FB2024_03 , released June 25, 2024
Allele: Dmel\Mettl3Delta22-3
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General Information
Symbol
Dmel\Mettl3Delta22-3
Species
D. melanogaster
Name
FlyBase ID
FBal0324790
Feature type
allele
Associated gene
Dmel\Mettl3
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Haussmann et al., 2016)
Mutagen
P-element activity
(Haussmann et al., 2016)
Progenitor genotype
P{SUPor-P}KrT95DKG02054
(Haussmann et al., 2016)
Cytology
Description

Imprecise excision of P{SUPor-P}KrT95DKG02054, resulting in deletion of most of the protein-coding region of Ime4, including the catalytic domain.

(Haussmann et al., 2016)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3R:24,031,801..24,033,824 [+]
Comment:

A 2024 bp deletion resulting from the imprecise excision of P{SUPor-P}KrT95DKG02054 removes most of the Mettl3 coding sequence.

(Soller and Song, 2017.2.17)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Ime4Δ22-3 mutants are viable and fertile and homozygous females do not display any ovarian defects. Ime4Δ22-3/Df(3R)Exel6197 flies are flightless and the viability of females is reduced to 60% compared to the number of males.

      (Haussmann et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Ime4Δ22-3;SxlfP7B0 double heterozygous daughters from Ime4Δ22-3 mutant females show strongly reduced female viability compared to controls and tumorous ovaries (no decrease in viability or ovarian defects are observed in the presence of maternal wild-type copy of Ime4). The reduced viability phenotype can be fully rescued by combination with msl-2227/Df(2L)Exel7016 although these females frequently show signs of sexual transformation and display male-specific features such as sex-combs, which are mosaic to various degrees.

      The female lethality characteristic for either fl(2)d1 or vir2f homozygotes is rescued to a high degree by combination with a single copy of Ime4Δ22-3.

      The male lethality of flies expressing YT521-BScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4tub.PU is partially rescued by combination with Ime4Δ22-3 in a homozygous state (although the viability of such males is still much lower compared to controls).

      (Haussmann et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Mettl3Delta22-3/Ime4[+] is rescued by Mettl3Tag:HA

      (Haussmann et al., 2016)
      Partially rescued by

      Mettl3Delta22-3/Df(3R)Exel6197 is partially rescued by Mettl3Tag:HA

      (Haussmann et al., 2016)
      Comments

      The flightless phenotype characteristic for Ime4Δ22-3/Df(3R)Exel6197 mutant flies is rescued to a high degree by combination with Ime4T:Ivir\HA1.

      The severely decreased viability of SxlfP7B0;Ime4Δ22-3 double heterozygous daughters from Ime4Δ22-3 females is also restored by combination with Ime4T:Ivir\HA1.

      (Haussmann et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Ime4Delta22-3
      (Soller and Song, 2017.2.17)
      Ime4null
      (Haussmann et al., 2016)
      Ime4Δ22-3
      (Haussmann et al., 2016)
      Mettl3Delta22-3
      Name Synonyms
      Secondary FlyBase IDs
        References (2)