αTub84B^K40A mutant larvae show significantly decreased responses (rolling) to gentle and harsh touch (at 44mN), and to sound, but not to noxious heat (39.5[o]C), as compared to controls. αTub84B^K40A mutant larvae, however, do not exhibit obvious locomotion defects.

In αTub84B^K40A mutant larvae, C3da neuron dendrites present significantly more microtubules (Futsch immunostaining) than controls.

αTub84B^K40A larval class IV da neurons do not show apparent changes to the polarized dendritic localization of Golgi outposts ( ManII::GFP-labelled ). Retrograde transport of lysosomes in dendrites exhibits a significant decrease in speed but a significant increase in flux, whereas the anterograde transport is not affected, as compared to controls; lysosome transport in axons (both anterograde and retrograde) is not affected. Although the overall dendritic coverage appears normal, there is a progressive increase in the number of terminal branches (increase observed at 120h AEL, but not at 48h AEL), as compared to controls; dendrites show a significantly higher frequency of extension events, despite of no change in de novo branch growth or in retraction events, as compared to controls. There is a severe decrease in growing microtubules (EB1 comets) in dendrites, but not in axons, as compared to controls.

αTub84B^K40A has abnormal touch response | larval stage phenotype, non-enhanceable by Atat^KO/Atat^KO

αTub84B^K40A has abnormal touch perception | larval stage phenotype, non-enhanceable by Atat^KO/Atat^KO

αTub84B^K40A has abnormal touch response | larval stage phenotype, suppressible by Atat^UAS.L.GFP/Scer\GAL4^nompC.PP

αTub84B^K40A has abnormal touch perception | larval stage phenotype, suppressible by Atat^UAS.L.GFP/Scer\GAL4^nompC.PP

αTub84B^K40A/αTub84B^K40A is a non-enhancer of abnormal touch response | larval stage phenotype of Atat^KO

αTub84B^K40A/αTub84B^K40A is a non-enhancer of abnormal touch perception | larval stage phenotype of Atat^KO

αTub84B^K40A/αTub84B^K40A is a non-enhancer of abnormal touch response | larval stage phenotype of HDAC6^KO

αTub84B^K40A/αTub84B^K40A is a non-enhancer of abnormal touch perception phenotype of HDAC6^KO

αTub84B^K40A is a suppressor of abnormal touch response | larval stage phenotype of Atat^UAS.L.GFP, Scer\GAL4^nompC.PP

αTub84B^K40A is a suppressor of abnormal touch perception | larval stage phenotype of Atat^UAS.L.GFP, Scer\GAL4^nompC.PP

αTub84B^K40A, αTub84D^KO/Df(3R)BSC747 has viable phenotype

αTub84B^K40A, αTub84D^KO/Df(3R)ED7665 has viable phenotype

αTub84B^K40A/Df(3R)BSC465, Df(3R)ED7665/αTub84D^KO has viable phenotype