FlyBase Allele Report: Dmel\Arl2[T30N.UAS]

General Information

Symbol

Dmel\Arl2^T30N.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0318766

Feature type

allele

Associated gene

Dmel\Arl2

Associated Insertion(s)

Carried in Construct

P{UAS-Arl2.T30N}

Key Links

Transgenic product class

dominant_negative_variant

(Chen et al., 2016)

missense_variant

(Chen et al., 2016)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Chen et al., 2016)

missense_variant

(Chen et al., 2016)

Mutagen

in vitro construct

(Chen et al., 2016)

Progenitor genotype

Carried in construct

P{UAS-Arl2.T30N}

Cytology

Description

UAS regulates expression of Arl2 full-length coding sequence containing a T30N mutation, thus generating a dominant-negative form that mimics the constitutive GDP-bound form of Arl2.

(Chen et al., 2016)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Arl2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage, with Arl2^Δ156, Scer\GAL4^insc.PU

(Chen et al., 2016)

abnormal mitotic cell cycle | third instar larval stage, with Arl2^Δ156, Scer\GAL4^insc.PU

(Chen et al., 2016)

abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4^insc.PU

(Chen et al., 2016)

abnormal neuroanatomy | third instar larval stage, with Arl2^Δ156, Scer\GAL4^insc.PU

(Chen et al., 2016)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^insc.PU

(Chen et al., 2016)

increased cell number | somatic clone | third instar larval stage, with Scer\GAL4^ase.neuro

(Chen et al., 2016)

increased cell number | somatic clone | third instar larval stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Chen et al., 2016)

increased cell number | third instar larval stage, with Arl2^Δ156, Scer\GAL4^insc.PU

(Chen et al., 2016)

increased cell number | third instar larval stage, with Scer\GAL4^insc.PU

(Chen et al., 2016)

Phenotype Manifest In

larval neuroblast | increased number | third instar larval stage, with Arl2^Δ156, Scer\GAL4^insc.PU

(Chen et al., 2016)

larval neuroblast | increased number | third instar larval stage, with Scer\GAL4^insc.PU

(Chen et al., 2016)

larval neuroblast | somatic clone | increased number | third instar larval stage, with Scer\GAL4^ase.neuro

(Chen et al., 2016)

larval neuroblast | somatic clone | increased number | third instar larval stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Chen et al., 2016)

microtubule | third instar larval stage, with Scer\GAL4^insc.PU

(Chen et al., 2016)

spindle | third instar larval stage, with Arl2^Δ156, Scer\GAL4^insc.PU

(Chen et al., 2016)

spindle | third instar larval stage, with Scer\GAL4^insc.PU

(Chen et al., 2016)

type II neuroblast | somatic clone | third instar larval stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Chen et al., 2016)

type I neuroblast | somatic clone | third instar larval stage, with Scer\GAL4^ase.neuro

(Chen et al., 2016)

Detailed Description

Statement

Reference

Expression of Arl2^{T30N.Scer\UAS} driven by Scer\GAL4^insc.PU results in a significant increase in neuroblast number in the third instar larval central brain; introduction of heterozygous Arl2^Δ156/+ further enhances neuroblast overgrowth at 72hr (and even further at 84hr) after larval hatching at 29[o]C. Arl2^Δ156/+ larvae that also have expression of Arl2^{T30N.Scer\UAS} driven by Scer\GAL4^insc.PU have a slightly extended larval stage and more than half of metaphase neuroblasts have shorter misoriented spindles (compared to wild type), with a small percentage dividing orthogonally. Larvae with Arl2^{T30N.Scer\UAS} overexpressed by Scer\GAL4^insc.PU also have spindle misorientation in around 25% of neuroblasts, and around 13% of neuroblasts divide symmetrically. Unlike wild type telophase neuroblasts (where daughter cell sizes are distinct, with a NB/GMC diameter ratio of around 2), larvae with Arl2^{T30N.Scer\UAS} overexpressed by Scer\GAL4^insc.PU have a reduced ratio (around 1.65); introduction of Arl2^Δ156/+ further reduces the ratio (around 1.5).

Arl2^{T30N.Scer\UAS} clones have extra neuroblasts in both type I (Scer\GAL4^ase.neuro) and II (Scer\GAL4^wor.PA and Scer\GAL80^ase.PN) neuroblast lineages, compared to a single neuroblast in control clones (third instar larval central brain).

After treatment on ice (to depolymerize microtubules and disrupt mitotic spindles), in larvae with Arl2^{T30N.Scer\UAS} driven by Scer\GAL4^insc.PU around 40% of metaphase neuroblasts reassembled less microtubule mass after 30 seconds recovery and only 46% form mitotic spindles (that are shorter and more narrow), compared to wild type.

(Chen et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Arl2^Δ156, Arl2^T30N.UAS, Scer\GAL4^insc.PU has increased cell number | third instar larval stage phenotype, suppressible by msps^FL.UAS, Scer\GAL4^insc.PU

(Chen et al., 2016)

Arl2^Δ156, Arl2^T30N.UAS, Scer\GAL4^insc.PU has abnormal mitotic cell cycle | third instar larval stage phenotype, suppressible | partially by msps^FL.UAS, Scer\GAL4^insc.PU

(Chen et al., 2016)

Enhancer of

Statement

Reference

Arl2^T30N.UAS, Scer\GAL4^insc.PU is an enhancer of abnormal mitotic cell cycle | third instar larval stage phenotype of Scer\GAL4^insc.PU, Tbcd^UAS.cCa

(Chen et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Arl2^Δ156, Arl2^T30N.UAS, Scer\GAL4^insc.PU has larval neuroblast | third instar larval stage phenotype, suppressible by msps^FL.UAS, Scer\GAL4^insc.PU

(Chen et al., 2016)

Arl2^Δ156, Arl2^T30N.UAS, Scer\GAL4^insc.PU has spindle | third instar larval stage phenotype, suppressible | partially by msps^FL.UAS, Scer\GAL4^insc.PU

(Chen et al., 2016)

Enhancer of

Statement

Reference

Arl2^T30N.UAS, Scer\GAL4^insc.PU is an enhancer of spindle | third instar larval stage phenotype of Scer\GAL4^insc.PU, Tbcd^UAS.cCa

(Chen et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Arl2^{T30N.Scer\UAS} increases severity of microtubule growth defects (shorter spindles, inability to form bipolar spindle) in the central brain of third instar larvae with expression of TBCD^Scer\UAS.cCa driven by Scer\GAL4^insc.PU.

Co-expression of msps^FL.Scer\UAS dramatically reduces neuroblast number, largely restores spindle orientation in metaphase neuroblasts and rescues microtubule abnormalities (robust microtubule asters are present and spindle length is increased) in interphase neuroblasts in Arl2^Δ156/+ larvae with Arl2^{T30N.Scer\UAS} overexpressed by Scer\GAL4^insc.PU.

(Chen et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Arl2^T30N.UAS is rescued by Scer\GAL4^insc.PU/Arl2^WT.UAS

(Chen et al., 2016)

Comments

Co-expression of Arl2^WT.Scer\UAS rescues the overgrowth of neuroblasts seen in the central brain of third instar larvae with expression of Arl2^{T30N.Scer\UAS} driven by Scer\GAL4^insc.PU.

(Chen et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Arl2^{T30N.Scer\UAS}

Arl2^T30N.UAS

Name Synonyms

Secondary FlyBase IDs

References (1)

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