FB2024_03 , released June 25, 2024
Allele: Dmel\Duba107
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General Information
Symbol
Dmel\Duba107
Species
D. melanogaster
Name
FlyBase ID
FBal0324253
Feature type
allele
Associated gene
Dmel\Duba
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Koerver et al., 2016)
Mutagen
P-element activity
(Koerver et al., 2016)
Progenitor genotype
P{EP}DubaG18421
(Koerver et al., 2016)
Cytology
Description

Deletion of the entire coding sequence. Generated by imprecise excision of P{EP}DubaG18421.

(Koerver et al., 2016)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Duba107 homozygotes are viable and develop normally, they do not show any defects in developmental cell death during either eye (removal of interommatidial cells) or arista (branching pattern) development but homozygote males (not females) are sterile and their seminal vesicles do not contain any mature sperm. Duba107 mutant males show defects in spermatid individualization (the spermatids appear thicker and disorganized and the individualization complex, cystic bulges as well as the waste bags are not detectable) and the activity of the caspases involved in the individualization process is reduced. Strong accumulation of ubiquitylated proteins is observed in the mutant testes.

      (Koerver et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      Duba107/Duba[+] is a suppressor | partially of visible | adult stage phenotype of rprGMR.PU

      (Koerver et al., 2016)

      Duba107/Duba107 is a suppressor of visible | adult stage phenotype of hidGMR.PG

      (Koerver et al., 2016)
      Phenotype Manifest In
      NOT suppressed by
      Suppressor of
      Statement
      Reference

      Duba107/Duba[+] is a suppressor | partially of eye | adult stage phenotype of rprGMR.PU

      (Koerver et al., 2016)

      Duba107/Duba107 is a suppressor of eye | adult stage phenotype of hidGMR.PG

      (Koerver et al., 2016)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The strongly reduced eye size characteristic for adult flies expressing either rprGMR.PU or hidGMR.PG is partially reverted by combination with Duba107.

      The spermatogenesis defects characteristic for Duba107 homozygote adult males are not suppressed by expression of any of the following: Droncpro.C-A.Scer\UAS, DriceScer\UAS.T:SV5\V5 or DriceC-A.Scer\UAS.T:SV5\V5 under the control of Scer\GAL4Hsp83.PA in the mutant background as mature spermatids are completely missing in these flies.

      (Koerver et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Duba107 is rescued by Scer\GAL4VP16.bam/DubaUAS.Tag:HA

      (Koerver et al., 2016)
      Not rescued by

      Duba107 is not rescued by DubaC227A.UAS.Tag:HA/Scer\GAL4VP16.bam

      (Koerver et al., 2016)

      Duba107 is not rescued by DubaS183A.UAS.Tag:HA/Scer\GAL4VP16.bam

      (Koerver et al., 2016)
      Comments

      The spermatogenesis defects and male sterility characteristic for Duba107 homozygote males is rescued by expression of DubaScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4bam.T:Hsim\VP16 in the mutant background, whereas expression of either DubaC227A.Scer\UAS.T:Ivir\HA1 or DubaS183A.Scer\UAS.T:Ivir\HA1 fails to rescue the mutant phenotype - on the contrary, the spermatid structures seem to be disrupted even more.

      (Koerver et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Duba107
      (Koerver et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)