FB2024_03 , released June 25, 2024
Allele: Dmel\Droncpro.C-A.UAS
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General Information
Symbol
Dmel\Droncpro.C-A.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0104746
Feature type
allele
Associated gene
Dmel\Dronc
Associated Insertion(s)
Carried in Construct
P{UAS-pro-dronc.C->A}
Key Links
Transgenic product class
missense_variant
(Meier, 2000.4.5)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Meier, 2000.4.5)
Mutagen
in vitro construct
(Meier, 2000.4.5)
Progenitor genotype
Carried in construct
P{UAS-pro-dronc.C->A}
Cytology
Description

A catalytically inactive coding sequence of Dronc is expressed under the control of UASt sequences. Includes nucleotides 369 to 1721, with its catalytic Cys mutated to Ala.

(Meier, 2000.4.5)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Dronc
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Droncpro.C-A.Scer\UAS under the control of Scer\GAL4Hsp83.PA has no significant effect on spermatogenesis in adult flies.

      (Koerver et al., 2016)

      Adult eyes of flies overexpressing dominant-negative Nc, under the control of Scer\GAL4GMR.PF, appear phenotypically normal, with precocious cell death not induced in these eye-antennal discs,

      (Verghese et al., 2012)

      22% of heat shocked Ncpro.C-A.Scer\UAS; Scer\GAL4hs.PB pupae have persistent salivary glands at 24 hours apf (n=383), whereas no salivary gland cells persist in non heat shocked control pupae with the same genotype. DNA fragmentation fails to occur in these persistent salivary glands. 10% of heat shocked Ncpro.C-A.Scer\UAS; Scer\GAL4fkh.PH pupae have persistent salivary glands at 24 hours apf (n=122). DNA fragmentation fails to occur in these persistent salivary glands.

      (Martin and Baehrecke, 2004)

      The addition of Ncpro.C-A.Scer\UAS driven by Scer\GAL4GMR.PF, to rprGMR.PW embryos partially suppresses cell killing in the eye.

      (Meier et al., 2000)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      Droncpro.C-A.UAS/Scer\GAL4GMR.PF is a suppressor of phenotype of rprGMR.PW

      (Meier et al., 2000)
      NOT Suppressor of
      Statement
      Reference

      Droncpro.C-A.UAS/Scer\GAL4Hsp83.PA is a non-suppressor of spermatid | adult stage phenotype of Duba107

      (Koerver et al., 2016)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The spermatogenesis defects characteristic for Duba107 homozygote adult males are not suppressed by expression of Droncpro.C-A.Scer\UAS under the control of Scer\GAL4Hsp83.PA in the mutant background as mature spermatids are completely missing in these flies.

      (Koerver et al., 2016)

      Co-expression of Ncpro.C-A.Scer\UAS with hpoScer\UAS.cUa in the developing eye (both under the control of Scer\GAL4GMR.PF completely suppresses the cell death seen upon expression of hpoScer\UAS.cUa alone.

      (Verghese et al., 2012)

      Co-expression of catalytically inactive Ncpro.C-A.Scer\UAS attenuates numbTS4D.Scer\UAS induced ectopic neuroblast formation.

      (Ouyang et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Droncpro.C-A.UAS rescues DroncI29/DroncL32

      (Napoletano et al., 2017)
      Comments

      The expression of Droncpro.C-A.Scer\UAS under the control of Scer\GAL4nos.PU rescues both the significant proportion of hyperplastic adult testes and the increased necrosis at the apical tip of testes observed in DroncI29/DroncL32 transheterozygotes.

      (Napoletano et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Droncpro.C-A.Scer\UAS
      Droncpro.C-A.UAS
      FBal0104746
      (Koerver et al., 2016)
      Ncpro.C-A.Scer\UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (8)