FlyBase Allele Report: Avic\GFP[EGFP.UAS.Tag:PH(hPLCD1)]

General Information

Symbol

Avic\GFP^{EGFP.UAS.Tag:PH(hPLCD1)}

Species

A. victoria

Name

FlyBase ID

FBal0340412

Feature type

allele

Associated gene

Avic\GFP

Associated Insertion(s)

Carried in Construct

P{UAS-PLCδ-PH-EGFP}

Also Known As

UAS-PLCδ-PH-EGFP, UAS-PLCΔPH-GFP, UAS-PLCδ-PH-GFP, UAS-PLCδ1-PH-GFP

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Verstreken et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-PLCδ-PH-EGFP}

Cytology

Description

UAS regulatory sequences drive expression of EGFP tagged with Tag:PH(hPLCD1). Used to monitor the distribution of phosphatidylinositol 4,5-bisphosphate (PIP2) within the cell.

(Verstreken et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

EGFP

green fluorescent protein

Tagged with

Tag:PH(hPLCD1)

small molecule sensor

protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^elav-C155

(Khuong et al., 2010)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^nSyb.PS

(Khuong et al., 2010)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^Toll-6-D42

(Khuong et al., 2010)

synaptic vesicle, with Scer\GAL4^nSyb.PS

(Khuong et al., 2010)

Detailed Description

Statement

Reference

Expression of Hsap\PLCD1^{PH.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^n-syb.PS results in neuromuscular junction (NMJ) overgrowth in third instar larvae, including increased total branch length and satellite boutons. A less severe increase in NMJ length is seen when Hsap\PLCD1^{PH.Scer\UAS.T:Avic\GFP-EGFP} is expressed under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^D42.

Expression of Hsap\PLCD1^{PH.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^n-syb.PS results in reduced synaptic vesicle cycling (reduced uptake of FM 1-43 dye) during intense stimulation. Neurotransmitter characteristics (excitatory junctional current (EJC) and miniature EJC) are similar to controls.

(Khuong et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^pnr-MD237/Avic\GFP^{EGFP.UAS.Tag:PH(hPLCD1)} is a suppressor | partially of abnormal neuroanatomy | adult stage phenotype of PRL-1⁶/PRL-1²

(Urwyler et al., 2019)

Scer\GAL4^pnr-MD237/Avic\GFP^{EGFP.UAS.Tag:PH(hPLCD1)} is a suppressor | partially of abnormal size | adult stage phenotype of PRL-1⁶/PRL-1²

(Urwyler et al., 2019)

Scer\GAL4^nSyb.PS/Avic\GFP^{EGFP.UAS.Tag:PH(hPLCD1)} is a suppressor | partially of abnormal neuroanatomy phenotype of mnb¹

(Chen et al., 2014)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Avic\GFP^{EGFP.UAS.Tag:PH(hPLCD1)}, Scer\GAL4^nSyb.PS, nwk¹ has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-enhanceable by Df(3R)3450/WASp¹

(Khuong et al., 2010)

Suppressed by

Statement

Reference

Avic\GFP^{EGFP.UAS.Tag:PH(hPLCD1)}, Scer\GAL4^nSyb.PS has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by synj[+]/Synj¹

(Khuong et al., 2010)

Enhancer of

Statement

Reference

Scer\GAL4^nSyb.PS/Avic\GFP^{EGFP.UAS.Tag:PH(hPLCD1)} is an enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of nwk¹

(Khuong et al., 2010)

NOT Enhancer of

Statement

Reference

Scer\GAL4^nSyb.PS/Avic\GFP^{EGFP.UAS.Tag:PH(hPLCD1)} is a non-enhancer of embryonic/larval neuromuscular junction | third instar larval stage | somatic clone phenotype of tweek²/tweek¹

(Khuong et al., 2010)

Scer\GAL4^nSyb.PS/Avic\GFP^{EGFP.UAS.Tag:PH(hPLCD1)} is a non-enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Df(3R)3450/WASp¹

(Khuong et al., 2010)

Suppressor of

Statement

Reference

Scer\GAL4^pnr-MD237/Avic\GFP^{EGFP.UAS.Tag:PH(hPLCD1)} is a suppressor | partially of mechanosensory neuron | adult stage phenotype of PRL-1⁶/PRL-1²

(Urwyler et al., 2019)

Scer\GAL4^pnr-MD237/Avic\GFP^{EGFP.UAS.Tag:PH(hPLCD1)} is a suppressor | partially of neuron projection | adult stage phenotype of PRL-1⁶/PRL-1²

(Urwyler et al., 2019)

Scer\GAL4^nSyb.PS/Avic\GFP^{EGFP.UAS.Tag:PH(hPLCD1)} is a suppressor | partially of NMJ bouton phenotype of mnb¹

(Chen et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\PLCD1^{PH.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^nSyb.PS suppresses the increase in bouton size in mnb¹ mutant NMJs, but does not suppress the reduction in bouton number.

(Chen et al., 2014)

One copy of synj¹ suppresses the neuromuscular junction overgrowth seen when Hsap\PLCD1^{PH.Scer\UAS.T:Avic\GFP-EGFP} is expressed under the control of Scer\GAL4^n-syb.PS in third instar larvae.

Expression of Hsap\PLCD1^{PH.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^n-syb.PS does not enhance the neuromuscular junction overgrowth phenotype seen in tweek¹/tweek² third instar larvae.

Expression of Hsap\PLCD1^{PH.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^n-syb.PS does not enhance the neuromuscular junction overgrowth seen in WASp¹/Df(3R)3450 third instar larvae.

Expression of Hsap\PLCD1^{PH.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^n-syb.PS enhances the overgrown neuromuscular junction phenotype seen in nwk¹ third instar larvae.

Expression of WASp¹/Df(3R)3450 does not enhance the enlarged neuromuscular junction phenotype seen when Hsap\PLCD1^{PH.Scer\UAS.T:Avic\GFP-EGFP} is expressed under the control of Scer\GAL4^n-syb.PS in homozygous nwk¹ third instar larvae.

(Khuong et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

39693

y¹ w^*; L¹/CyO; P{UAS-PLCδ-PH-EGFP}3/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Avic\GFP^{EGFP.UAS.Tag:PH(hPLCD1)}

Hsap\PLCD1^{PH.Scer\UAS.T:Avic\GFP-EGFP}

Name Synonyms

Secondary FlyBase IDs

FBal0241654
FBal0340337

References (26)

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