FB2024_03 , released June 25, 2024
Allele: Dmel\His1RNAi.UAS.cLa
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General Information
Symbol
Dmel\His1RNAi.UAS.cLa
Species
D. melanogaster
Name
FlyBase ID
FBal0325681
Feature type
allele
Associated gene
His1
Associated Insertion(s)
Carried in Construct
P{UAS-His1.RNAi.L}
Also Known As
pINT-1-H1
Key Links
Transgenic product class
RNAi_reagent
(Lu et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Lu et al., 2009)
Mutagen
in vitro construct
(Lu et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-His1.RNAi.L}
Cytology
Description

UAS regulatory sequences drive expression of an inverted repeat of the first 600bp of the His1 coding sequence. The two copies of the inverted repeat are separated by a spacer (the first intron of the Act5C gene).

(Lu et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
His1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of His1dsRNA.Scer\UAS.cLa under the control of Scer\GAL4tub.PU leads to partial (at lower temperature 18[o]C or 22[o]C) or complete (at higher temperature 26[o]C or 29[o]C) lethality. At 29[o] the animals survive through the third larval instar but the larvae have smaller wing imaginal discs as well as salivary glands that contain fewer cells with enlarged nuclei, furthermore the morphology of the polytene chromosomes is abnormal as the sister chromatids are mis-aligned and the appearance of the chromocenter is disrupted.

      The position effect variegation at the Sb or y locus caused by T(2;3)SbV or P{SUPor-P}KV00111, respectively is suppressed in flies expressing His1dsRNA.Scer\UAS.cLa driven by Scer\GAL4tub.PU.

      (Lu et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      His1RNAi.UAS.cLa, Scer\GAL4Tub.PU has lethal phenotype, suppressible by Dp(2;2)Cam8

      (Lu et al., 2009)

      His1RNAi.UAS.cLa, Scer\GAL4Tub.PU has lethal phenotype, suppressible by Dp(2;Y)H1/Dp(2;1)C239

      (Lu et al., 2009)

      His1RNAi.UAS.cLa, Scer\GAL4Tub.PU has lethal phenotype, suppressible by Dp(2;f)Bl

      (Lu et al., 2009)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The complete lethality of flies expressing His1dsRNA.Scer\UAS.cLa under the control of Scer\GAL4tub.PU when raised at 29[o]C is alleviated by combination with Dp(2;2)Cam8 or Dp(2;f)Bl or Dp(2;1)C239 combined with Dp(2;Y)H1.

      (Lu et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      His1RNAi.UAS.cLa
      His1dsRNA.Scer\UAS.cLa
      His1dsRNA.UAS.cLa
      Name Synonyms
      Secondary FlyBase IDs
        References (5)