FB2024_03 , released June 25, 2024
Allele: Mmus\BaxUAS.cGa
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General Information
Symbol
Mmus\BaxUAS.cGa
Species
M. musculus
Name
Saccharomyces cerevisiae UAS construct a of Gaumer
FlyBase ID
FBal0322813
Feature type
allele
Associated gene
Mmus\Bax
Associated Insertion(s)
Carried in Construct
P{UAS-Bax.G}
Also Known As
UAS-bax
Transgenic product class
cDNA
(Gaumer et al., 2000)
wild_type
(Gaumer et al., 2000)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Gaumer et al., 2000)
wild_type
(Gaumer et al., 2000)
Mutagen
in vitro construct
(Blanchardon et al., 2001, Gaumer et al., 2000)
Progenitor genotype
Carried in construct
P{UAS-Bax.G}
Cytology
Description

UASt regulatory sequences drive expression of the full-length Mmus\Bax coding region (a EcoR1-XbaI fragment of Mmus\Bax cDNA).

(Gaumer et al., 2000)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Mmus\Bax
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Mmus\BaxScer\UAS.cGb under the control of Scer\GAL4Hml.Δ leads to increased susceptibility to Leishmania infection, with significantly increased lethality upon treatment with Leishmania amastigotes, but no significant difference in survival when untreated, as compared to wild type.

      (Okuda et al., 2016)

      Expression of Mmus\BaxScer\UAS.cGa under the control of Scer\GAL4vg.PM results in an increase in lethality, a notched wing phenotype of variable severity in adults and increased apoptosis in the wing disc in third instar larvae.

      Expression of Mmus\BaxScer\UAS.cGa under the control of Scer\GAL4da.G32 results in lethality at embryonic stages.

      (Colin et al., 2014)

      Expression of Mmus\BaxScer\UAS.cGa under the control of Scer\GAL4Gpb5 eliminates Gpb5/Gpa2 neuroendocrine cells.

      (Sellami et al., 2011)

      Mmus\BaxScer\UAS.cGa expression in the wing (under the control of Scer\GAL4vg.PM) leads to apoptosis in the wing tissue that is not essential to fly survival, thus adult wings exhibit notches on their wing margins. The phenotype is variable, with weak (notches scattered along the margin without significant change in wing size), intermediate (wings are reduced in size and wing margin is lost) and strong (partially or completely absent wings) phenotypes.

      (Colin et al., 2009)

      Scer\GAL4GMR.PF-mediated expression of Mmus\BaxScer\UAS.cGb results in eye ablation.

      (Galindo et al., 2009)

      Scer\GAL41118-driven Mmus\BaxScer\UAS.cGa expression results in 85% lethality, mostly at embryonic stages, and half of the surviving adults have shrivelled wings. Surviving individuals with expanded or shrivelled wings do not show major differences in their viability, at least up to 10-15 days of age.

      No clear rhythm is displayed in average activity plots by the Scer\GAL41118-driven Mmus\BaxScer\UAS.cGa expressing flies in constant darkness. At the individual level, 83% percent of the adult Scer\GAL41118-driven Mmus\BaxScer\UAS.cGa expressing flies display arrhythmic locomotor activity in constant darkness. The remaining rhythmic flies show a shortening of the period with a mean value of about 22.5h and a low power.

      In light dark conditions the Scer\GAL41118-driven Mmus\BaxScer\UAS.cGa expressing flies show a shift of evening activity peak of about 2h earlier than the controls.

      (Blanchardon et al., 2001)

      Ubiquitous expression of Mmus\BaxScer\UAS.cGb under the control of Scer\GAL4da.G32 affects the pattern of programmed cell death through embryonic development. For instance, from stage 11 to 13, the segmental pattern of dying cells along the antero-posterior axis normally found in wild-type embryos is disorganised. Cell aggregates, intensely-stained with Acridine Orange are seen in these embryos, likely as a result of engulfment of the apoptotic bodies by macrophages. The morphology of these embryos is hardly recognizable, and most of them die before hatching. Adults are rarely found.

      Mmus\BaxScer\UAS.cGb heterozygotes (i.e. with no Scer\GAL4 driver) are sub-viable. Flies homozygous for Mmus\BaxScer\UAS.cGb have a 2 day lifespan and reduced fertility. These flies also show melanotic masses at larval and adult stages.

      Eye-specific expression of Mmus\BaxScer\UAS.cGb (through Scer\GAL4GMR.PF) alters the ordered array of ommatidia and bristles, normally found in the wild-type in 45% of these flies. Many ommatidia are missing in these flies, with those remaining appearing smaller, with loss of pigments cells, photoreceptors and/or cone cells. Holes and vacuoles are also found, indicating massive levels of cell death.

      Expression of Mmus\BaxScer\UAS.cGb in the developing wing (through Scer\GAL4vg.PM) reduces the size of the wings in a temperature-dependent manner. At 18[o]C, most of the flies have notched wings, while at 21[o]C, the wing phenotype ranges from notched to absent wing. In some cases, the wing appears as a tubular structure, probably because the ventral and dorsal layers which make up the wing do not merge. At 25[o]C, most of the flies exhibit a gnarled or wrinkled wing phenotype.

      In the third instar larval imaginal wing disc, Mmus\BaxScer\UAS.cGb expression under the control of Scer\GAL4vg.PM at 18[o]C induces a modification in the size and shape of the disc. at 21[O]c, the wing part of the disc is reduced in size and highly disorganized.

      (Gaumer et al., 2000)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      NOT Enhanced by
      Statement
      Reference

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has visible | adult stage phenotype, non-enhanceable by morgueEP1184

      (Colin et al., 2014)
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has partially lethal phenotype, non-suppressible by Uba1[+]/Uba1s3484

      (Colin et al., 2014)

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has visible | adult stage phenotype, non-suppressible by Uba1[+]/Uba1s3484

      (Colin et al., 2014)

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has visible | adult stage phenotype, non-suppressible by morgueEP1184

      (Colin et al., 2014)

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has increased cell death phenotype, non-suppressible by Tom7[+]/Tom7BG02496

      (Colin et al., 2009)
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      Mmus\BaxUAS.cGa/Scer\GAL4He.PZ is a non-suppressor of melanotic mass phenotype | larval stage | nutrition conditional phenotype of MAPk-Ak2Δ43

      (Seisenbacher et al., 2011)
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has wing | adult stage phenotype, enhanceable by Prosα2EP931

      (Colin et al., 2014)

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has wing | adult stage phenotype, enhanceable by slmb8

      (Colin et al., 2014)

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has wing | adult stage phenotype, enhanceable by slmb41

      (Colin et al., 2014)

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has wing phenotype, enhanceable by unc-4503692/unc-45[+]

      (Colin et al., 2009)

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has wing margin phenotype, enhanceable by unc-4503692/unc-45[+]

      (Colin et al., 2009)
      NOT Enhanced by
      Statement
      Reference

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has wing | adult stage phenotype, non-enhanceable by morgueEP1184

      (Colin et al., 2014)
      Suppressed by
      Statement
      Reference

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has wing | adult stage phenotype, suppressible by Uba1UY3010, Scer\GAL4vg.PM

      (Colin et al., 2014)

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has wing | adult stage phenotype, suppressible by Uba1EP2375, Scer\GAL4vg.PM

      (Colin et al., 2014)

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has wing disc | third instar larval stage phenotype, suppressible by Uba1EP2375, Scer\GAL4vg.PM

      (Colin et al., 2014)

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has wing | adult stage phenotype, suppressible by Rpn10Pw+

      (Colin et al., 2014)

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has wing | adult stage phenotype, suppressible by SkpAEP1423, Scer\GAL4vg.PM

      (Colin et al., 2014)

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has wing phenotype, suppressible by mgeB10/mge[+]

      (Colin et al., 2009)

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has wing margin phenotype, suppressible by mgeB10/mge[+]

      (Colin et al., 2009)

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has wing phenotype, suppressible by Tom70KG06223/Tom70[+]

      (Colin et al., 2009)

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has wing margin phenotype, suppressible by Tom70KG06223/Tom70[+]

      (Colin et al., 2009)

      Mmus\BaxUAS.cGa, Scer\GAL4GMR.PF has eye phenotype, suppressible | partially by debcl[+]/DebclKO-unspecified

      (Galindo et al., 2009)

      Mmus\BaxUAS.cGa, Scer\GAL4GMR.PF has eye phenotype, suppressible by DebclKO-unspecified/DebclKO-unspecified

      (Galindo et al., 2009)

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has wing | temperature conditional phenotype, suppressible by Hsap\BCL2UAS.cGa, Scer\GAL4vg.PM

      (Gaumer et al., 2000)
      NOT suppressed by
      Statement
      Reference

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has wing | adult stage phenotype, non-suppressible by Uba1[+]/Uba1s3484

      (Colin et al., 2014)

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has wing | adult stage phenotype, non-suppressible by morgueEP1184

      (Colin et al., 2014)

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has wing phenotype, non-suppressible by Tom7[+]/Tom7BG02496

      (Colin et al., 2009)

      Mmus\BaxUAS.cGa, Scer\GAL4vg.PM has wing margin phenotype, non-suppressible by Tom7[+]/Tom7BG02496

      (Colin et al., 2009)
      NOT Suppressor of
      Statement
      Reference

      Mmus\BaxUAS.cGa/Scer\GAL4He.PZ is a non-suppressor of embryonic/larval hindgut | posterior | larval stage | nutrition conditional phenotype of MAPk-Ak2Δ43

      (Seisenbacher et al., 2011)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Over-expression of Uba1 with Uba1UY3010 under the control of Scer\GAL4vg.PM attenuates the notched wing phenotype and lethality observed in flies expressing Mmus\BaxScer\UAS.cGa under the control of Scer\GAL4vg.PM.

      Uba1s3484/+ fails to suppress the notched wing phenotype and lethality observed in flies expressing Mmus\BaxScer\UAS.cGa under the control of Scer\GAL4vg.PM.

      Co-expression of Uba1EP2375 almost completely suppresses the notched wing phenotype observed in adults, and reduces the number of apoptotic cells in third instar wing discs in flies expressing Mmus\BaxScer\UAS.cGa under the control of Scer\GAL4vg.PM.

      Co-expression of Uba1EP2375 partially rescues the lethality seen in flies expressing Mmus\BaxScer\UAS.cGa under the control of Scer\GAL4da.G32.

      Rpn10Pw+ partially suppresses the notched wing phenotype observed in flies expressing Mmus\BaxScer\UAS.cGa under the control of Scer\GAL4vg.PM.

      The presence of Prosα2EP931, slmb8 or slmb41 enhances the notched wing phenotype observed in flies expressing Mmus\BaxScer\UAS.cGa under the control of Scer\GAL4vg.PM.

      Co-expression of SkpAEP1423 partially suppresses the notched wing phenotype observed in flies expressing Mmus\BaxScer\UAS.cGa under the control of Scer\GAL4vg.PM.

      The presence of morgueEP1184 does not significantly affect the notched wing phenotype observed in flies expressing Mmus\BaxScer\UAS.cGa under the control of Scer\GAL4vg.PM.

      (Colin et al., 2014)

      Ablation of hemocytes (through expression of Mmus\BaxScer\UAS.cGb in the hemocytes under the control of Scer\GAL4He.PZ) does not suppress the "black dot" phenotype seen in MAPk-Ak2Δ43 mutant larvae.

      (Seisenbacher et al., 2011)

      A mgeB10 heterozygous background partially suppresses the wing phenotypes found upon expression of Mmus\BaxScer\UAS.cGa in the wing under the control of Scer\GAL4vg.PM. A shift in phenotype distribution from about 60% of strong and about 40% of intermediate phenotypes in the control cross to 30% of strong and about 70% of intermediate phenotypes in these double mutants.

      A Tom70KG06223 heterozygous background partially suppresses the wing phenotypes found upon expression of Mmus\BaxScer\UAS.cGa in the wing under the control of Scer\GAL4vg.PM.

      A Tom7BG02496 heterozygous background fails to suppresses the wing phenotypes found upon expression of Mmus\BaxScer\UAS.cGa in the wing under the control of Scer\GAL4vg.PM.

      A unc-4503692 heterozygous background enhances the wing phenotypes found upon expression of Mmus\BaxScer\UAS.cGa in the wing under the control of Scer\GAL4vg.PM. There is a significant increase from 60% to 90% of strong phenotypes (compared to controls) and a decrease from 40% to 10% of intermediate phenotypes. This shift in phenotype distribution reflects an enhancement of the Mmus\BaxScer\UAS.cGa-induced phenotype thus showing an increase of Mmus\BaxScer\UAS.cGa-induced cell death.

      (Colin et al., 2009)

      The eye ablation phenotype of Scer\GAL4GMR.PF, Mmus\BaxScer\UAS.cGb is partially restored in a debclKO-unspecified heterozygous background and nearly fully restored in a debclKO-unspecified homozygous background.

      (Galindo et al., 2009)

      Ubiquitous co-expression of Hsap\BCL2Scer\UAS.cGa and Mmus\BaxScer\UAS.cGb under the control of Scer\GAL4GMR.PF results in a pattern of cell death similar to flies expressing Hsap\BCL2Scer\UAS.cGa alone.

      Expression of Hsap\BCL2Scer\UAS.cGa is able to rescue the lethality of Mmus\BaxScer\UAS.cGb-expressing flies at 21[o]C (both under the control of Scer\GAL4vg.PM).

      Co-expression of Hsap\BCL2Scer\UAS.cGa in Mmus\BaxScer\UAS.cGb-expressing flies at 18[o]C induces a decrease in the ratio of strong wing phenotypes as opposed to intermediate and weak phenotypes and an increase of the ratio of weak wing phenotypes as opposed to intermediate and strong phenotypes. No significant effect was observed at 21 and 25[o]C. The structure of the wing is partially restored in these double mutants.

      (Gaumer et al., 2000)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Mmus\BaxScer\UAS.cGa
      Mmus\BaxScer\UAS.cGb
      Mmus\BaxUAS.cGa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Gaumer
      Secondary FlyBase IDs
      • FBal0240655
      • FBal0256959
      References (16)