FB2024_03 , released June 25, 2024
Allele: Dmel\NinUAS.mEosFP
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General Information
Symbol
Dmel\NinUAS.mEosFP
Species
D. melanogaster
Name
FlyBase ID
FBal0346146
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Progenitor genotype
Carried in construct
Cytology
Description

UASt regulatory sequences drive expression of the Bsg25D-PB isoform (sequence derived from the LD21844 cDNA clone). The coding sequence is tagged at the N-terminal end with mEosFP.

Allele components
Component
Use(s)
Encoded product / tool
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing Bsg25DUAS.mEosFP under the control of either Scer\GAL4Mef2.PR or Scer\GAL4kirre-rP298-G4 results in stage 16 embryo myotubes showing abnormal nuclear positioning, namely nuclei clustering, and nuclei spreading/traversing a shorter distance toward the muscle poles, as compared to controls; the number of nuclei per muscle cells remains unchanged.

Larvae expressing Bsg25DUAS.mEosFP under the control of Scer\GAL4Mef2.PR present a significant decrease in average crawling speed, as compared to controls. Their muscles exhibit a significant decrease in stiffness, exhibit smaller and clustered nuclei, and exhibit severe microtubule organization defects (longitudinal microtubules are present, but peri-nuclear microtubule arrays are completely absent), as compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Bsg25DUAS.mEosFP
NinUAS.mEosFP
Name Synonyms
Secondary FlyBase IDs
    References (1)