FB2024_03 , released June 25, 2024
Allele: Dmel\mAChR-AKK
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General Information
Symbol
Dmel\mAChR-AKK
Species
D. melanogaster
Name
FlyBase ID
FBal0371824
Feature type
allele
Associated gene
Dmel\mAChR-A
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Rozenfeld et al., 2021)
gene targeting by homologous recombination
(Rozenfeld et al., 2021)
Progenitor genotype
Cytology
Description

Amino acid replacement: D301K.

(Rozenfeld et al., 2021)

Amino acid replacement: P303K.

(Rozenfeld et al., 2021)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
MNV
2R:24,383,971..24,383,979 [-]
Inserted_sequence:

AAGCTGAAG

Comment:

Codons D301 and P303 of mAChR-A were replaced by K codons (AAG) via CRISPR. Silent point mutations were also introduced in the PAM region of the guide RNA targets.

(Rozenfeld et al., 2021)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Unlike controls, in mAChR-AKK antennal lobe inhibitory local neurons (ILNs), muscarine-evoked currents are not voltage-dependent; unlike controls, a depolarizing pre-pulse step (40 mV, 30 sec) does not potentiated the response to muscarine. ILNs show voltage-independent post-tetanic potentiation and abolished nicotine-induced potentiation. Unlike controls, ILNs do not show a decrease in firing rate upon exposure to the odor (ethyl butyrate); adult show significantly stronger habituation to ethyl butyrate and apple cider vinegar in olfactory avoidance/attraction assays.

      (Rozenfeld et al., 2021)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      mAChR-A-KK
      (Rozenfeld et al., 2021)
      mAChR-AKK
      Name Synonyms
      Secondary FlyBase IDs
        References (1)