eye, with Scer\GAL4GMR.PF
retina, with Scer\GAL4GMR.PF
Expression of Disc\RFPCAG250.DsRed2.Scer\UAS under the control of Scer\GAL4da.PU results in compromised climbing ability at day one that progressively deteriorates over 14 days.
70% of flies expressing Disc\RFPCAG250.DsRed2.Scer\UAS under the control of Scer\GAL4da.PU display an abnormal wing posture, holding one or both wings up.
Expression of Disc\RFPCAG250.DsRed2.Scer\UAS in the developing eye, under the control of Scer\GAL4GMR.PF gives a weak rough eye phenotype, with some abnormal eye pigmentation and disruption of retinal integrity.
Co-expression of Disc\RFPCAG250.DsRed2.Scer\UAS and Disc\RFP(CTG)250.Scer\UAS in the eye, under the control of Scer\GAL4GMR.PF results in late developmental stage lethality. Dissection of animals from the pupal case reveals severely disrupted eye morphology.
Co-expression of Disc\RFPCAG250.DsRed2.Scer\UAS and Disc\RFP(CTG)200.Scer\UAS in the eye, under the control of Scer\GAL4GMR.PF, results in dramatic toxicity: the eye becomes severely rough with abnormal pigmentation and a severe loss of retinal integrity. This effect is synergistic as expression of either two copies of Disc\RFPCAG250.DsRed2.Scer\UAS or Disc\RFP(CTG)200.Scer\UAS alone has limited or no effect.
Co-expression of Disc\RFP(CTG)270.Scer\UAS with Disc\RFPCAG250.DsRed2.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF leads to a disrupted eye externally, with severe loss of retinal integrity internally.
Adult flies with a 30 minute heat shock induction of Disc\RFPCAG250.DsRed2.Scer\UAS (under the control of Scer\GAL4hs.PB) are viable and show no ill effects.
Adult flies with a 30 minute heat shock induction of Disc\RFPCAG250.DsRed2.Scer\UAS and Disc\RFP(CTG)250.Scer\UAS (both under the control of Scer\GAL4hs.PB) start to day within 24 hours, with approximately 90% of flies dead after 50 hours.
Flies expressing Disc\RFPCAG250.DsRed2.Scer\UAS in neurons under the control of Scer\GAL4elav-C155 die early with progressive loss of climbing ability compared to controls.
Scer\GAL4elav-C155, Zzzz\CAG250.UAS.DsRed2 has short lived phenotype, enhanceable by Hsap\MBNL1UAS.cGCa, Scer\GAL4elav-C155
Scer\GAL4GMR.PF, Zzzz\CAG250.UAS.DsRed2, Zzzz\CTG250.UAS.DsRed2 has increased mortality during development phenotype, suppressible by Dcr-2L811fsX
Scer\GAL4GMR.PF, Zzzz\CAG250.UAS.DsRed2, Zzzz\CTG200.UAS.DsRed2 has eye phenotype, suppressible by AGO2414
Scer\GAL4GMR.PF, Zzzz\CAG250.UAS.DsRed2, Zzzz\CTG200.UAS.DsRed2 has retina phenotype, suppressible by AGO2414
Expression of Hsap\HSPA1LScer\UAS.cWa suppresses the climbing defects seen when Disc\RFPCAG250.DsRed2.Scer\UAS is expressed under the control of Scer\GAL4da.PU.
Expression of Tpr2EB7-1A partially suppresses the wing posture and climbing defects seen when Disc\RFPCAG250.DsRed2.Scer\UAS is expressed under the control of Scer\GAL4da.PU.
Expression of wechE546 partially suppresses the wing posture and climbing defects seen when Disc\RFPCAG250.DsRed2.Scer\UAS is expressed under the control of Scer\GAL4da.PU.
Expression of orb2B8-S partially suppresses the wing posture and climbing defects seen when Disc\RFPCAG250.DsRed2.Scer\UAS is expressed under the control of Scer\GAL4da.PU.
A Dcr-2L811fsX homozygous mutant background suppresses the organismal lethality associated with 30 minute heat-shock induced expression of Disc\RFPCAG250.DsRed2.Scer\UAS and Disc\RFP(CTG)250.Scer\UAS (under the control of Scer\GAL4hs.PB).
A Dcr-2L811fsX homozygous mutant background has minimal effect on the toxicity associated with expression of Disc\RFPCAG250.DsRed2.Scer\UAS in the muscle (under the control of Scer\GAL4how-24B).
An AGO2414 homozygous background dramatically mitigates the toxicity found upon co-expression of Disc\RFP(CTG)200.Scer\UAS and Disc\RFPCAG250.DsRed2.Scer\UAS in the developing eye, under the control of Scer\GAL4GMR.PF.
Co-expression of Hsap\MBNL1Scer\UAS.cGCa further shortens the lifespan of flies expressing Disc\RFPCAG250.DsRed2.Scer\UAS pan-neuronally under the control of Scer\GAL4elav-C155.