FlyBase Allele Report: Dmel\Atat[KO]

General Information

Symbol

Dmel\Atat^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0345149

Feature type

allele

Associated gene

Dmel\Atat

Associated Insertion(s)

TI{TI}Atat^KO

Carried in Construct

Also Known As

dTat^KO

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Yan et al., 2018)

Mutagen

CRISPR/Cas9

(Yan et al., 2018)

gene targeting by homologous recombination

(Yan et al., 2018)

Progenitor genotype

Associated Insertion(s)

TI{TI}Atat^KO

Cytology

Description

869bp deletion that deletes the first three coding exons shared by all Tat isoforms. A single loxP site is present at the site of the deletion.

(Yan et al., 2018)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

germarium

(Antel et al., 2022)

larval multidendritic class III neuron | larval stage

(Yan et al., 2018)

larval multidendritic class III neuron | larval stage | conditional

(Yan et al., 2018)

larval multidendritic class IV neuron | larval stage

(Yan et al., 2018)

microtubule | larval stage

(Yan et al., 2018)

microtubule | larval stage | conditional

(Yan et al., 2018)

Detailed Description

Statement

Reference

Atat^KO adults exhibit a reduction in their climbing ability.

(Dobbelaere et al., 2020)

Tat^KO and Tat^KO/Df(3L)BSC113 mutant larvae show a significantly decreased response to gentle touch, as compared to controls; Tat^KO mutant larvae also show significantly decreased responses (rolling) to harsh touch (at 11mN to 44mN, but not 78mN to 127mN) and to vibration (70dB, 500Hz sound), but not to noxious heat (39.5[o]C), as compared to controls; Tat^KO mutant larvae, however, do not exhibit obvious locomotion defects.

Tat^KO mutant larvae do not show any obvious dendrite branch distribution defects in C3da (ddaA, ddaF) and chordotonal neurons; C4da neurons do not show overt dendrite coverage defects but show a minor but significant decrease in branch points, as compared to controls. C3da neuron dendrites present significantly more growing plus end microtubule ends (i.e EB1-GFP comets) and significantly more microtubules (Futsch immunostaining) than controls; mechanostimulation of these neurons also leads to a significant increase in growing plus end microtubule ends in dendrites, which is not observed in controls.

(Yan et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Atat^KO has abnormal touch response | larval stage phenotype, enhanceable by nompC¹/nompC³

(Yan et al., 2018)

Atat^KO has abnormal touch perception | larval stage phenotype, enhanceable by nompC¹/nompC³