cdc25, cdc25string, l(3)01235, string/cdc25, EP1213
protein tyrosine phosphatase - activates cyclin dependent kinase causing mitotic entry - the switch-like entry into mitosis observed in the Drosophila embryo during the 14th mitotic cycle is timed by the dynamics of Cdc25(String) accumulation
Please see the JBrowse view of Dmel\stg for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.47
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.55
2.8 (northern blot)
479 (aa)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\stg using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: maternally deposited
During Malpighian tubule development, stg is expressed asymmetrically in everting tubules, and subsequently in the distal proliferation zone.
stg transcripts are expressed maximally in nurse cells in the later stages of oogenesis and the transcripts are transferred to the oocyte from stage 11 onward. The maternally derived transcripts are uniformly distributed in the cytoplasm of the early embryo and are excluded from the forming cells during cellularization. Zygotic expression of stg initiates at cycle 14 and has been described elsewhere (FBrf 49340). stg transcripts are expressed in a variety of proliferating cells and are shown in a broad band that anticipates the mitotic divisions as the morphogenetic furrow passes aacross the eye-entennal disc and in proliferating cells in the optic lobes of the brain. stg transcripts are also expressed in the apical cells of the male testis.
stg transcripts are most abundant in early embryos and adult females.
JBrowse - Visual display of RNA-Seq signals
View Dmel\stg in JBrowse
3-98
3-94.8
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
Expression is enriched in embryonic gonads.
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: cell size is increased, microtubules are uniform or disorganised, cell shape is irregular, and cell number is decreased indicative of a failure in cell cycle progression through G1 to S and G2 to M stages.
The joint action of two RNA degradation pathways (a maternally encoded and a zygotic pathway) controls maternal transcript degradation and its timing in the early embryo. stg transcripts (relatively high in abundance) require the action of both pathways in order to be eliminated prior to the midblastula transition.
Many modular elements with separable activities, spread over more than 30kb, control stg transcription in the embryo and imaginal discs.
stg is required for completion of daughter centriole assembly in embryos.
Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.
Identification: Enhancer trap screen designed to discover genes involved in the cellular aspects of defense mechanisms, as well as in melanotic tumor formation processes linked to blood cell disregulation.
The coordinate program of expression of S phase genes (DNApol-α180, mus209, RnrL and RnrS) is not disrupted in stg mutants and is therefore not a secondary consequence of cell cycle progression.
Known patterning genes act locally to influence stg transcription. Complete pattern of stg transcription requires >15.3kb of cis-acting regulatory sequences. stg transcription is largely unaffected in mutant embryos arrested in G2 of cycles 14, 15 or 16, or G1 of cycle 17. Thus there is a regulatory hierarchy in which developmental inputs, not cell cycle inputs, control the timing of stg transcription and hence cell cycle progression. Overall orientation not stated: anon-99A? stg+ ptls+
Mutants are embryonic lethal; denticle bands are reduced. First 13 nuclear divisions of zygote proceed on schedule; division 14 permanently arrested in G2 in homozygotes for strong alleles; no evidence of nuclear-envelope breakdown or chromosome condensation; division 14 severely impaired in weak alleles. Gastrulation proceeds on schedule in stg mutant embryos with but 5,000 cells. The only defect seems to be in the initiation of the first mitotic division that is under zygotic control. Clones of homozygous cells induced early don't survive; few late clones produced.
Mutations in zygotic gene stg do not interact with RpII140wimp.
The effects of an altered nucleocytoplasmic ratio on transcripts that normally undergo changes in transcript pattern in cell cycle 14 is studied. A delay in the maternal-to-zygotic transition of the mitotic control gene stg is correlated with a decrease in nuclear density and a change in the cell cycle program.
The normal number of cell divisions is important to achieve proper cuticular differentiation, whereas the relative timing of these divisions is less critical.
stg gene product activity is rate limiting for the cell cycle transition G2/M during embryonic cell cycles 14, 15 and 16.
Regulated expression of stg mRNA controls the timing and location of zygotically driven embryonic cell divisions.
stg mutants display a strong reduction in the number of denticle rows.
Source for merge of: stg EP1213
P{EP} insertion in "EP1213EP1213" is approximately 1.5kb upstream of the stg transcription unit. However, it is not clear whether stg or a gene other than stg is affected in the P{EP}stgEP1213 insertion line.
"l(3)0674306743" may be a leaky allele of "stg".
"Dr" mutants are always double hits in two genes, one of which is "stg".
Interactions between "Dr" revertant alleles and "stg" demonstrate they are separate loci.
Can rescue allele 22 of Spom\cdc25, but not a deletion of the gene.
Source for identity of: stg CG1395
