αTub84B sequence which carries the K40A amino acid replacement has been knocked in to the attP site present in the αTub84BKO-attP knockout line.
AAG7087348GCG
The endogenous AAG (lysine) codon was substituted with an GCG (alanine) codon.
αTub84BK40A mutant larvae show significantly decreased responses (rolling) to gentle and harsh touch (at 44mN), and to sound, but not to noxious heat (39.5[o]C), as compared to controls. αTub84BK40A mutant larvae, however, do not exhibit obvious locomotion defects.
In αTub84BK40A mutant larvae, C3da neuron dendrites present significantly more microtubules (Futsch immunostaining) than controls.
αTub84BK40A larval class IV da neurons do not show apparent changes to the polarized dendritic localization of Golgi outposts ( ManII::GFP-labelled ). Retrograde transport of lysosomes in dendrites exhibits a significant decrease in speed but a significant increase in flux, whereas the anterograde transport is not affected, as compared to controls; lysosome transport in axons (both anterograde and retrograde) is not affected. Although the overall dendritic coverage appears normal, there is a progressive increase in the number of terminal branches (increase observed at 120h AEL, but not at 48h AEL), as compared to controls; dendrites show a significantly higher frequency of extension events, despite of no change in de novo branch growth or in retraction events, as compared to controls. There is a severe decrease in growing microtubules (EB1 comets) in dendrites, but not in axons, as compared to controls.
αTub84BK40A has abnormal touch response | larval stage phenotype, non-enhanceable by AtatKO/AtatKO
αTub84BK40A has abnormal touch perception | larval stage phenotype, non-enhanceable by AtatKO/AtatKO
αTub84BK40A has abnormal touch response | larval stage phenotype, suppressible by AtatUAS.L.GFP/Scer\GAL4nompC.PP
αTub84BK40A has abnormal touch perception | larval stage phenotype, suppressible by AtatUAS.L.GFP/Scer\GAL4nompC.PP
αTub84BK40A/αTub84BK40A is a non-enhancer of abnormal touch response | larval stage phenotype of AtatKO
αTub84BK40A/αTub84BK40A is a non-enhancer of abnormal touch perception | larval stage phenotype of AtatKO
αTub84BK40A/αTub84BK40A is a non-enhancer of abnormal touch response | larval stage phenotype of HDAC6KO
αTub84BK40A/αTub84BK40A is a non-enhancer of abnormal touch perception phenotype of HDAC6KO
αTub84BK40A is a suppressor of abnormal touch response | larval stage phenotype of AtatUAS.L.GFP, Scer\GAL4nompC.PP
αTub84BK40A is a suppressor of abnormal touch perception | larval stage phenotype of AtatUAS.L.GFP, Scer\GAL4nompC.PP
αTub84BK40A, αTub84DKO/Df(3R)BSC747 has viable phenotype
αTub84BK40A, αTub84DKO/Df(3R)ED7665 has viable phenotype
αTub84BK40A/Df(3R)BSC465, Df(3R)ED7665/αTub84DKO has viable phenotype