FlyBase Allele Report: Dmel\lky[KO]

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Allele: Dmel\lky^KO

General Information

Symbol

Dmel\lky^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0385572

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Antel et al., 2022)

Mutagen

gene targeting by homologous recombination

(Antel et al., 2022)

(Antel et al., 2022)

Progenitor genotype

Associated Insertion(s)

Cytology

Description

The entire lky coding region has been deleted and replaced by a sequence cassette that encodes 1. a Disc\RFP^{DsRed(T1).3xP3.cHa} marker flanked by loxP sites and 2. a single attP site.

(Antel et al., 2022)

Allele components

Component

Use(s)

Also carries

integrase target site

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

X:20,001,069..20,002,125 [-]

Comment:

Deletion of the entire lky coding region. Position of deletion endpoints on reference sequence determined from reported primer sequences used to generate homology arms.

(Antel et al., 2022)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

(Antel et al., 2022)

(Antel et al., 2022)

Phenotype Manifest In

(Antel et al., 2022)

egg chamber (with Df(1)BSC586)

(Antel et al., 2022)

female germline cyst

(Antel et al., 2022)

follicle cell (with Df(1)BSC586)

(Antel et al., 2022)

(Antel et al., 2022)

oocyte | increased number

(Antel et al., 2022)

stalk follicle cell

(Antel et al., 2022)

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

lky^KO has egg chamber phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/αTub84B^K40Q.UAS.GFP

(Antel et al., 2022)

lky^KO has egg chamber phenotype, suppressible | partially by nanos^BN/nanos^L7

(Antel et al., 2022)

NOT suppressed by

Statement

Reference

lky^KO has egg chamber phenotype, non-suppressible by αTub84B^K40Q.UAS.GFP/Scer\GAL4^tj.PU

(Antel et al., 2022)

lky^KO has egg chamber phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/αTub84B^K40R.UAS.GFP

(Antel et al., 2022)

lky^KO has egg chamber phenotype, non-suppressible by αTub84B^K40R.UAS.GFP/Scer\GAL4^tj.PU

(Antel et al., 2022)

Other

Statement

Reference

Atat^KO, lky^KO has germarium phenotype

(Antel et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

lky^KO is rescued by Scer\GAL4^{VP16.nanos.UTR}/lky^UAS.GFP

(Antel et al., 2022)

Df(1)BSC586/lky^KO is rescued by Scer\GAL4^{VP16.nanos.UTR}/lky^UAS.GFP

(Antel et al., 2022)

Not rescued by

lky^KO is not rescued by lky^UAS.GFP/Scer\GAL4^tj.PU

(Antel et al., 2022)

Df(1)BSC586/lky^KO is not rescued by lky^UAS.GFP/Scer\GAL4^tj.PU

(Antel et al., 2022)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

604587

y² VhaAC39-1^cho-2 v¹ TI{TI}lky^KO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

lky^KO

(Antel et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections