeting by homologous recombination

(Ukken et al., 2016)

Progenitor genotype

Associated Insertion(s)

TI{TI}SH3PX1^C1

Cytology

Description

The entire coding region has been deleted and replaced with the following sequence 1: a single attP site, 2: a loxP cassette containing the Disc\RFP^DsRed2.3xP3 marker.

(Ukken et al., 2016)

Allele components

Component

Use(s)

Also carries

attP

integrase target site

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage (with SH3PX1^10A)

(Ukken et al., 2016)

muscle cell of A1-7 ventral longitudinal muscle 3 | third instar larval stage

(Wasserman et al., 2019)

Detailed Description

Statement

Reference

SH3PX1^C1/SH3PX1^C1 or SH3PX1^C1/Df(3L)BSC392 are viable with impaired fertility. SH3PX1^C1/Df(3L)BSC392 third instar larvae do not have a significantly different number of total boutons, satellite boutons or active zones at the neuromuscular junction (NMJ; counted at NMJ 4 and 6/7), compared to wild type.

SH3PX1^10A/SH3PX1^C1 third instar larvae do not have significantly different miniature excitatory junction potential (mEJP) amplitude, but do have significant reduction in evoked EJP amplitude and quantal content (number of vesicles released per action potential), at the NMJ compared to controls.

(Ukken et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

SH3PX1^C1 has abnormal locomotor behavior | third instar larval stage phenotype, non-enhanceable by Scer\GAL4^how-24B/Nedd4^{UAS.Long.Tag:FLAG}

(Wasserman et al., 2019)

Suppressed by

Statement

Reference

SH3PX1^C1 has abnormal neurophysiology | third instar larval stage phenotype, suppressible by Scer\GAL4^how-24B/Nedd4^{UAS.Long.Tag:FLAG}

(Wasserman et al., 2019)

NOT suppressed by

Statement

Reference

SH3PX1^C1 has abnormal locomotor behavior | third instar larval stage phenotype, non-suppressible by Scer\GAL4^how-24B/Nedd4^{UAS.Long.Tag:FLAG}

(Wasserman et al., 2019)

NOT Enhancer of

Statement

Reference

SH3PX1^C1 is a non-enhancer of abnormal locomotor behavior | third instar larval stage phenotype of Nedd4^{UAS.Long.Tag:FLAG}, Scer\GAL4^how-24B

(Wasserman et al., 2019)

NOT Suppressor of

Statement

Reference

SH3PX1^C1 is a non-suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Nedd4^{UAS.Long.Tag:FLAG}, Scer\GAL4^how-24B

(Wasserman et al., 2019)

SH3PX1^C1/SH3PX1^10A is a non-suppressor of abnormal neurophysiology | third instar larval stage phenotype of nwk²

(Ukken et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

SH3PX1^C1 has muscle cell of A1-7 ventral longitudinal muscle 3 | third instar larval stage phenotype, suppressible by Scer\GAL4^how-24B/Nedd4^{UAS.Long.Tag:FLAG}

(Wasserman et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

SH3PX1^10A/SH3PX1^C1 nwk²/nwk² double mutants do not have significantly different quantal content deficits or further reduced evoked EJP amplitude compared to nwk²/nwk² mutants at the third instar larval neuromuscular junction (though there is a trend towards reduced quantal content and EJP amplitude).

(Ukken et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

SH3PX1^C1/SH3PX1^10A is rescued by Scer\GAL4^elav-C155/SH3PX1^UAS.Tag:FLAG

(Ukken et al., 2016)

Not rescued by

SH3PX1^C1/SH3PX1^10A is not rescued by Scer\GAL4^how-24B/SH3PX1^UAS.Tag:FLAG

(Ukken et al., 2016)

Comments

Neuronal (Scer\GAL4^elav-C155) but not muscle (Scer\GAL4^how-24B) driven expression of SH3PX1^{Scer\UAS.T:Zzzz\FLAG} rescues quantal content and EJP amplitude to wild type levels at the neuromuscular junction of SH3PX1^10A/SH3PX1^C1 third instar larvae.

(Ukken et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

81609

w¹¹¹⁸; P{UAS-Nedd4.Lo.FLAG}2/CyO; TI{TI}SH3PX1^C1/TM3, P{ActGFP}JMR2, Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

SH3PX1^C1

(Wasserman et al., 2019, Ukken et al., 2016)

Sh3px1^C1

(Tsapras et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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