FB2024_03 , released June 25, 2024
Allele: Dmel\glGFP.FPTB
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General Information
Symbol
Dmel\glGFP.FPTB
Species
D. melanogaster
Name
FlyBase ID
FBal0319845
Feature type
allele
Associated gene
Dmel\gl
Associated Insertion(s)
Carried in Construct
P{gl-GFP.FPTB}
PBac{gl-GFP.FPTB}
Key Links
Transgenic product class
genomic_DNA
(model organism Encyclopedia of Regulatory Network (modERN) Project, 2015-)
wild_type
(model organism Encyclopedia of Regulatory Network (modERN) Project, 2015-)
Mutagen
Nature of the Allele
Transgenic product class
genomic_DNA
(model organism Encyclopedia of Regulatory Network (modERN) Project, 2015-)
wild_type
(model organism Encyclopedia of Regulatory Network (modERN) Project, 2015-)
Mutagen
in vitro construct
(model organism Encyclopedia of Regulatory Network (modERN) Project, 2015-)
Progenitor genotype
Carried in construct
P{gl-GFP.FPTB}
PBac{gl-GFP.FPTB}
Cytology
Description

Genomic fragment including the gl gene, from BAC clone CH321-23N19. A sfGFP-Tag:FLAG-Tag:CS(PSP)-Tag:CS(TEVp)-Tag:BLRP tag cassette has been introduced at the C-terminal end of the gl gene, such that protein isoforms A and C are tagged.

(model organism Encyclopedia of Regulatory Network (modERN) Project, 2015-)
Allele components
Component
Use(s)
Regulatory region(s)
gl
Encoded product / tool
gl
Tagged with
sfGFP
Tag:BLRP
Tag:CS(PSP)
Tag:CS(TEVp)
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      glGFP.FPTB partially rescues gl3/gl60j

      (Liang et al., 2016)

      glGFP.FPTB partially rescues gl60j

      (Liang et al., 2016)
      Comments

      Expression of a single copy of glT:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP rescues the overall external morphology of the eye, but not the photoreceptor differentiation defects of adult gl3/gl60j and gl60j/gl60j mutants. In gl60j/gl60j mutants at 48 h APF, expression of a single copy of glT:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP significantly increases the number of ommatidia with the correct numbers of photoreceptor and cone cells.

      Expression of two copies of glT:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP rescues the external morphology of the eye and photoreceptor differentiation defects of gl60j/gl60j mutants.

      (Liang et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      glGFP.FPTB
      glT:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP
      glsfGFP,Tag:FLAG,Tag:CS(PSP),Tag:CS(TEVp),Tag:BLRP
      Name Synonyms
      Secondary FlyBase IDs
        References (2)