FB2024_03 , released June 25, 2024
Allele: Dmel\AP-2σang-5
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General Information
Symbol
Dmel\AP-2σang-5
Species
D. melanogaster
Name
FlyBase ID
FBal0344946
Feature type
allele
Associated gene
Dmel\AP-2σ
Associated Insertion(s)
P{SUPor-P}AP-2σang-5
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Choudhury et al., 2016)
Mutagen
Delta2-3 transposase
(Choudhury et al., 2016)
Progenitor genotype
Associated Insertion(s)
P{SUPor-P}AP-2σang-5
Cytology
Description

Insertion of a P{SUPor-P} element approximately 136bp upstream of lncRNA:Hsrω. Transcription of lncRNA:Hsrω is unaffected, however, there is a severe reduction in the level of transcription of the AP-2σ gene compared to controls.

(Choudhury et al., 2016)
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Larval muscle 6/7 NMJs in AP-2σang-5/AP-2σang-7 transheterozygotes show significantly more and significantly smaller boutons, which exhibit a significant decrease in periodic microtubule loop structures (Futsch staining), and show a significant decrease in the vesicle pool, as compared to controls. Electrophysiology recording at the larval NMJ of AP-2σang-5/AP-2σang-7 transheterozygotes also show significant increases in mEJP frequency and amplitude, a significant decreases in EJP amplitude and quantal content, and a significant decline in EJP amplitude readings upon high-frequency stimulation at 34[o]C conditions even after a 4min rest period, as compared to controls.

      (Choudhury et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      AP-2σang-7/AP-2σang-5 is partially rescued by Scer\GAL4Act5C.PU/AP-2σUAS.cCa

      (Choudhury et al., 2016)

      AP-2σang-7/AP-2σang-5 is partially rescued by Scer\GAL4elav-C155/AP-2σUAS.cCa

      (Choudhury et al., 2016)
      Comments

      The expression of AP-2σUAS.cCa under the control of Scer\GAL4elav-C155 rescues the synaptic phenotypes but not the lethality observed in AP-2σang-5/AP-2σang-7 transheterozygotes.

      (Choudhury et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      AP-2σang-5
      ang5
      (Choudhury et al., 2016)
      angur5
      (Choudhury et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)