FB2024_03 , released June 25, 2024
Allele: Spyo\Cas9nanos.cPa
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General Information
Symbol
Spyo\Cas9nanos.cPa
Species
S. pyogenes
Name
FlyBase ID
FBal0321293
Feature type
allele
Associated gene
Spyo\Cas9
Associated Insertion(s)
Carried in Construct
M{nanos-Cas9.P}
P{nanos-Cas9.P}
PBac{nanos-Cas9.3xP3-EGFP}
Also Known As
nos-cas9
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Port and Bullock, 2014.1.10)
Progenitor genotype
Carried in construct
M{nanos-Cas9.P}
P{nanos-Cas9.P}
PBac{nanos-Cas9.3xP3-EGFP}
Cytology
Description

A human codon-optimized Cas9 endonuclease (cloned from Addgene plasmid 41815) is expressed under the control of nos promoter and 3' UTR sequences to give germline specific expression.

(Port and Bullock, 2014.1.10)
Allele components
Component
Use(s)
Regulatory region(s)
nanos
Encoded product / tool
Cas9
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Generated by Fillip Port and Simon Bullock, Medical Research Council, Cambridge.

      (Port and Bullock, 2014.1.10)

      Zzzz\Cas9nos.cPa allows efficient germ-line transmission of mutations without widespread targeting in somatic cells.

      (Port et al., 2014)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Spyo\Cas9nanos.cPa
      Spyo\Cas9nos.cPa
      Zzzz\Cas9nos.cPa
      Name Synonyms
      nanos construct a of Port
      Secondary FlyBase IDs
      • FBal0320679
      • FBal0294141
      References (26)