Imprecise excision of P{EP}G9172 results in deletion of the Tnks transcription and translation initiation sites (-20 to +1839 with reference to the Tnks transcriptional start site); approximately 3.3K nucleotides from P{EP}G9172 remain at this site.
adult midgut (with Tnks19)
enteroblast | adult stage (with Tnks19)
Tnks19/Tnks503 transheterozygous mutants eclose at normal rates and are viable, but adults display a significant increase in mortality when maintained on a sucrose only diet (5% sucrose (v/v) in aqueous solution), as compared to controls.
Tnks19/Tnks503 transheterozygous mutant adult midguts show a significant increase in the numbers of intestinal stem cells, of intestinal progenitor cells and of mitotic cells, but similar apoptotic levels, as compared to controls; these phenotypes become more severe with age.
Tnks503/Tnks19 has increased cell number | adult stage phenotype, suppressible by Axn[+]/AxnS044230
Tnks503/Tnks19 has increased cell number | adult stage phenotype, suppressible by upd3KK110348/Scer\GAL4NP0001
Tnks503/Tnks19 has increased occurrence of cell division | adult stage phenotype, suppressible by upd3GD6811/Scer\GAL4NP0001
Tnks503/Tnks19 has increased occurrence of cell division | adult stage phenotype, suppressible by upd3KK110348/Scer\GAL4NP0001
Tnks503/Tnks19 has increased cell number | adult stage phenotype, suppressible by upd3GD6811/Scer\GAL4NP0001
Tnks503/Tnks19 has increased occurrence of cell division | adult stage phenotype, suppressible by Axn[+]/AxnS044230
AxnUAS.Tag:V5, Scer\GAL471B, Tnks503/Tnks19 has visible | adult stage phenotype
Tnks503/Tnks19 has adult midgut phenotype, suppressible by Axn[+]/AxnS044230
Tnks503/Tnks19 has enteroblast | adult stage phenotype, suppressible by Axn[+]/AxnS044230
Tnks503/Tnks19 has adult midgut phenotype, suppressible by upd3KK110348/Scer\GAL4NP0001
Tnks503/Tnks19 has intestinal stem cell | adult stage phenotype, suppressible by upd3KK110348/Scer\GAL4NP0001
Tnks503/Tnks19 has adult midgut phenotype, suppressible by upd3GD6811/Scer\GAL4NP0001
Tnks503/Tnks19 has intestinal stem cell | adult stage phenotype, suppressible by upd3GD6811/Scer\GAL4NP0001
AxnUAS.Tag:V5, Scer\GAL471B, Tnks503/Tnks19 has wing vein phenotype
AxnUAS.Tag:V5, Scer\GAL471B, Tnks503/Tnks19 has abdominal sternite bristle phenotype
AxnUAS.Tag:V5, Scer\GAL471B, Tnks503/Tnks19 has wing margin phenotype
AxnUAS.Tag:V5, Scer\GAL471B, Tnks503/Tnks19 has sternite phenotype
AxnUAS.Tag:V5, Scer\GAL471B, Tnks503/Tnks19 has wing margin bristle | ectopic phenotype
AxnUAS.Tag:V5, Scer\GAL471B, Tnks503/Tnks19 has wing blade phenotype
The increase in the numbers of intestinal progenitor cells and mitotic cells present in Tnks19/Tnks503 transheterozygous mutant adult midguts is suppressed by AxnS044230 heterozygosity; the increase in the numbers of intestinal stem cells and mitotic cells observed in Tnks19/Tnks503 transheterozygous mutant adult midguts are suppressed by expressing either upd3KK110348 or upd3GD6811 under the control of Scer\GAL4Myo31DF-NP0001.
Expression of AxnScer\UAS.T:SV5\V5 under the control of Scer\GAL471B in the Tnks19/Tnks503 background leads to morphological wing (notches, loss of wing margin, ectopic bristles) and ventral abdomen (loss of sternite and sternite bristles, reduced size of the sternites that remained, expansion of pleura) defects in adults.
Tnks503/Tnks19 is rescued by TnksUAS.Tag:HA/Scer\GAL4NP0001
Expressing TnksScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Myo31DF-NP0001 significantly rescues the increase in the numbers of stem cells and mitotic cells observed in the adult midgut of Tnks19/Tnks503 transheterozygous mutant adults.