FlyBase Allele Report: Dmel\Rdl[MD-RR]

General Information

Symbol

Dmel\Rdl^MD-RR

Species

D. melanogaster

Name

FlyBase ID

FBal0351976

Feature type

allele

Associated gene

Dmel\Rdl

Associated Insertion(s)

Carried in Construct

Also Known As

MD-RR

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

natural population

Nature of the Allele

Allele class

Mutagen

natural population

(Zhang et al., 1994, ffrench-Constant et al., 1993, ffrench-Constant et al., 1993, ffrench-Constant and Roush, 1991, ffrench-Constant et al., 1990)

Progenitor genotype

Cytology

Description

Rdl single copy line (does not contain the Dp(3;3)Rdl duplication).

(Remnant et al., 2013)

The lesion in the Rdl^MD-RR allele has been described as A302S in print, but the amino acid replacement is actually A301S.

(Stilwell, 2011.4.18)

Amino acid replacement: A301S.

(Stilwell, 2011.4.18)

Amino acid replacement: A302S. There are no other amino acid replacements in this allele.

(Zhang et al., 1994)

Amino acid replacement: A302S.

(ffrench-Constant et al., 1993, ffrench-Constant et al., 1993)

Nucleotide substitution: G995T.

(ffrench-Constant et al., 1993)

Mutation falls within the second membrane spanning region of the protein (a region which is highly conserved among GABA[[A]] receptor genes).

(ffrench-Constant et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:9,155,164..9,155,164 [-]

Nucleotide change:

G9155164T

Reported nucleotide change:

G995T

Amino acid change:

Reported amino acid change:

A301S

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Stilwell, 2011.4.18, ffrench-Constant et al., 1993)

point_mutation

3L:9,151,833..9,151,833 [-]

Nucleotide change:

G9151833H

Amino acid change:

Reported amino acid change:

M361I

Comment:

Two missense mutations in strain. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(ffrench-Constant et al., 1993)

point_mutation

3L:9,155,164..9,155,164 [-]

Nucleotide change:

G9155164T

Amino acid change:

Reported amino acid change:

A302S

Comment:

Two missense mutations in strain. The Ala-Ser mutation thought to be associated with PTX/cyclodiene insensitivity. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(ffrench-Constant et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Mutant larvae are more resistant to fipronil and to pyriprole compared to wild type.

(Remnant et al., 2014)

Mutant flies show more than 45,000-fold resistance to dieldrin compared to wild type.

Mutant flies are significantly slower to recover from heat shock compared to wild type.

(Remnant et al., 2013)

Rdl^MD-RR mutants show behavioral convulsions after brief exposure to elevated temperature.

(Hoeffer et al., 2003)

Rdl^MD-RR neurons have a marked reduction in sensitivity to the picrotoxin blockade of spontaneous GABAergic postsynaptic currents compared to wild type. Additionally, there is a much lower increase in neuron excitability in response to picrotoxin in the Rdl^MD-RR mutants compared to wild type.

(Lee et al., 2003)

Mutant flies are resistant to dieldrin (more than 55-fold resistant) and α-thujone (5-fold resistant) compared to wild-type flies.

(Hold et al., 2000)

Resistant to cyclodiene. Reduces the single channel conductance by 5% for inward current and 17% for outward current. Single channel current is approximately 60% lower for outward current than for inward current in both wild type and mutant. Increases open time and reduces closed times, reflecting a net stabilization of the channel open state by a factor of approximately five. Shows a marked reduction in the rate of GABA-induced desensitization, and a net destabilization of the desensitized conformation by a factor of 29.

(Zhang et al., 1994)

Confers resistance to PTX and cyclodienes.

(ffrench-Constant et al., 1993)

Resistant to dieldrin selection.

(ffrench-Constant et al., 1993)

Flies are insensitive to 10μM dieldrin, and less sensitive to picrotoxinin than wild-type.

(Bloomquist et al., 1991)

Flies show approximately 4000-fold resistance to dieldrin compared to wild-type.

(ffrench-Constant et al., 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Rdl^MD-RR is a suppressor of abnormal sleep phenotype of Nlg4^LL01874

(Li et al., 2013)

Rdl[+]/Rdl^MD-RR is a suppressor of paralytic | recessive phenotype of kcc^DHS1

(Hekmat-Scafe et al., 2010)

NOT Suppressor of

Statement

Reference

Rdl[+]/Rdl^MD-RR is a non-suppressor of lethal - all die before end of P-stage phenotype of Adar^3.4.S.UAS, Scer\GAL4^Act5C.PI

(Li et al., 2014)

Other

Statement

Reference

Rdl^MD-RR, smi26D¹/smi26D[+] has abnormal smell perception | dominant | female phenotype

(Anholt et al., 2003)

Rdl^MD-RR, smi26D¹ has abnormal smell perception | dominant | female phenotype

(Anholt et al., 2003)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Rdl^MD-RR/+ does not rescue the lethality of flies expressing Adar^{3.4.S.Scer\UAS} under the control of Scer\GAL4^Act5C.PI.

(Li et al., 2014)

The introduction of a Rdl^R-VL background successfully restores night sleep in Nlg4^LL01874 mutants, indicating that the sleep defects observed in Nlg4^LL01874 mutants are due to impaired GABA transmission.

(Li et al., 2013)

The bang-sensitive paralytic phenotype of homozygous kcc^DHS1 mutants is significantly suppressed by Rdl^MD-RR/+.

(Hekmat-Scafe et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (4)

1675

35492

29636

106444

Notes on Origin

Discoverer

Isolated from: Maryland, USA population.

(ffrench-Constant and Roush, 1991)

Comments

The A302S substitution is found in 14 resistant strains of D.melanogaster. The M361I amino acid replacement is found in some, but not all, resistant strains tested so is likely to be a polymorphism unrelated to insecticide resistance.

(ffrench-Constant et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

MD-RR

(ffrench-Constant and Roush, 1991, ffrench-Constant et al., 1990)

Rdl^A2'S

(Guo et al., 2023)

Rdl^A302S

(Li et al., 2013)

Rdl^MD-RR

(Mi et al., 2023, Molina-Obando et al., 2019, Li et al., 2014, Stilwell, 2011.4.18, Hekmat-Scafe et al., 2010, Hoeffer et al., 2003, ffrench-Constant et al., 1993)

Rdl^R-MD

Rdl^R-VL

Rdl^R MD-RR

(Remnant et al., 2013)

Rdl^R-MDRR

(Remnant et al., 2014)

Rdl^ts

(Hoeffer et al., 2003)

Name Synonyms

Resistant

(ffrench-Constant et al., 1993)

Secondary FlyBase IDs

FBal0290533
FBal0030108
FBal0030109
FBal0052119

References (21)

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