FlyBase Allele Report: Dmel\hrm[Δ1]

General Information

Symbol

Dmel\hrm^Δ1

Species

D. melanogaster

Name

FlyBase ID

FBal0344639

Feature type

allele

Associated gene

Dmel\hrm

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Velentzas et al., 2018)

Progenitor genotype

Cytology

Description

Deletion of almost all of the predicted hrm open reading frame.

(Velentzas et al., 2018)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:5,641,797..5,644,190 [-]

Comment:

Comment:A 2394bp deletion that removes most of the hrm coding sequence.

(Velentzas and Baehrecke, 2019.3.20)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | pupal stage (with Df(2R)BSC696)

(Velentzas et al., 2018)

fertile

(Velentzas et al., 2018)

increased cell number | pupal stage

(Velentzas et al., 2018)

increased cell number | pupal stage (with Df(2R)BSC696)

(Velentzas et al., 2018)

viable

(Velentzas et al., 2018)

Phenotype Manifest In

autophagosome | pupal stage (with Df(2R)BSC696)

(Velentzas et al., 2018)

lipid droplet | pupal stage (with Df(2R)BSC696)

(Velentzas et al., 2018)

salivary gland | pupal stage

(Velentzas et al., 2018)

salivary gland | pupal stage (with Df(2R)BSC696)

(Velentzas et al., 2018)

Detailed Description

Statement

Reference

hrm^Δ1 homozygous adults do not exhibit any obvious exterior morphological defects.

The majority of hrm^Δ1 homozygous and hrm^Δ1/Df(2R)BSC696 transheterozygous animals exhibit a persistent salivary gland tissue, particularly cell fragments, 24h after puparium formation, i.e. 8hr after the time when this tissue is usually cleared in control individuals; 13.5h after puparium formation, hrm^Δ1/Df(2R)BSC696 transheterozygous salivary glands show a significant decrease in autophagy (i.e. increase in Atg8-positive puncta and converse decrease in Ref(2)p/p62 puncta) and a significant increase in lipid droplet size, but do not show any significant changes in apoptosis (caspase-positive puncta) or in lipid droplet number, as compared to controls.

(Velentzas et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Df(2R)BSC696/hrm^Δ1 has increased cell number | pupal stage phenotype, enhanceable by Scer\GAL4^fkh.PH/BacA\p35^UAS.cUa

(Velentzas et al., 2018)

NOT Enhanced by

Statement

Reference

Df(2R)BSC696/hrm^Δ1 has increased cell number | pupal stage phenotype, non-enhanceable by Atg13^Δ74/Atg13^Δ74

(Velentzas et al., 2018)

Suppressed by

Statement

Reference

Df(2R)BSC696/hrm^Δ1 has increased cell number | pupal stage phenotype, suppressible | partially by Scer\GAL4^fkh.PH/Atg1^GS10797

(Velentzas et al., 2018)

hrm^Δ1 has increased cell number | pupal stage phenotype, suppressible by mTor^TED.UAS/Scer\GAL4^hs.PB

(Velentzas et al., 2018)

NOT suppressed by

Statement

Reference

Df(2R)BSC696/hrm^Δ1 has increased cell number | pupal stage phenotype, non-suppressible by Atg13^Δ74/Atg13^Δ74

(Velentzas et al., 2018)

Other

Statement

Reference

Atg13^Δ74, Scer\GAL4^fkh.PH, hrm^Δ1/hrm[+] has increased cell number | pupal stage phenotype

(Velentzas et al., 2018)

BacA\p35^UAS.cUa, Scer\GAL4^fkh.PH, hrm^Δ1/hrm[+] has increased cell number | pupal stage phenotype

(Velentzas et al., 2018)

Phenotype Manifest In

Enhanced by

Statement

Reference

Df(2R)BSC696/hrm^Δ1 has salivary gland | pupal stage phenotype, enhanceable by Scer\GAL4^fkh.PH/BacA\p35^UAS.cUa

(Velentzas et al., 2018)

NOT Enhanced by

Statement

Reference

Df(2R)BSC696/hrm^Δ1 has salivary gland | pupal stage phenotype, non-enhanceable by Atg13^Δ74/Atg13^Δ74

(Velentzas et al., 2018)

Suppressed by

Statement

Reference

Df(2R)BSC696/hrm^Δ1 has salivary gland | pupal stage phenotype, suppressible | partially by Scer\GAL4^fkh.PH/Atg1^GS10797

(Velentzas et al., 2018)

hrm^Δ1 has salivary gland | pupal stage phenotype, suppressible by mTor^TED.UAS/Scer\GAL4^hs.PB

(Velentzas et al., 2018)

NOT suppressed by

Statement

Reference

Df(2R)BSC696/hrm^Δ1 has salivary gland | pupal stage phenotype, non-suppressible by Atg13^Δ74/Atg13^Δ74

(Velentzas et al., 2018)

Other

Statement

Reference

Atg13^Δ74, Scer\GAL4^fkh.PH, hrm^Δ1/hrm[+] has salivary gland | pupal stage phenotype

(Velentzas et al., 2018)

BacA\p35^UAS.cUa, Scer\GAL4^fkh.PH, hrm^Δ1/hrm[+] has salivary gland | pupal stage phenotype

(Velentzas et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The salivary gland tissue persistence phenotype observed in hrm^Δ1/Df(2R)BSC696 transheterozygous pupae is not affected by Atg13^Δ74 homozygosity; hrm^Δ1 heterozygotes with additional Atg13^Δ74 homozygosity show a salivary gland tissue persistence phenotype.

(Velentzas et al., 2018)

Xenogenetic Interactions

Statement

Reference

The salivary gland tissue persistence phenotype observed in hrm^Δ1/Df(2R)BSC696 transheterozygous pupae is enhanced by the expression of BacA\p35^UAS.cUa under the control of Scer\GAL4^fkh.PH salivary gland tissue, leading to more intact gland fragments 24h after puparium formation; hrm^Δ1 heterozygotes with additional expression of BacA\p35^UAS.cUa under the control of Scer\GAL4^fkh.PH show a salivary gland tissue persistence phenotype.

(Velentzas et al., 2018)

Complementation and Rescue Data

Rescued by

hrm^Δ1 is rescued by hrm^UAS.cVa/Scer\GAL4^hs.PB

(Velentzas et al., 2018)

Comments

The expression of hrm^UAS.cVa under the control of Scer\GAL4^hs.PB, in the absence of heat-shock, suppresses the salivary gland persistence defects observed in hrm^Δ1 homozygotes.

(Velentzas et al., 2018)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

hrm^Delta1

(Velentzas and Baehrecke, 2019.3.20)

hrm^Δ1

(Velentzas et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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