FlyBase Gene Report: Dmel\Pi3K59F

General Information

Symbol

Dmel\Pi3K59F

Species

D. melanogaster

Name

Phosphatidylinositol 3-kinase 59F

Annotation Symbol

CG5373

Feature Type

protein_coding_gene

FlyBase ID

FBgn0015277

Gene Model Status

Current

Stock Availability

17 publicly available

Enzyme Name (EC)

phosphatidylinositol 3-kinase (2.7.1.137)

Gene Summary

Phosphotidylinositol 3 kinase 59F (Pi3K59F) encodes a lipid kinase that is required for formation of Phosphatidylinositol 3-phosphate. It functions in a core protein complex that includes the products of Atg6 and Vps15. It is involved in the control of vesicle trafficking, including autophagy, endocytosis and phagocytosis. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Vps34, PI3K, dPI3K, PI3K_59F, PI3K-59F

Key Links

Genomic Location

Cytogenetic map

59E4-59F1

Sequence location

2R:23,560,492..23,564,045 [+]

Recombination map

2-103

RefSeq locus

NT_033778 REGION:23560492..23564045

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (30 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables 1-phosphatidylinositol-3-kinase activity

inferred from direct assay

(Linassier et al., 1997)

NOT enables 1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity

inferred from direct assay

(Linassier et al., 1997)

NOT enables 1-phosphatidylinositol-4-phosphate 3-kinase activity

inferred from direct assay

(Linassier et al., 1997)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables 1-phosphatidylinositol-3-kinase activity

inferred from sequence or structural similarity with SGD:S000004230

(Vanhaesebroeck et al., 1997)

inferred from electronic annotation with InterPro:IPR008290

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000005955

(GO Reference Genome Project, 2011-)

Biological Process (16 terms)

Terms Based on Experimental Evidence (13 terms)

CV Term

Evidence

References

involved_in autophagy

inferred from mutant phenotype

(Wen et al., 2017, Xu et al., 2015, Chang et al., 2013, Juhász et al., 2008)

inferred from genetic interaction with FLYBASE:Atg6; FB:FBgn0264325

(Juhász et al., 2008)

involved_in cellular response to starvation

inferred from mutant phenotype

(Juhász et al., 2008)

involved_in dsRNA transport

inferred from mutant phenotype

(Saleh et al., 2006)

involved_in endocytosis

inferred from mutant phenotype

(Juhász et al., 2008)

involved_in epithelial structure maintenance

inferred from genetic interaction with FLYBASE:bsk; FB:FBgn0000229

(O'Farrell et al., 2017)

inferred from genetic interaction with FLYBASE:Lkb1; FB:FBgn0038167

(O'Farrell et al., 2017)

involved_in establishment or maintenance of cell polarity

inferred from genetic interaction with FLYBASE:Lkb1; FB:FBgn0038167

(O'Farrell et al., 2017)

involved_in larval midgut cell programmed cell death

inferred from mutant phenotype

(Xu et al., 2015, Chang et al., 2013)

involved_in neuron remodeling

inferred from mutant phenotype

(Issman-Zecharya and Schuldiner, 2014)

involved_in phosphatidylinositol phosphate biosynthetic process

inferred from direct assay

(Linassier et al., 1997)

involved_in positive regulation of nurse cell apoptotic process

inferred from mutant phenotype

(Nezis et al., 2010)

involved_in protein targeting to lysosome

inferred from mutant phenotype

(Csizmadia et al., 2018)

involved_in regulation of autophagy

inferred from mutant phenotype

(Pircs et al., 2012)

involved_in regulation of early endosome to late endosome transport

inferred from mutant phenotype

(O'Farrell et al., 2017)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in autophagosome assembly

inferred from biological aspect of ancestor with PANTHER:PTN000005955

(GO Reference Genome Project, 2011-)

involved_in endocytosis

inferred from biological aspect of ancestor with PANTHER:PTN000005955

(GO Reference Genome Project, 2011-)

involved_in phosphatidylinositol phosphate biosynthetic process

inferred from electronic annotation with InterPro:IPR008290, InterPro:IPR015433

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with SGD:S000004230

(Vanhaesebroeck et al., 1997)

involved_in phosphatidylinositol-3-phosphate biosynthetic process

inferred from biological aspect of ancestor with PANTHER:PTN000005955

(GO Reference Genome Project, 2011-)

involved_in phosphatidylinositol-mediated signaling

inferred from biological aspect of ancestor with PANTHER:PTN000005673

(GO Reference Genome Project, 2011-)

Cellular Component (11 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

located_in autophagosome membrane

inferred from direct assay

(Juhász et al., 2008)

located_in early endosome

inferred from direct assay

(Juhász et al., 2008)

located_in omegasome

inferred from mutant phenotype

(Melani et al., 2017)

part_of phosphatidylinositol 3-kinase complex, class III, type I

inferred from direct assay

(Juhász et al., 2008)

Terms Based on Predictions or Assertions (8 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000005673

(GO Reference Genome Project, 2011-)

is_active_in endosome

inferred from biological aspect of ancestor with PANTHER:PTN000005955

(GO Reference Genome Project, 2011-)

is_active_in membrane

inferred from biological aspect of ancestor with PANTHER:PTN000005673

(GO Reference Genome Project, 2011-)

is_active_in peroxisome

inferred from biological aspect of ancestor with PANTHER:PTN000005955

(GO Reference Genome Project, 2011-)

is_active_in phagophore assembly site

inferred from biological aspect of ancestor with PANTHER:PTN000005955

(GO Reference Genome Project, 2011-)

part_of phosphatidylinositol 3-kinase complex, class III, type II

inferred from biological aspect of ancestor with PANTHER:PTN000005955

(GO Reference Genome Project, 2011-)

part_of phosphatidylinositol 3-kinase complex, class III, type I

inferred from biological aspect of ancestor with PANTHER:PTN000005955

(GO Reference Genome Project, 2011-)

part_of phosphatidylinositol 3-kinase complex, class III

non-traceable author statement

(Vanhaesebroeck et al., 1997)

Gene Group (FlyBase)

AUTOPHAGY-SPECIFIC PHOSPHATIDYLINOSITOL 3-KINASE COMPLEX

PHOSPHATIDYLINOSITOL 3 KINASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

1-phosphatidylinositol-3-kinase activity

a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl- sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ADP + H(+) (2.7.1.137)

RHEA 12709:

Summaries

Gene Snapshot

Gene Group (FlyBase)

AUTOPHAGY-SPECIFIC PHOSPHATIDYLINOSITOL 3-KINASE COMPLEX -: The autophagy-specific phosphatidylinositol 3-kinase complex generates PI3-phosphate (PI3P) at the PAS (phagophore assembly site/preautophagosomal structure). (Adapted from FBrf0219844 and PMID:23999079).
PHOSPHATIDYLINOSITOL 3 KINASES -: Phosphatidylinositol 3 kinases catalyze the phosphorylation of phosphatidylinositol (PI), or specific mono or bisphosphate derivatives of PI, at position 3 on the inositol ring to generate PI3P, PI(3,4)P[[2]] or PI(3,4,5)P[[3]]. The resulting phosphoinositides can act as signalling molecules to mediate/regulate a range of cellular and physiological processes, including cell polarity, cell migration, autophagy, growth, development, and ageing. (Adapted from FBrf0227909.)

Summary (Interactive Fly)

regulates intracellular vesicular transport of proteins at distinct membrane domains through production of phosphatidylinositol-3-phosphate - interacts with Rabenosyn-5 which carries out a subset of Pi3K59 functions - promotes autophagy and endocytosis

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Pi3K59F for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W1M7)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

Comments on Gene Model

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Pi3K59F-RA

FBtr0072064

NM_057785

3164

949

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Pi3K59F-PA

FBpp0071973

107.9

949

7.82

Q9W1M7

NP_477133

AAF47030

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pi3K59F using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pi3K59F_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.31

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in autophagosome membrane

inferred from direct assay

(Juhász et al., 2008)

located_in early endosome

inferred from direct assay

(Juhász et al., 2008)

located_in omegasome

inferred from mutant phenotype

(Melani et al., 2017)

part_of phosphatidylinositol 3-kinase complex, class III, type I

inferred from direct assay

(Juhász et al., 2008)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Pi3K59F in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Pi3K59F

View exonic expression by tissue for Dmel\Pi3K59F

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015277

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37733/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015277

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015277

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015277

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5373

Flygut - An atlas of the Drosophila adult midgut

FBgn0015277

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 21 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Pi3K59F

Transgenic constructs containing regulatory region of Pi3K59F

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2R)Exel7180

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal (with Df(2R)bw5)

viable

Pi3K59F^e03961

Sterility

Allele

fertile

Pi3K59F^e03961

Other Phenotypes

Allele

abnormal cell migration | somatic clone

Pi3K59F^Δm22

abnormal cell polarity | somatic clone

Pi3K59F^Δm22

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^Ddc.PL

Pi3K59F^KK107602

abnormal neuroanatomy | adult stage | somatic clone

Pi3K59F^Δm22

abnormal size | P-stage, with Scer\GAL4^NP0001

Pi3K59F^HMS00261

abnormal starvation stress response | larval stage, with Scer\GAL4^Cg.PU

Pi3K59F^HMS00261

abnormal starvation stress response | late third instar larval stage, with Scer\GAL4^Cg.PA

Pi3K59F^KD.UAS

abnormal wound healing | adult stage, with Scer\GAL4^repo

Pi3K59F^KK107602

decreased cell death | P-stage, with Scer\GAL4^NP0001

Pi3K59F^HMS00261

increased cell size, with Scer\GAL4^NP0001

Pi3K59F^KK107602

short lived, with Scer\GAL4^Ddc.PL

Pi3K59F^KK107602

visible, with Scer\GAL4^ap-md544

Pi3K59F^KD.UAS

visible | adult stage, with Scer\GAL4^GMR.PU

Pi3K59F^KK107602

Phenotype manifest in

Allele

adult fat body | nutrition conditional, with Scer\GAL4^Tub.PU

Pi3K59F^KK107602

adult marginal nerve wrapping glial cell, with Scer\GAL4^repo

Pi3K59F^KK107602

adult midgut epithelium, with Scer\GAL4^NP0001

Pi3K59F^KK107602

adult midgut epithelium, with Scer\GAL4^Tub.PU

Pi3K59F^KK107602

autophagosome | adult stage | nutrition conditional, with Scer\GAL4^Tub.PU

Pi3K59F^KK107602

autophagosome | conditional | somatic clone

Pi3K59F^Δm22

autophagosome | conditional | somatic clone, with Scer\GAL4^Act5C.PP

Pi3K59F^KD.UAS

autophagosome | somatic clone

Pi3K59F^Δm22

embryonic/larval fat body | conditional | somatic clone

Pi3K59F^Δm22

embryonic/larval fat body | larval stage, with Scer\GAL4^Cg.PU

Pi3K59F^HMS00261

embryonic/larval fat body | late third instar larval stage, with Scer\GAL4^Cg.PA

Pi3K59F^KD.UAS

embryonic/larval fat body | somatic clone

Pi3K59F^Δm22

embryonic/larval fat body | somatic clone | third instar larval stage, with Scer\GAL4^Act5C.PP

embryonic/larval fat body | third instar larval stage

Pi3K59F^Δm22

embryonic/larval midgut | P-stage, with Scer\GAL4^NP0001

Pi3K59F^HMS00261

eye, with Scer\GAL4^GMR.PU

Pi3K59F^KK107602

eye disc | somatic clone

Pi3K59F^Δm22

follicle cell | somatic clone

Pi3K59F^Δm22

gamma Kenyon cell | adult stage | somatic clone

Pi3K59F^Δm22

garland cell | somatic clone

Pi3K59F^Δm22

garland cell | somatic clone, with Scer\GAL4^Act5C.PP

Pi3K59F^KD.UAS

gastric caecum | P-stage, with Scer\GAL4^NP0001

Pi3K59F^HMS00261

lysosome | adult stage | nutrition conditional, with Scer\GAL4^Tub.PU

Pi3K59F^KK107602

lysosome | conditional | somatic clone

Pi3K59F^Δm22

lysosome | conditional | somatic clone, with Scer\GAL4^Act5C.PP

Pi3K59F^KD.UAS

marginal nerve, with Scer\GAL4^repo

Pi3K59F^KK107602

nurse cell | germline clone | oogenesis stage S14

Pi3K59F^Δm22

ommatidium, with Scer\GAL4^GMR.PU

Pi3K59F^KK107602

phagophore assembly site membrane | conditional | somatic clone, with Scer\GAL4^Act5C.PP

Pi3K59F^KD.UAS

wing, with Scer\GAL4^ap-md544

Pi3K59F^KD.UAS

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (21)

Hsap\PIK3C3

14 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\MTOR

1 of 14

Hsap\PI4KAP1

1 of 14

Yes

Hsap\PI4KAP2

1 of 14

Hsap\SH3PXD2A

1 of 14

Yes

Hsap\SH3PXD2B

1 of 14

Yes

Hsap\SMG1

1 of 14

Hsap\TRRAP

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (13)

Rnor\Pik3c3

14 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (17)

Mmus\Pik3c3

14 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Sh3pxd2b

1 of 14

Yes

Mmus\Smg1

1 of 14

Mmus\Trrap

1 of 14

Xenopus tropicalis (Western clawed frog) (11)

Xtro\pik3c3

7 of 13

Yes

Xtro\pik3ca

2 of 13

Xtro\alox12b

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\slc25a28

1 of 13

Danio rerio (Zebrafish) (15)

Drer\pik3c3

14 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\vps-34

13 of 14

Yes

3 of 14

3 of 14

2 of 14

2 of 14

Anopheles gambiae (African malaria mosquito) (11)

Agam\AgaP_AGAP010273

12 of 12

Yes

3 of 12

3 of 12

3 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (10)

Atha\VPS34

13 of 13

Yes

3 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (7)

Scer\VPS34

13 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (8)

Spom\pik3

12 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Pi3K59F. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (8)

fwd

5 of 13

5 of 13

5 of 13

5 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Pi3K59F^KK107602

exacerbates Parkinson's disease

modeled by Hsap\SNCA^UAS.cFa

(M'Angale and Staveley, 2016)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PIK3C3; phosphatidylinositol 3-kinase catalytic subunit type 3

14 of 14

602609

PIK3C2A; phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha

3 of 14

603601

OCULOSKELETODENTAL SYNDROME; OCSKD

PIK3C2B; phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta

3 of 14

602838

PIK3C2G; phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 gamma

3 of 14

609001

PIK3CD; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta

3 of 14

602839

immunodeficiency 14

PIK3CG; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma

3 of 14

601232

IMMUNODEFICIENCY 97 WITH AUTOINFLAMMATION; IMD97

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

MIST Molecular Interaction Search Tool

View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Pi3K59F - Atg14

anti tag coimmunoprecipitation, anti tag western blot

(Xu et al., 2022)

Pi3K59F - Miga

anti tag coimmunoprecipitation, anti tag western blot

(Xu et al., 2022)

Pi3K59F - Rab5

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Pi3K59F - zda

pull down, autoradiography

(Melani et al., 2017)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Pi3K59F

suppressible

bsk

(O'Farrell et al., 2017)

Pi3K59F

enhanceable

Buffy

(M'Angale and Staveley, 2016)

Pi3K59F

suppressible

Buffy

(M'Angale and Staveley, 2016)

Pi3K59F

suppressible

Lkb1

(O'Farrell et al., 2017)

Pi3K59F

enhanceable

Rab5

(O'Farrell et al., 2017)

Pi3K59F

enhanceable

Rab7

(O'Farrell et al., 2017)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Myc

suppressible

Pi3K59F

(Nagy et al., 2013)

Tsc1|gig

suppressible

Pi3K59F

(Juhász et al., 2008)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

63333

DroID - A comprehensive database of gene and protein interactions.

FBgn0015277

MIST (protein-protein) - An integrated Molecular Interaction Database

37733

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

3-phosphoinositide biosynthesis

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P91635

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-103

Cytogenetic map

59E4-59F1

Sequence location

2R:23,560,492..23,564,045 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

59E4-59F1

Limits computationally determined from genome sequence between P{PZ}Dcp-1⁰²¹³² and P{EP}apt^EP2339&P{PZ}apt⁰⁶³⁶⁹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

59F-59F

(Linassier, 1996.8.8)

59F-59F

(determined by in situ hybridisation)

(MacDougall and Linassier, 1995.10.6, MacDougall et al., 1995)

Experimentally Determined Recombination Data

Location

2-103

(FlyBase, 2016)

2-108.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (17)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

18214

w¹¹¹⁸; PBac{RB}Pi3K59F^e03961

64011

y¹ v¹; P{TRiP.HMJ30324}attP40

28473

y¹ w^*; P{EP}Pi3K59F^G6017

92484

y¹ v¹; P{U6.2-Pi3K59F.dgRNA}attP2

85758

y¹ sc^* v¹ sev²¹; P{TOE.GS01579}attP40

FlyORF

F001525

M{UAS-Pi3K59F.ORF}ZH-86Fb

F001151

M{UAS-Pi3K59F.ORF.3xHA}ZH-86Fb

NIG-Fly

HMJ30324

y¹ v¹; P{TRiP.HMJ30324}attP40/CyO

2RG-0147

y² VhaAC39-1^cho-1 v¹; P{U6-Pi3K59F.NIG.sgRNA}attP40/CyO

VDRC

v330123

P{VSH330123}attP40

v100296

P{KK107602}VIE-260B

v341139

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00160}attP40/CyO-GFP

More stocks available...

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (84)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0015277

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0015277

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Molecular cloning and biochemical characterisation of Pi3K59F.

(Linassier et al., 1997)

Identified by PCR using degenerate primers based on regions of strong amino acid homology found within the putative lipid kinase domain of mammalian p110α and yeast Vps34p.

(MacDougall et al., 1995)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Pi3K59F CG5373

(Zhang, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (26)

Reported As

Symbol Synonym

CG5373

(National Institute of Genetics Fly Stocks, 2022-, Trivedi et al., 2020, Meadows, 2017.1.11, Balakrishnan et al., 2015, Schertel et al., 2013, Érdi et al., 2012, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Abe et al., 2009, Keleman et al., 2009.8.5, Perkins et al., 2009, Qin et al., 2008, Saleh et al., 2006, Morrison et al., 2000)

DmVps34

(Melani et al., 2017, Miller et al., 2010, Leevers et al., 1995)

PI(3)K

(Fuss et al., 2007, Edgar, 1999, Lehner, 1999)

PI3K

(Wang et al., 2022, Wenner and Pekala, 2022, Gautam et al., 2021, Kannangara et al., 2021, Lam Wong and Verheyen, 2021, Morin-Poulard et al., 2021, Oliveira et al., 2021, Sciambra and Chtarbanova, 2021, Ding et al., 2020, Jasper, 2020, Lee et al., 2020, Mariano et al., 2020, Pan et al., 2020, Pastor-Pareja, 2020, Texada et al., 2020, Gupta and Ray, 2017, Denton et al., 2015, Nirala et al., 2013, Delanoue et al., 2010, Ahrens et al., 2007, Hoshizaki and Gibbs, 2007, Howlett et al., 2007, Lasko and Sonenberg, 2007, Lavery and Stern, 2007, Caldwell et al., 2006, Edgar, 2006, Liu and Lehmann, 2006, Luo et al., 2006, Martin-Pena et al., 2006, Shiojima and Walsh, 2006, McNeill and Bateman, 2005, Scott et al., 2005, Grewal and Saucedo, 2004, Leopold, 2004, Dickson, 2003, Gumienny and Padgett, 2003, Li et al., 2003, Longo and Finch, 2003, Luque et al., 2003, Neufeld, 2003, Nijhout, 2003, Saucedo et al., 2003, Saucedo and Edgar, 2002, Gingras et al., 2001, Jünger et al., 2001, Tapon et al., 2001, Leevers, 1999, Stambolic et al., 1999)

PI3K-59F

(MacDougall et al., 1998, Linassier, 1996.8.8, MacDougall and Linassier, 1995.10.6)

PI3K59F

(Ghosh et al., 2023, Gerenu et al., 2021, Sun and Stathopoulos, 2018, Abe et al., 2009)

PI3K_59F

(Linassier et al., 1997, Vanhaesebroeck et al., 1997, Leevers et al., 1996, MacDougall et al., 1995)

PI₃K

(Hardie, 2003)

PIK359F

(Jouette et al., 2017)

Pi3K

(Cui et al., 2020, Malita and Rewitz, 2020)

Pi3K59F

(Yu et al., 2024, Demir and Kacew, 2023, Gui et al., 2023, Liu et al., 2023, Huang et al., 2022, Lu et al., 2022, Kim and O'Connor, 2021, Lebo et al., 2021, Denton et al., 2020, Ghosh et al., 2020, Gohel et al., 2020, Qin et al., 2020, Hughes and Simmonds, 2019, Meltzer et al., 2019, Billes et al., 2018, Billmann et al., 2018, Melvin et al., 2018, O'Keefe and Denton, 2018, Galluzzi et al., 2017, Transgenic RNAi Project members, 2017-, Kuleesha et al., 2016, M'Angale and Staveley, 2016, Wan et al., 2015, Xu et al., 2015, Zirin et al., 2015, Schertel et al., 2013, Yin et al., 2013, Érdi et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Qin et al., 2011, Shulman et al., 2011, Wasbrough et al., 2010, Zirin and Perrimon, 2010, Qin et al., 2008, Spresser et al., 2008, Saleh et al., 2006)

Pi3K59F (Vps34)

(van de Leemput et al., 2022)

Pi3K59F/Vps34

(Denton et al., 2020)

Pi3K_59F

(Juhász et al., 2008)

Pi3k59F

(Dobson et al., 2016)

VPS34

(Cox et al., 2021, Denton et al., 2015, Abe et al., 2006)

Vps34

(Gui et al., 2023, Koehler and Huber, 2023, Szabó et al., 2023, Wang et al., 2023, Wang et al., 2022, Xu et al., 2022, Nagai et al., 2021, Allen et al., 2020, Csizmadia and Juhász, 2020, Gohel et al., 2020, Murakawa et al., 2020, Sênos Demarco et al., 2020, Xu et al., 2020, Bhattacharjee et al., 2019, Khezri and Rusten, 2019, Billes et al., 2018, Csizmadia et al., 2018, Hauswirth et al., 2018, O'Keefe and Denton, 2018, Pandey et al., 2018, Kim et al., 2017, Manent et al., 2017, Melani et al., 2017, O'Farrell et al., 2017, Wen et al., 2017, Hemalatha et al., 2016, Li et al., 2016, Pueyo et al., 2016, Schmid et al., 2016, Xu et al., 2015, Zhang and Baehrecke, 2015, Banreti et al., 2014, Mulakkal et al., 2014, Nagy et al., 2014, Bechtel et al., 2013, Chang et al., 2013, Lőw et al., 2013, Moy and Cherry, 2013, Nagy et al., 2013, Shravage et al., 2013, Yousefian et al., 2013, Zhang et al., 2013, Barth et al., 2012, Érdi et al., 2012, Pircs et al., 2012, Ribeiro et al., 2011, Chang and Neufeld, 2010, Miller et al., 2010, Velichkova et al., 2010, Juhász et al., 2008, Naps and Neufeld, 2008)

dPI3K

(Pospisilik et al., 2010, Oldham et al., 2005, Jorgensen and Tyers, 2004, Kim et al., 2004, Jacinto and Hall, 2003)

dVps34

(Bourouis et al., 2019, Abe et al., 2009)

pi3k59f

(Valko et al., 2022)

vps34

(Hu et al., 2022, Valko et al., 2022, Jouette et al., 2017, O'Farrell et al., 2017, Balakrishnan et al., 2015, Issman-Zecharya and Schuldiner, 2014, Nezis et al., 2010, Sagona et al., 2010)

Name Synonyms

PI 3-kinase

(Hardie, 2003, Day and Lawrence, 2000)

PI3-kinase

(Wilson and Leptin, 2000)

Phosphatidylinositol 3-kinase 59F

Phosphotidylinositol 3 kinase 59F

(Harbison et al., 2005, Leevers et al., 1995)

dPI 3-kinase

(Marygold and Leevers, 2002)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 69 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37733

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9W1M7

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9W1M7

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015277

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37733/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015277

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015277

FlyMine - An integrated database for Drosophila genomics

FBgn0015277

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG5373

MARRVEL_MODEL - MARRVEL (model organism gene)

37733

PDB - An information portal to biological macromolecular structures

Linkouts

BioGRID - A database of protein and genetic interactions.

63333

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0015277

DroID - A comprehensive database of gene and protein interactions.

FBgn0015277

DRSC - Results frm RNAi screens

FBgn0015277

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pi3K59F_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015277

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0015277

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

3-phosphoinositide biosynthesis

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5373

Flygut - An atlas of the Drosophila adult midgut

FBgn0015277

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0015277

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC000620

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

37733

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P91635

References (218)

The following external sites may use different assemblies or annotations than FlyBase.

-1.31

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