UASp regulatory sequences drive expression of Hsp67Bc cDNA with a R126E mutation (R126 is a potential pathogenic hot-spot residue corresponding to K141 in Hsap\HSPB8); tagged with Venus.
CGC9371942GAG
CGC>GAG
R126E | Hsp67Bc-PA
R126E
Analogous K141E mutation in human HSPB8 implicated in neuronopathy; mutation carried on in vitro construct.